Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   DMR38_RS20905 Genome accession   NZ_CP029758
Coordinates   4447751..4448158 (-) Length   135 a.a.
NCBI ID   WP_127723583.1    Uniprot ID   -
Organism   Clostridium sp. AWRP     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 4415494..4456274 4447751..4448158 within 0


Gene organization within MGE regions


Location: 4415494..4456274
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DMR38_RS20710 (DMR38_20740) - 4415494..4415814 (+) 321 WP_063557014.1 hypothetical protein -
  DMR38_RS20715 (DMR38_20745) - 4416002..4416298 (-) 297 WP_243124382.1 CPBP family intramembrane glutamic endopeptidase -
  DMR38_RS20720 (DMR38_20750) - 4416295..4416636 (-) 342 WP_127723519.1 hypothetical protein -
  DMR38_RS22040 - 4416680..4416889 (+) 210 WP_127723521.1 hypothetical protein -
  DMR38_RS22465 - 4417105..4417473 (+) 369 WP_243124383.1 transposase -
  DMR38_RS20740 (DMR38_20770) - 4418187..4418837 (-) 651 WP_243124385.1 DUF4352 domain-containing protein -
  DMR38_RS20745 (DMR38_20775) - 4418952..4419164 (-) 213 WP_127723523.1 DNA-binding protein -
  DMR38_RS22045 - 4419359..4419496 (-) 138 WP_175413080.1 hypothetical protein -
  DMR38_RS20750 (DMR38_20780) - 4419624..4420175 (-) 552 WP_127723525.1 hypothetical protein -
  DMR38_RS20755 (DMR38_20785) - 4420178..4420993 (-) 816 WP_127723527.1 GH25 family lysozyme -
  DMR38_RS20760 (DMR38_20790) - 4421006..4421269 (-) 264 WP_127723529.1 hemolysin XhlA family protein -
  DMR38_RS22050 - 4421312..4422238 (-) 927 WP_175413081.1 hypothetical protein -
  DMR38_RS20770 (DMR38_20800) - 4422250..4422957 (-) 708 WP_127723531.1 hypothetical protein -
  DMR38_RS22165 (DMR38_20805) - 4422970..4423917 (-) 948 WP_127723533.1 hypothetical protein -
  DMR38_RS20780 (DMR38_20810) - 4423940..4424482 (-) 543 WP_127723535.1 hypothetical protein -
  DMR38_RS20785 (DMR38_20815) - 4424472..4426148 (-) 1677 WP_127723537.1 phage tail spike protein -
  DMR38_RS20790 (DMR38_20820) - 4426148..4426870 (-) 723 WP_127723539.1 distal tail protein Dit -
  DMR38_RS20795 (DMR38_20825) - 4426872..4431359 (-) 4488 WP_127723541.1 phage tail tape measure protein -
  DMR38_RS20800 (DMR38_20830) - 4431359..4431553 (-) 195 WP_127723543.1 hypothetical protein -
  DMR38_RS20805 (DMR38_20835) - 4431670..4432038 (-) 369 WP_127723545.1 hypothetical protein -
  DMR38_RS20810 (DMR38_20840) - 4432087..4432980 (-) 894 WP_127723547.1 SwmB domain-containing protein -
  DMR38_RS20815 (DMR38_20845) - 4432987..4433268 (-) 282 WP_243124386.1 hypothetical protein -
  DMR38_RS20820 (DMR38_20850) - 4433367..4433747 (-) 381 WP_127723549.1 HK97-gp10 family putative phage morphogenesis protein -
  DMR38_RS20825 (DMR38_20855) - 4433737..4434057 (-) 321 WP_127723551.1 hypothetical protein -
  DMR38_RS20830 (DMR38_20860) - 4434057..4434371 (-) 315 WP_127723553.1 phage head-tail connector protein -
  DMR38_RS20835 (DMR38_20865) - 4434402..4434635 (-) 234 WP_127723555.1 Rho termination factor N-terminal domain-containing protein -
  DMR38_RS20840 (DMR38_20870) - 4434635..4435558 (-) 924 WP_127723557.1 hypothetical protein -
  DMR38_RS20845 (DMR38_20875) - 4435574..4436170 (-) 597 WP_127723559.1 DUF4355 domain-containing protein -
  DMR38_RS20850 (DMR38_20880) - 4436310..4436546 (-) 237 WP_127723561.1 hypothetical protein -
  DMR38_RS20855 (DMR38_20885) - 4436548..4437807 (-) 1260 WP_175413082.1 minor capsid protein -
  DMR38_RS20860 (DMR38_20890) - 4437785..4439143 (-) 1359 WP_127723566.1 phage portal protein -
  DMR38_RS20865 (DMR38_20895) - 4439156..4440877 (-) 1722 WP_127723568.1 hypothetical protein -
  DMR38_RS20870 (DMR38_20900) - 4440883..4441122 (-) 240 WP_175413083.1 hypothetical protein -
  DMR38_RS20875 (DMR38_20905) - 4441192..4441689 (-) 498 WP_127723572.1 hypothetical protein -
  DMR38_RS20880 (DMR38_20910) - 4441700..4442485 (-) 786 WP_127723574.1 endonuclease/exonuclease/phosphatase family protein -
  DMR38_RS20885 (DMR38_20915) - 4442749..4443153 (-) 405 WP_127723576.1 helix-turn-helix domain-containing protein -
  DMR38_RS20890 (DMR38_20920) - 4443298..4443720 (-) 423 WP_127723578.1 RNA polymerase subunit sigma -
  DMR38_RS22060 - 4443738..4443878 (-) 141 WP_175413084.1 hypothetical protein -
  DMR38_RS20895 (DMR38_20925) - 4444164..4446749 (-) 2586 WP_127723580.1 CHC2 zinc finger domain-containing protein -
  DMR38_RS22065 - 4446787..4446927 (-) 141 WP_175413085.1 hypothetical protein -
  DMR38_RS20900 (DMR38_20930) - 4446924..4447514 (-) 591 WP_175413151.1 ERCC4 domain-containing protein -
  DMR38_RS20905 (DMR38_20935) ssb 4447751..4448158 (-) 408 WP_127723583.1 single-stranded DNA-binding protein Machinery gene
  DMR38_RS20910 (DMR38_20940) - 4448171..4448791 (-) 621 WP_127723585.1 HD domain-containing protein -
  DMR38_RS20915 (DMR38_20945) bet 4448805..4449749 (-) 945 WP_127723587.1 phage recombination protein Bet -
  DMR38_RS20920 (DMR38_20950) - 4449749..4451428 (-) 1680 WP_127723589.1 DNA repair protein -
  DMR38_RS20925 (DMR38_20955) - 4451444..4451668 (-) 225 WP_127723591.1 hypothetical protein -
  DMR38_RS20930 (DMR38_20960) - 4451720..4452220 (-) 501 WP_127723593.1 helix-turn-helix transcriptional regulator -
  DMR38_RS22070 - 4452279..4452446 (-) 168 WP_175413086.1 hypothetical protein -
  DMR38_RS20935 (DMR38_20965) - 4452443..4452631 (-) 189 WP_127723595.1 hypothetical protein -
  DMR38_RS20940 (DMR38_20970) - 4452771..4453034 (-) 264 WP_127723597.1 hypothetical protein -
  DMR38_RS20945 (DMR38_20975) - 4453051..4453251 (-) 201 WP_243124387.1 hypothetical protein -
  DMR38_RS22080 - 4453254..4453406 (-) 153 WP_175413088.1 hypothetical protein -
  DMR38_RS20950 (DMR38_20980) - 4453393..4453581 (-) 189 WP_127723599.1 hypothetical protein -
  DMR38_RS22085 - 4453578..4453748 (-) 171 WP_175413089.1 hypothetical protein -
  DMR38_RS20955 (DMR38_20985) - 4453839..4454072 (-) 234 WP_127723601.1 helix-turn-helix domain-containing protein -
  DMR38_RS20960 (DMR38_20990) - 4454254..4454643 (+) 390 WP_127723603.1 helix-turn-helix transcriptional regulator -
  DMR38_RS20965 (DMR38_20995) - 4454669..4455112 (+) 444 WP_127723605.1 ImmA/IrrE family metallo-endopeptidase -
  DMR38_RS20970 (DMR38_21000) - 4455105..4456274 (+) 1170 WP_127723607.1 tyrosine-type recombinase/integrase -

