Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   CSB84_RS04750 Genome accession   NZ_CP029663
Coordinates   889558..889986 (+) Length   142 a.a.
NCBI ID   WP_000934385.1    Uniprot ID   A0A2K0CLZ8
Organism   Staphylococcus aureus strain AR_0228     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 880197..926862 889558..889986 within 0


Gene organization within MGE regions


Location: 880197..926862
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSB84_RS04665 (CSB84_0824) sufB 880197..881594 (+) 1398 WP_001074405.1 Fe-S cluster assembly protein SufB -
  CSB84_RS04670 (CSB84_0825) - 881662..882711 (-) 1050 WP_001145726.1 tyrosine-type recombinase/integrase -
  CSB84_RS04675 (CSB84_0826) - 882824..883003 (+) 180 WP_000337826.1 hypothetical protein -
  CSB84_RS04680 (CSB84_0827) - 882983..883915 (-) 933 WP_000392186.1 hypothetical protein -
  CSB84_RS04685 (CSB84_0828) - 883947..884672 (-) 726 WP_000661437.1 PH domain-containing protein -
  CSB84_RS04690 (CSB84_0829) - 884700..885374 (-) 675 WP_000775188.1 ImmA/IrrE family metallo-endopeptidase -
  CSB84_RS04695 (CSB84_0830) - 885391..885723 (-) 333 WP_001055143.1 helix-turn-helix transcriptional regulator -
  CSB84_RS04700 (CSB84_0831) - 885986..886180 (+) 195 WP_000108122.1 helix-turn-helix transcriptional regulator -
  CSB84_RS04705 (CSB84_0832) - 886180..886947 (+) 768 WP_001002757.1 phage antirepressor Ant -
  CSB84_RS04710 (CSB84_0833) - 886948..887172 (+) 225 WP_000187184.1 hypothetical protein -
  CSB84_RS04715 (CSB84_0834) - 887212..887661 (+) 450 WP_001094943.1 hypothetical protein -
  CSB84_RS04720 (CSB84_0835) - 887675..887896 (+) 222 WP_000977381.1 hypothetical protein -
  CSB84_RS04725 - 887889..888050 (+) 162 WP_000066011.1 DUF1270 domain-containing protein -
  CSB84_RS04730 (CSB84_0836) - 888144..888446 (+) 303 WP_000165363.1 DUF2482 family protein -
  CSB84_RS04735 (CSB84_0837) - 888451..888711 (+) 261 WP_000291090.1 DUF1108 family protein -
  CSB84_RS04740 (CSB84_0838) - 888721..888942 (+) 222 WP_000815401.1 DUF2483 family protein -
  CSB84_RS04745 (CSB84_0839) - 888935..889558 (+) 624 WP_000139720.1 DUF1071 domain-containing protein -
  CSB84_RS04750 (CSB84_0840) ssbA 889558..889986 (+) 429 WP_000934385.1 single-stranded DNA-binding protein Machinery gene
  CSB84_RS04755 (CSB84_0841) - 890000..890674 (+) 675 WP_001124438.1 putative HNHc nuclease -
  CSB84_RS15390 - 890671..890820 (+) 150 WP_001081076.1 hypothetical protein -
  CSB84_RS04760 (CSB84_0842) - 890813..891094 (-) 282 WP_000414755.1 hypothetical protein -
  CSB84_RS04765 (CSB84_0843) - 891160..891930 (+) 771 WP_000190254.1 conserved phage C-terminal domain-containing protein -
  CSB84_RS04770 (CSB84_0844) - 891940..892719 (+) 780 WP_000803062.1 ATP-binding protein -
  CSB84_RS04775 (CSB84_0845) - 892713..892871 (+) 159 WP_000256589.1 hypothetical protein -
  CSB84_RS04780 (CSB84_0846) - 892884..893105 (+) 222 WP_001123695.1 DUF3269 family protein -
  CSB84_RS04785 (CSB84_0847) - 893115..893519 (+) 405 WP_000049794.1 DUF1064 domain-containing protein -
  CSB84_RS04790 (CSB84_0848) - 893524..893709 (+) 186 WP_001187243.1 DUF3113 family protein -
  CSB84_RS04795 (CSB84_0849) - 893710..894015 (+) 306 WP_000101252.1 hypothetical protein -
  CSB84_RS04800 (CSB84_0850) - 894143..894499 (+) 357 WP_000029376.1 SA1788 family PVL leukocidin-associated protein -
  CSB84_RS04805 (CSB84_0851) - 894503..894745 (+) 243 WP_000131376.1 SAV1978 family virulence-associated passenger protein -
  CSB84_RS04810 (CSB84_0852) - 894754..895125 (+) 372 WP_001557190.1 hypothetical protein -
  CSB84_RS04815 (CSB84_0853) - 895118..895372 (+) 255 WP_001065060.1 DUF1024 family protein -
  CSB84_RS04820 (CSB84_0854) - 895359..895529 (+) 171 WP_000714412.1 hypothetical protein -
  CSB84_RS04825 (CSB84_0855) - 895522..896055 (+) 534 WP_000185647.1 dUTP pyrophosphatase -
