Detailed information NTG0291844

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HMF8227_RS12210
• Genome accession: NZ_CP029347
• Coordinates: 2605051..2605932 (+)
• Length: 293 a.a.
• NCBI ID: WP_109340439.1
• Uniprot ID: A0A2S2E5N4
• Organism: Saliniradius amylolyticus strain HMF8227
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2600051..2610932

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMF8227_RS12185 (HMF8227_02452)	ampE	2600730..2601554 (-)	825	WP_162558599.1	beta-lactamase regulator AmpE	-
HMF8227_RS12190 (HMF8227_02453)	-	2601758..2602231 (+)	474	WP_109341105.1	retropepsin-like aspartic protease family protein	-
HMF8227_RS12195 (HMF8227_02454)	nadC	2602235..2603083 (+)	849	WP_109340437.1	carboxylating nicotinate-nucleotide diphosphorylase	-
HMF8227_RS12200 (HMF8227_02455)	-	2603278..2603709 (+)	432	WP_162558600.1	pilin	-
HMF8227_RS12205 (HMF8227_02456)	pilC	2603781..2604998 (+)	1218	WP_109340438.1	type II secretion system F family protein	Machinery gene
HMF8227_RS12210 (HMF8227_02457)	pilD	2605051..2605932 (+)	882	WP_109340439.1	prepilin peptidase	Machinery gene
HMF8227_RS12215 (HMF8227_02458)	coaE	2605929..2606528 (+)	600	WP_109340440.1	dephospho-CoA kinase	-
HMF8227_RS12220 (HMF8227_02459)	zapD	2606631..2607383 (+)	753	WP_109340441.1	cell division protein ZapD	-
HMF8227_RS12225 (HMF8227_02460)	yacG	2607388..2607615 (+)	228	WP_109340442.1	DNA gyrase inhibitor YacG	-
HMF8227_RS12230 (HMF8227_02461)	mutT	2607605..2608003 (-)	399	WP_109340443.1	8-oxo-dGTP diphosphatase MutT	-
HMF8227_RS12235 (HMF8227_02462)	secA	2608003..2610696 (-)	2694	WP_109340444.1	preprotein translocase subunit SecA	-

Sequence

Protein

• Length: 293 a.a.
• Molecular weight: 32808.73 Da
• Isoelectric Point: 5.5310

>NTDB_id=291844 HMF8227_RS12210 WP_109340439.1 2605051..2605932(+) (pilD) [Saliniradius amylolyticus strain HMF8227]
MTEILTLLEVSPAFLLSLIAFISLMVGSFLNVVIYRLPVMMEREWQSEYQSYFHDQAAETDNSTKPFNLVKPDSTCPHCH
TPIKAIHNVPVLSWLFLKGRCAYCQTTIPARYPMVEALTTLLSVWVTWHLGYGPMLIPALMFTWLLIAMTFIDLDKMLLP
DQLTLPLLWLGLIGSLYWSPVSPEDAILGAAAGYLSLWSIYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQGLPVVILLS
SLVGAIVGLSQIAFKGQDHAKPIPFGPYLAAAGWLAFLYGDWLTEQYWQWVLA

Nucleotide

• Length: 882 bp

>NTDB_id=291844 HMF8227_RS12210 WP_109340439.1 2605051..2605932(+) (pilD) [Saliniradius amylolyticus strain HMF8227]
ATGACCGAGATTTTAACTCTACTGGAAGTTTCTCCGGCGTTTCTGCTTAGCCTCATCGCCTTTATCAGCTTAATGGTAGG
CAGTTTTCTCAATGTCGTCATCTACCGCTTGCCTGTGATGATGGAGCGAGAATGGCAAAGCGAATATCAAAGCTATTTCC
ATGACCAGGCAGCTGAAACTGACAACTCAACCAAACCCTTTAACCTGGTTAAACCGGACTCCACCTGTCCCCATTGTCAC
ACCCCCATTAAGGCAATTCACAATGTTCCCGTATTGAGCTGGCTCTTTCTGAAAGGCCGCTGCGCTTATTGCCAGACGAC
CATTCCTGCCCGCTACCCTATGGTGGAAGCTCTCACCACGCTGTTGTCGGTCTGGGTTACCTGGCATTTAGGCTATGGGC
CCATGCTCATTCCGGCATTGATGTTCACGTGGCTGCTCATCGCTATGACCTTTATCGATCTGGACAAAATGCTGCTACCG
GATCAACTCACCCTGCCCTTACTCTGGCTTGGGCTTATTGGCAGTCTGTACTGGTCTCCGGTTTCTCCTGAAGATGCCAT
CTTAGGAGCTGCTGCTGGCTATTTAAGCCTGTGGAGCATCTATTGGGGATTTAAGCTGCTGACTGGCAAAGAGGGTATGG
GCTATGGTGACTTTAAGCTGCTGGCTGCCTTAGGCGCCTGGCTAGGCTGGCAAGGCCTGCCAGTGGTGATTCTATTGTCG
TCCCTGGTTGGGGCCATCGTCGGTCTGTCGCAGATCGCCTTTAAGGGACAAGACCACGCCAAGCCTATTCCCTTCGGGCC
CTACTTAGCGGCTGCAGGCTGGCTGGCTTTCCTCTATGGTGACTGGCTGACAGAACAATACTGGCAGTGGGTTTTAGCAT
GA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (22-129)

• Type IV leader peptidase family (Peptidase_A24) (141-249)

3D structure

• AlphaFold DB: A0A2S2E5N4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	56.985	92.833	0.529
pilD	Vibrio campbellii strain DS40M4	51.724	98.976	0.512
pilD	Neisseria gonorrhoeae MS11	48.763	96.587	0.471
pilD	Acinetobacter nosocomialis M2	50.558	91.809	0.464
pilD	Acinetobacter baumannii D1279779	50.558	91.809	0.464