Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C3363_RS00935 Genome accession   NZ_CP029150
Coordinates   182002..183387 (+) Length   461 a.a.
NCBI ID   WP_010787006.1    Uniprot ID   -
Organism   Glaesserella parasuis strain GZ20170512     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 177002..188387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C3363_RS00910 (C3363_00930) gpt 177503..177982 (+) 480 WP_010787011.1 xanthine phosphoribosyltransferase -
  C3363_RS00915 (C3363_00935) gmk 178316..178942 (+) 627 WP_010787010.1 guanylate kinase -
  C3363_RS00920 (C3363_00940) radA 178995..180371 (-) 1377 WP_010787009.1 DNA repair protein RadA -
  C3363_RS00925 (C3363_00945) pilA 180559..181011 (+) 453 WP_010787008.1 pilin Machinery gene
  C3363_RS00930 (C3363_00950) - 181284..181649 (+) 366 WP_015940027.1 endonuclease domain-containing protein -
  C3363_RS00935 (C3363_00955) pilB 182002..183387 (+) 1386 WP_010787006.1 GspE/PulE family protein Machinery gene
  C3363_RS00940 (C3363_00960) pilC 183380..184576 (+) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  C3363_RS00945 (C3363_00965) - 184573..185241 (+) 669 WP_010787005.1 prepilin peptidase -
  C3363_RS00950 (C3363_00970) dapE 185325..186458 (+) 1134 WP_010787004.1 succinyl-diaminopimelate desuccinylase -
  C3363_RS00955 (C3363_00975) clpX 186532..187782 (-) 1251 WP_005711769.1 ATP-dependent protease ATP-binding subunit ClpX -
  C3363_RS00960 (C3363_00980) clpP 187789..188370 (-) 582 WP_010787003.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52808.65 Da        Isoelectric Point: 5.3881

>NTDB_id=290748 C3363_RS00935 WP_010787006.1 182002..183387(+) (pilB) [Glaesserella parasuis strain GZ20170512]
MSQYTICEQQTERIFDISPSRWQKNCEEKQLLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPIYEESELAFVEQEQEQLNLNDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNRSIQLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALSAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGVGC
DECYQGYRGRIGVYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNETEVDRVLGYE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=290748 C3363_RS00935 WP_010787006.1 182002..183387(+) (pilB) [Glaesserella parasuis strain GZ20170512]
ATGTCCCAATACACTATCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAAAAAAATTGCGA
GGAGAAACAGCTTTTATTGCGTTACCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCAGTGGTGATTGCTTCTGATGAA
CTGAAATACTTGCTTAACGCTCTCTCGCCTGAACAACAGCCCATTTATGAAGAGAGCGAGCTGGCTTTTGTTGAACAGGA
GCAAGAACAGCTCAACCTAAACGATCCGATTATTCAACTGCTAGATAATCTCTTTAAATTTTGTCTAGCTCAGAATGCGT
CCGACATTCATATTGAGCCACGCAAGCAAAAATTGATTATCCGCTTACGCATTGACGGCGTTTTGCACCTGTATAAAAGC
CTATCTATTCAGCTGGCAAGCCGACTAATTTCACGGATAAAACTACTGGCAAAGCTAGATATTAGCGAATTACGCCAGCC
ACAAGACGGGCAATTTAGTTTTACCACCGCCCTTGCGGATACCCTTGATTTTCGGGTGTCTAGCCTGCCGACGATCTACG
GTGAAAAACTGGTATTACGCTTACAAAAAAACAAGCCGACCAGTTTTGATTTTCTACAACTTGGCTTTAATCCACAACAG
CAAACCACACTGCTCAACGCTCTGAAACAACCACAGGGGCTGATTTTAGTGACCGGACCAACGGGCAGTGGCAAAAGCAT
CACCCTGTACAGTGCCTTAAACTATCTTAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCGGTGGAAATCGAGA
TTGACGGCTTAATTCAAACCCAAGTTAATCGCAGTATTCAACTGGATTTTAGCCAGCTGTTACGCACCTTTCTACGCCAA
GATCCTGACATCATTATGTTAGGCGAAATTCGAGATGAAGAGAGTGCCAAAATGGCGTTACGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCGACCTTGCATACCAACGACGCACTCTCTGCGGTCGAACGCTTGTTGCAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTGATTGCACAACGCCTTGTGCGTAAGCTCTGTAAAAAGTGTCGTGGAGTAGGCTGT
GACGAGTGCTATCAAGGCTATAGAGGGCGGATCGGGGTTTACCAATGTTTAAGCAGAACGGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGATTTTTCCAGCCTACGAGAGAGTGCTAAACAGAAAATTGAACAACAACTCACCAATGAAACCGAAG
TGGATAGGGTATTAGGTTATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

100

100

1
  pilB Haemophilus influenzae Rd KW20

58.093

97.831

0.568
  pilB Haemophilus influenzae 86-028NP

57.456

98.915

0.568
  pilB Vibrio cholerae strain A1552

38.2

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.13

100

0.41
  pilB Legionella pneumophila strain ERS1305867

38.211

100

0.408
  pilB Vibrio campbellii strain DS40M4

38.923

100

0.408
  pilB Acinetobacter baumannii D1279779

36.187

100

0.403
  pilB Acinetobacter baylyi ADP1

36.634

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.248

100

0.388
  pilF Neisseria gonorrhoeae MS11

36.421

100

0.375
  pilF Thermus thermophilus HB27

35.01

100

0.362

Multiple sequence alignment