Detailed information NTG0302013

Bioinformatically predicted

Overview

• Name: comE/blpR
• Type: Regulator
• Locus tag: ETT60_RS02195
• Genome accession: NZ_CP035439
• Coordinates: 403324..404073 (+)
• Length: 249 a.a.
• NCBI ID: WP_030126108.1
• Uniprot ID: A0A223M029
• Organism: Streptococcus pyogenes strain emm77
• Function: activate transcription of early competence genes; regulation of comX expression (predicted from homology) Competence regulation

Genomic Context

Location: 398324..409073

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ETT60_RS09420 (ETT60_02160)	-	398667..398978 (+)	312	WP_227868558.1	CPBP family intramembrane glutamic endopeptidase	-
ETT60_RS09370	-	399063..399191 (+)	129	WP_002994583.1	hypothetical protein	-
ETT60_RS02165 (ETT60_02165)	-	399589..399816 (+)	228	WP_028797129.1	Blp family class II bacteriocin	-
ETT60_RS02170 (ETT60_02170)	-	399865..400182 (+)	318	WP_002994587.1	hypothetical protein	-
ETT60_RS09280	-	400365..401392 (+)	1028	Protein_367	IS3 family transposase	-
ETT60_RS02190 (ETT60_02190)	-	401601..403076 (+)	1476	Protein_368	IS1182 family transposase	-
ETT60_RS02195 (ETT60_02195)	comE/blpR	403324..404073 (+)	750	WP_030126108.1	response regulator transcription factor	Regulator
ETT60_RS02200 (ETT60_02200)	-	404074..405408 (+)	1335	WP_063631787.1	sensor histidine kinase	-
ETT60_RS02205 (ETT60_02205)	-	405556..405681 (-)	126	WP_011017462.1	ComC/BlpC family leader-containing pheromone/bacteriocin	-
ETT60_RS02210 (ETT60_02210)	-	405758..407122 (-)	1365	WP_129284364.1	bacteriocin secretion accessory protein	-

Sequence

Protein

• Length: 249 a.a.
• Molecular weight: 29141.44 Da
• Isoelectric Point: 5.8302

>NTDB_id=302013 ETT60_RS02195 WP_030126108.1 403324..404073(+) (comE/blpR) [Streptococcus pyogenes strain emm77]
MNIFVLEDDFLHQTRIEKIIYKILTDNKLEVNHLEVYGKPNQLLEDISERGRHQLFFLDIDIKGEDKKGMEIAVEIRNKD
PHAVIVFVTTHSEFMPVSFQYQVSALDFIDKELPEELFSHRIEKAITYVQDNQGKTLAEDSFVFTNVKSQIQVSFSDLLY
IETSLIPHKLILYSTKQRVEFYGQLSEIVEQDDRLFQCHRSFVVNPYNISSIDRSERLVYLKGGLSCIVSRLKIRSLIKV
VEELHTKEK

Nucleotide

• Length: 750 bp

>NTDB_id=302013 ETT60_RS02195 WP_030126108.1 403324..404073(+) (comE/blpR) [Streptococcus pyogenes strain emm77]
ATGAATATTTTTGTCTTAGAGGACGATTTTTTACATCAAACCAGAATTGAAAAAATTATTTATAAGATTTTGACTGATAA
TAAGTTGGAGGTTAACCACTTGGAAGTTTATGGTAAACCCAATCAGCTGCTTGAAGATATATCAGAGAGAGGAAGACATC
AGCTTTTTTTTCTTGACATTGACATTAAGGGAGAAGATAAAAAAGGAATGGAAATTGCTGTGGAGATTAGAAATAAAGAT
CCGCACGCAGTGATAGTTTTTGTGACCACACATTCAGAATTTATGCCGGTATCTTTTCAATACCAGGTTTCAGCTTTAGA
CTTTATTGACAAAGAGTTGCCAGAGGAGTTATTTAGTCATCGCATTGAAAAAGCTATAACTTATGTACAAGATAACCAAG
GAAAAACGTTAGCGGAGGATTCATTTGTTTTTACTAATGTAAAGTCCCAGATACAAGTCTCCTTTTCAGATTTGCTGTAT
ATTGAAACCTCACTAATTCCCCATAAACTCATTCTCTATTCTACTAAACAAAGAGTAGAATTTTATGGCCAATTATCAGA
AATTGTTGAGCAAGATGATCGATTATTTCAATGTCATCGTTCCTTTGTTGTCAATCCCTATAATATATCATCAATAGATA
GATCCGAACGGTTAGTTTATTTAAAGGGGGGATTATCTTGTATTGTTTCGAGATTGAAAATACGATCATTAATAAAAGTA
GTAGAAGAGTTGCACACTAAGGAGAAATAG

Domains

Predicted by InterProScan.

• LytTr DNA-binding domain (LytTR) (150-239)

• Response regulator receiver domain (Response_reg) (3-119)

3D structure

• AlphaFold DB: A0A223M029

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comE/blpR	Streptococcus mutans UA159	54.098	97.992	0.53
comE/comE2	Streptococcus gordonii strain NCTC7865	40.476	100	0.41
comE/comE1	Streptococcus gordonii str. Challis substr. CH1	40.476	100	0.41
comE/comE1	Streptococcus equinus JB1	42.437	95.582	0.406
comE/comE2	Streptococcus equinus JB1	40.574	97.992	0.398
comE	Streptococcus mitis NCTC 12261	38.4	100	0.386
comE	Streptococcus mitis SK321	38.4	100	0.386
comE	Streptococcus pneumoniae TIGR4	38	100	0.382
comE	Streptococcus pneumoniae Rx1	38	100	0.382
comE	Streptococcus pneumoniae D39	38	100	0.382
comE	Streptococcus pneumoniae R6	38	100	0.382
comE	Streptococcus infantis strain Atu-4	37.903	99.598	0.378