  CSB84_RS04830 (CSB84_0856) - 896092..896298 (+) 207 WP_000617154.1 DUF1381 domain-containing protein -
  CSB84_RS04835 (CSB84_0857) - 896295..896489 (+) 195 WP_000132921.1 hypothetical protein -
  CSB84_RS04840 (CSB84_0858) - 896486..896689 (+) 204 WP_001072795.1 hypothetical protein -
  CSB84_RS04845 (CSB84_0859) - 896682..896918 (+) 237 WP_000608278.1 hypothetical protein -
  CSB84_RS04850 (CSB84_0860) - 896911..897084 (+) 174 WP_000595257.1 transcriptional activator RinB -
  CSB84_RS04855 - 897085..897186 (+) 102 Protein_852 hypothetical protein -
  CSB84_RS04860 (CSB84_0861) - 897255..897677 (+) 423 WP_000162701.1 RinA family phage transcriptional activator -
  CSB84_RS04865 (CSB84_0862) - 897865..898359 (+) 495 WP_001038244.1 terminase small subunit -
  CSB84_RS04870 (CSB84_0863) - 898362..899657 (+) 1296 WP_000273011.1 PBSX family phage terminase large subunit -
  CSB84_RS04875 (CSB84_0864) - 899668..901198 (+) 1531 Protein_856 phage portal protein -
  CSB84_RS04880 - 901260..902203 (+) 944 Protein_857 minor capsid protein -
  CSB84_RS04885 (CSB84_0867) - 902276..902446 (+) 171 WP_000072205.1 hypothetical protein -
  CSB84_RS15555 - 902474..902567 (+) 94 Protein_859 hypothetical protein -
  CSB84_RS04890 - 902555..903171 (+) 617 Protein_860 DUF4355 domain-containing protein -
  CSB84_RS04895 - 903185..904155 (+) 971 Protein_861 phage major capsid protein -
  CSB84_RS04900 - 904177..904461 (+) 285 Protein_862 hypothetical protein -
  CSB84_RS04905 - 904470..904801 (+) 332 Protein_863 phage head-tail connector protein -
  CSB84_RS04910 - 904798..905096 (+) 299 Protein_864 hypothetical protein -
  CSB84_RS04915 - 905096..905441 (+) 346 Protein_865 HK97-gp10 family putative phage morphogenesis protein -
  CSB84_RS04920 (CSB84_0878) - 905453..905835 (+) 383 Protein_866 hypothetical protein -
  CSB84_RS04925 (CSB84_0879) - 905915..906431 (+) 517 Protein_867 phage major tail protein, TP901-1 family -
  CSB84_RS04930 - 906493..906857 (+) 365 Protein_868 tail assembly chaperone -
  CSB84_RS04935 - 906887..907230 (+) 344 Protein_869 hypothetical protein -
  CSB84_RS15560 - 907261..910700 (+) 3440 Protein_870 hypothetical protein -
  CSB84_RS04965 - 910713..911679 (+) 967 Protein_871 phage tail family protein -
  CSB84_RS04970 - 911663..913559 (+) 1897 Protein_872 SGNH/GDSL hydrolase family protein -
  CSB84_RS15405 - 913574..915481 (+) 1908 Protein_873 hypothetical protein -
  CSB84_RS04985 (CSB84_0894) - 915481..917304 (+) 1824 WP_000259619.1 BppU family phage baseplate upper protein -
  CSB84_RS04990 (CSB84_0895) - 917304..917681 (+) 378 WP_000705896.1 DUF2977 domain-containing protein -
  CSB84_RS04995 (CSB84_0896) - 917685..917858 (+) 174 WP_000782200.1 XkdX family protein -
  CSB84_RS05000 (CSB84_0897) - 917898..918197 (+) 300 WP_000466778.1 DUF2951 domain-containing protein -
  CSB84_RS05005 (CSB84_0898) - 918334..920232 (+) 1899 WP_000524019.1 glucosaminidase domain-containing protein -
  CSB84_RS05010 (CSB84_0899) - 920245..921483 (+) 1239 WP_000276662.1 BppU family phage baseplate upper protein -
  CSB84_RS05015 (CSB84_0900) - 921489..921884 (+) 396 WP_000398868.1 hypothetical protein -
  CSB84_RS05020 (CSB84_0901) - 921940..922377 (+) 438 WP_000354132.1 phage holin -
  CSB84_RS05025 (CSB84_0902) - 922358..923803 (+) 1446 WP_001148130.1 SH3 domain-containing protein -
  CSB84_RS05030 (CSB84_0903) - 924209..924871 (+) 663 WP_000385047.1 hypothetical protein -
  CSB84_RS05035 (CSB84_0904) - 924886..925527 (+) 642 WP_001190816.1 hypothetical protein -
  CSB84_RS05040 - 925628..925708 (-) 81 WP_100250272.1 hypothetical protein -
  CSB84_RS05045 (CSB84_0905) - 926055..926216 (+) 162 WP_001005407.1 SE1561 family protein -
  CSB84_RS05050 (CSB84_0906) - 926347..926862 (+) 516 WP_000163283.1 type 1 glutamine amidotransferase domain-containing protein -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 15872.58 Da        Isoelectric Point: 8.4825