Sequence


Protein


Download         Length: 135 a.a.        Molecular weight: 15154.88 Da        Isoelectric Point: 4.7719

>NTDB_id=295942 DMR38_RS20905 WP_127723583.1 4447751..4448158(-) (ssb) [Clostridium sp. AWRP]
MNRVILIGRLTKDPDLQFLPGNGTAVTKFTLAVDRRFKKEGQQEADFVPIVVWNKQAESAANYLTKGKLAGIAGRIQTRN
YEAKDGTKRYVTEVIAEEVQFLEWGDKVSNNSSQNNQQSNNADYVEVEDDGDIPF

Nucleotide


Download         Length: 408 bp        

>NTDB_id=295942 DMR38_RS20905 WP_127723583.1 4447751..4448158(-) (ssb) [Clostridium sp. AWRP]
TTGAATAGAGTTATTTTAATAGGAAGATTAACCAAAGATCCTGACTTGCAGTTTTTACCCGGCAATGGGACAGCAGTAAC
AAAGTTTACATTAGCAGTTGATAGAAGGTTTAAAAAAGAAGGACAGCAGGAAGCTGATTTTGTACCTATAGTGGTTTGGA
ATAAGCAAGCTGAGAGTGCTGCCAATTATTTAACTAAAGGTAAATTAGCAGGAATTGCAGGGAGAATTCAAACAAGAAAT
TATGAGGCAAAAGATGGTACTAAAAGATATGTTACTGAAGTTATTGCAGAAGAGGTTCAATTCTTAGAATGGGGAGATAA
AGTAAGTAATAATTCAAGTCAAAATAATCAACAAAGTAATAATGCTGATTATGTAGAAGTTGAGGACGACGGGGATATAC
CATTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

39.766

100

0.504

  ssbB Streptococcus sobrinus strain NIDR 6715-7

45.926

100

0.459

  ssbA Bacillus subtilis subsp. subtilis str. 168

59.615

77.037

0.459

  ssbB/cilA Streptococcus mitis NCTC 12261

42.963

100

0.43

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.222

100

0.422

  ssbA Streptococcus mutans UA159

42.222

100

0.422

  ssbB/cilA Streptococcus pneumoniae Rx1

41.481

100

0.415

  ssbB/cilA Streptococcus mitis SK321

41.481

100

0.415

  ssbB/cilA Streptococcus pneumoniae D39

41.481

100

0.415

  ssbB/cilA Streptococcus pneumoniae R6

41.481

100

0.415

  ssbB Bacillus subtilis subsp. subtilis str. 168

51.923

77.037

0.4


Multiple sequence alignment