Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HMF8227_RS12205 Genome accession   NZ_CP029347
Coordinates   2603781..2604998 (+) Length   405 a.a.
NCBI ID   WP_109340438.1    Uniprot ID   A0A2S2E5G9
Organism   Saliniradius amylolyticus strain HMF8227     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2598781..2609998
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HMF8227_RS12180 (HMF8227_02451) pdhR 2599793..2600548 (-) 756 WP_109340435.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  HMF8227_RS12185 (HMF8227_02452) ampE 2600730..2601554 (-) 825 WP_162558599.1 beta-lactamase regulator AmpE -
  HMF8227_RS12190 (HMF8227_02453) - 2601758..2602231 (+) 474 WP_109341105.1 retropepsin-like aspartic protease family protein -
  HMF8227_RS12195 (HMF8227_02454) nadC 2602235..2603083 (+) 849 WP_109340437.1 carboxylating nicotinate-nucleotide diphosphorylase -
  HMF8227_RS12200 (HMF8227_02455) - 2603278..2603709 (+) 432 WP_162558600.1 pilin -
  HMF8227_RS12205 (HMF8227_02456) pilC 2603781..2604998 (+) 1218 WP_109340438.1 type II secretion system F family protein Machinery gene
  HMF8227_RS12210 (HMF8227_02457) pilD 2605051..2605932 (+) 882 WP_109340439.1 prepilin peptidase Machinery gene
  HMF8227_RS12215 (HMF8227_02458) coaE 2605929..2606528 (+) 600 WP_109340440.1 dephospho-CoA kinase -
  HMF8227_RS12220 (HMF8227_02459) zapD 2606631..2607383 (+) 753 WP_109340441.1 cell division protein ZapD -
  HMF8227_RS12225 (HMF8227_02460) yacG 2607388..2607615 (+) 228 WP_109340442.1 DNA gyrase inhibitor YacG -
  HMF8227_RS12230 (HMF8227_02461) mutT 2607605..2608003 (-) 399 WP_109340443.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43795.65 Da        Isoelectric Point: 9.7114

>NTDB_id=291843 HMF8227_RS12205 WP_109340438.1 2603781..2604998(+) (pilC) [Saliniradius amylolyticus strain HMF8227]
MAKQPALQIFTWQGKNRQGQNIDGELSAKNIMDARTQLRKKGVSVTKIKKYSKPLFGKGAAKIKAADICVVSRQIATMLS
AGVTLIQTIDMIADGANNAAMRKMLIEIGNDVKAGTPLSESLRKHPDHFDELYCDLVETGEQSGSLETIYDRIATYKEKA
EALKSKIKKAMFYPIAVIVVATIVTLILLIFVVPQFQEIFASFGAELPAFTLFVLGISQAIQNYGLYMAGALVAAAALFK
RAHKKSKKLRDKVDKQILKIPVIGEILKKASIARFTRTLSTTFAAGVPLIGALESAAGAAGNAVFRDAILTIRKEVSGGM
QMNTAMRDTGVFPDMVVQMVAIGEESGSVDDMLDKVATIYEREVDDLVDGLTALLEPMIMAVLGVVIGGLIIAMYLPIFQ
MGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=291843 HMF8227_RS12205 WP_109340438.1 2603781..2604998(+) (pilC) [Saliniradius amylolyticus strain HMF8227]
ATGGCCAAGCAACCAGCCTTACAAATTTTTACCTGGCAGGGTAAAAACCGTCAGGGCCAGAATATCGATGGCGAACTCTC
CGCCAAGAACATTATGGATGCCAGGACTCAGCTGCGTAAAAAAGGCGTTTCTGTCACCAAAATCAAAAAGTATTCCAAGC
CACTATTCGGCAAAGGCGCGGCCAAAATAAAGGCGGCGGATATCTGCGTTGTCTCGCGCCAGATCGCCACCATGCTAAGC
GCTGGCGTGACTCTGATCCAGACCATCGATATGATCGCCGACGGGGCTAACAATGCCGCTATGCGCAAGATGCTGATCGA
AATTGGTAATGATGTGAAGGCTGGCACCCCCTTATCCGAATCCTTGCGTAAACATCCCGATCATTTCGATGAACTTTATT
GCGACCTGGTAGAAACCGGTGAGCAATCAGGCTCACTGGAAACTATTTATGATCGTATTGCCACCTACAAAGAAAAGGCC
GAGGCTTTAAAGTCCAAGATTAAAAAAGCCATGTTCTATCCCATTGCGGTGATCGTTGTGGCGACCATTGTTACCTTGAT
TCTGCTTATTTTTGTCGTCCCTCAGTTTCAGGAAATCTTTGCCAGTTTCGGAGCCGAGTTGCCCGCATTCACTCTTTTTG
TTTTGGGTATCTCCCAGGCAATTCAGAATTATGGTTTGTATATGGCCGGTGCTCTGGTCGCTGCAGCGGCATTATTTAAA
CGGGCTCATAAAAAGTCTAAGAAGCTTAGGGACAAGGTGGATAAACAGATCCTGAAGATTCCTGTTATTGGTGAAATTCT
AAAAAAAGCCTCCATCGCACGCTTTACCCGCACCCTGTCCACGACCTTTGCCGCCGGTGTCCCCTTAATCGGCGCTCTGG
AATCGGCCGCTGGCGCAGCTGGTAACGCAGTGTTCCGGGATGCCATCTTAACCATTCGCAAGGAAGTCTCCGGTGGCATG
CAGATGAATACTGCCATGCGCGATACCGGCGTGTTTCCGGATATGGTAGTGCAAATGGTCGCTATCGGCGAAGAGTCCGG
ATCGGTGGACGATATGCTGGACAAGGTGGCCACCATCTACGAACGGGAAGTGGACGATCTCGTCGATGGCCTGACAGCCT
TGCTGGAGCCAATGATAATGGCCGTATTGGGTGTAGTGATCGGTGGTCTGATCATCGCTATGTACTTACCCATATTCCAG
ATGGGCAGTGTGGTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S2E5G9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

61.209

98.025

0.6
  pilC Acinetobacter baumannii D1279779

55.164

98.025

0.541
  pilC Acinetobacter baylyi ADP1

52.228

99.753

0.521
  pilC Legionella pneumophila strain ERS1305867

50.62

99.506

0.504
  pilC Vibrio cholerae strain A1552

45.614

98.519

0.449
  pilG Neisseria meningitidis 44/76-A

43.25

98.765

0.427
  pilG Neisseria gonorrhoeae MS11

43

98.765

0.425
  pilC Vibrio campbellii strain DS40M4

42.145

99.012

0.417
  pilC Thermus thermophilus HB27

36.725

99.506

0.365

Multiple sequence alignment