Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   CSC50_RS00175 Genome accession   NZ_CP029082
Coordinates   695..1123 (-) Length   142 a.a.
NCBI ID   WP_000934385.1    Uniprot ID   A0A2K0CLZ8
Organism   Staphylococcus aureus strain AR465     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 7..9019 695..1123 within 0


Gene organization within MGE regions


Location: 7..9019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSC50_RS00170 (CSC50_0001) - 7..681 (-) 675 WP_001124438.1 putative HNHc nuclease -
  CSC50_RS00175 (CSC50_0002) ssbA 695..1123 (-) 429 WP_000934385.1 single-stranded DNA-binding protein Machinery gene
  CSC50_RS00180 (CSC50_0003) - 1123..1746 (-) 624 WP_000139720.1 DUF1071 domain-containing protein -
  CSC50_RS00185 (CSC50_0004) - 1739..1960 (-) 222 WP_000815401.1 DUF2483 family protein -
  CSC50_RS00190 (CSC50_0005) - 1970..2230 (-) 261 WP_000291090.1 DUF1108 family protein -
  CSC50_RS00195 (CSC50_0006) - 2235..2537 (-) 303 WP_000165363.1 DUF2482 family protein -
  CSC50_RS00200 - 2631..2792 (-) 162 WP_000066011.1 DUF1270 domain-containing protein -
  CSC50_RS00205 (CSC50_0007) - 2785..3006 (-) 222 WP_000977381.1 hypothetical protein -
  CSC50_RS00210 (CSC50_0008) - 3020..3469 (-) 450 WP_001094943.1 hypothetical protein -
  CSC50_RS00215 (CSC50_0009) - 3509..3733 (-) 225 WP_000187184.1 hypothetical protein -
  CSC50_RS00220 (CSC50_0010) - 3734..4501 (-) 768 WP_001002757.1 phage antirepressor Ant -
  CSC50_RS00225 (CSC50_0011) - 4501..4695 (-) 195 WP_000108122.1 helix-turn-helix transcriptional regulator -
  CSC50_RS00230 (CSC50_0012) - 4958..5290 (+) 333 WP_001055143.1 helix-turn-helix transcriptional regulator -
  CSC50_RS00235 (CSC50_0013) - 5307..5981 (+) 675 WP_000775187.1 ImmA/IrrE family metallo-endopeptidase -
  CSC50_RS00240 (CSC50_0014) - 6009..6734 (+) 726 WP_000661437.1 PH domain-containing protein -
  CSC50_RS00245 (CSC50_0015) - 6766..7698 (+) 933 WP_000392186.1 hypothetical protein -
  CSC50_RS00250 (CSC50_0016) - 7678..7857 (-) 180 WP_000337826.1 hypothetical protein -
  CSC50_RS00255 (CSC50_0017) - 7970..9019 (+) 1050 WP_001145726.1 tyrosine-type recombinase/integrase -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 15872.58 Da        Isoelectric Point: 8.4825

>NTDB_id=290154 CSC50_RS00175 WP_000934385.1 695..1123(-) (ssbA) [Staphylococcus aureus strain AR465]
MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGEREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTR
SYENKVGQRVFVTEVVADSVQFLEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF

Nucleotide


Download         Length: 429 bp        

>NTDB_id=290154 CSC50_RS00175 WP_000934385.1 695..1123(-) (ssbA) [Staphylococcus aureus strain AR465]
ATGTTAAACAGAGCAGTATTAGTAGGACGCTTAACAAAAGACCCAGAATTAAGAAGCGCGCCAAATGGCGTAAATGTAGG
TACATTCACATTGGCAGTAAACAGAACATTCACGAATGCTCAAGGCGAGCGTGAAGCAGATTTTATAAACGTAGTAGTGT
TCAAGAAACAAGCTGAAAATGTTAAAAACTACCTTTCTAAAGGGTCGCTGGCAGGTGTAGACGGGCGACTACAAACACGT
AGCTACGAAAATAAAGTCGGGCAACGTGTATTTGTGACAGAAGTAGTAGCGGACAGTGTTCAATTCTTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAAGACAAACTCAAACTGGTAATAATCCTTTTGATAATACCACTG
CGATTACTGATGATGACTTACCGTTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2K0CLZ8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

58.14

100

0.704

  ssb Latilactobacillus sakei subsp. sakei 23K

50.588

100

0.606

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

74.648

0.437

  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.958

100

0.423

  ssbA Streptococcus mutans UA159

40.845

100

0.408

  ssbB/cilA Streptococcus mitis NCTC 12261

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae Rx1

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae D39

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae R6

39.437

100

0.394

  ssbB/cilA Streptococcus mitis SK321

39.437

100

0.394


Multiple sequence alignment