Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   DKG76_RS13420 Genome accession   NZ_CP029465
Coordinates   2720418..2721035 (-) Length   205 a.a.
NCBI ID   WP_003237107.1    Uniprot ID   A0A9W5PDV8
Organism   Bacillus inaquosorum strain KCTC 13429     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2715418..2726035
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DKG76_RS13395 (DKG76_13395) rpsT 2715720..2715986 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  DKG76_RS13400 (DKG76_13400) holA 2716001..2717044 (-) 1044 WP_003237104.1 DNA polymerase III subunit delta -
  DKG76_RS21955 - 2717084..2717233 (-) 150 WP_003229985.1 hypothetical protein -
  DKG76_RS13405 (DKG76_13405) - 2717274..2717408 (+) 135 WP_003229983.1 YqzM family protein -
  DKG76_RS13410 (DKG76_13410) comEC 2717448..2719778 (-) 2331 WP_032731956.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  DKG76_RS13415 (DKG76_13415) - 2719782..2720351 (-) 570 WP_003237106.1 ComE operon protein 2 -
  DKG76_RS13420 (DKG76_13420) comEA 2720418..2721035 (-) 618 WP_003237107.1 helix-hairpin-helix domain-containing protein Machinery gene
  DKG76_RS13425 (DKG76_13425) comER 2721119..2721940 (+) 822 WP_003237109.1 late competence protein ComER -
  DKG76_RS13430 (DKG76_13430) - 2722006..2722749 (-) 744 WP_003237111.1 class I SAM-dependent methyltransferase -
  DKG76_RS13435 (DKG76_13435) rsfS 2722746..2723102 (-) 357 WP_003237112.1 ribosome silencing factor -
  DKG76_RS13440 (DKG76_13440) yqeK 2723120..2723680 (-) 561 WP_003237115.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  DKG76_RS13445 (DKG76_13445) - 2723670..2724239 (-) 570 WP_003237117.1 nicotinate-nucleotide adenylyltransferase -
  DKG76_RS13450 (DKG76_13450) yhbY 2724251..2724541 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  DKG76_RS13455 (DKG76_13455) aroE 2724535..2725377 (-) 843 WP_003237119.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21808.58 Da        Isoelectric Point: 4.8354

>NTDB_id=292747 DKG76_RS13420 WP_003237107.1 2720418..2721035(-) (comEA) [Bacillus inaquosorum strain KCTC 13429]
MNWLNQHKKTIILAASAAVFIALFFIFSSGKNKEPVKQAVSTETENAGVKQEAVKDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGASEQADEMQVNLAELLQDGTVVYIPKKGEETAVQQGAGGAVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=292747 DKG76_RS13420 WP_003237107.1 2720418..2721035(-) (comEA) [Bacillus inaquosorum strain KCTC 13429]
ATGAATTGGTTGAATCAGCATAAAAAAACAATTATTTTAGCGGCTTCTGCGGCTGTTTTCATAGCGTTATTTTTCATCTT
TTCCAGCGGAAAAAACAAAGAGCCGGTGAAGCAGGCTGTATCAACTGAGACAGAAAATGCGGGGGTAAAGCAGGAAGCAG
TAAAAGACGAGTCAAACGAAACAATTGTGATTGACATTAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGAGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGGCCAGTGAACAAGCAGACGAAATGCAAGTGAACTTGGCGGA
GCTGCTTCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAGACGGCGGTGCAGCAAGGAGCCGGAGGCGCTG
TTCAAAGCGATGGAGGGAAGGGAGCGCTCGTAAATATCAATACAGCCACCTTAGAGGAATTACAAGGCATCTCAGGGGTG
GGACCGTCAAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGGCGTTTCCAGACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

90.732

100

0.907
  comEA/celA/cilE Streptococcus mitis SK321

38.835

100

0.39
  comEA/celA/cilE Streptococcus pneumoniae Rx1

38.164

100

0.385
  comEA/celA/cilE Streptococcus pneumoniae D39

38.164

100

0.385
  comEA/celA/cilE Streptococcus mitis NCTC 12261

38.164

100

0.385
  comEA/celA/cilE Streptococcus pneumoniae R6

38.164

100

0.385
  comEA Staphylococcus aureus MW2

36.967

100

0.38
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

37.681

100

0.38
  comEA Staphylococcus aureus N315

35.545

100

0.366
  comEA Lactococcus lactis subsp. cremoris KW2

33.945

100

0.361

Multiple sequence alignment