>NTDB_id=294788 CSB84_RS04750 WP_000934385.1 889558..889986(+) (ssbA) [Staphylococcus aureus strain AR_0228]
MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGEREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTR
SYENKVGQRVFVTEVVADSVQFLEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF

Nucleotide


Download         Length: 429 bp        

>NTDB_id=294788 CSB84_RS04750 WP_000934385.1 889558..889986(+) (ssbA) [Staphylococcus aureus strain AR_0228]
ATGTTAAACAGAGCAGTATTAGTAGGACGCTTAACAAAAGACCCAGAATTAAGAAGCGCGCCAAATGGCGTAAATGTAGG
TACATTCACATTGGCAGTAAACAGAACATTCACGAATGCTCAAGGCGAGCGTGAAGCAGATTTTATAAACGTAGTAGTGT
TCAAGAAACAAGCTGAAAATGTTAAAAACTACCTTTCTAAAGGGTCGCTGGCAGGTGTAGACGGGCGACTACAAACACGT
AGCTACGAAAATAAAGTCGGGCAACGTGTATTTGTGACAGAAGTAGTAGCGGACAGTGTTCAATTCTTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAAGACAAACTCAAACTGGTAATAATCCTTTTGATAATACCACTG
CGATTACTGATGATGACTTACCGTTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2K0CLZ8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

58.14

100

0.704

  ssb Latilactobacillus sakei subsp. sakei 23K

50.588

100

0.606

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

74.648

0.437

  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.958

100

0.423

  ssbA Streptococcus mutans UA159

40.845

100

0.408

  ssbB/cilA Streptococcus mitis NCTC 12261

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae Rx1

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae D39

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae R6

39.437

100

0.394

  ssbB/cilA Streptococcus mitis SK321

39.437

100

0.394


Multiple sequence alignment