Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DA456_RS14795 Genome accession   NZ_CP028490
Coordinates   3408746..3409963 (+) Length   405 a.a.
NCBI ID   WP_011266635.1    Uniprot ID   A0AAD0IB66
Organism   Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3403746..3414963
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA456_RS14775 (DA456_14775) - 3404566..3405564 (-) 999 WP_057419753.1 ATP-grasp domain-containing protein -
  DA456_RS14780 (DA456_14780) - 3405684..3406229 (+) 546 WP_003345116.1 DUF1523 family protein -
  DA456_RS14785 (DA456_14785) - 3406404..3406823 (-) 420 WP_016567160.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DA456_RS14790 (DA456_14790) pilB 3407049..3408743 (+) 1695 WP_003345119.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DA456_RS14795 (DA456_14795) pilC 3408746..3409963 (+) 1218 WP_011266635.1 type II secretion system F family protein Machinery gene
  DA456_RS14800 (DA456_14800) pilD 3409965..3410837 (+) 873 WP_029572867.1 A24 family peptidase Machinery gene
  DA456_RS14805 (DA456_14805) coaE 3410834..3411457 (+) 624 WP_029572866.1 dephospho-CoA kinase -
  DA456_RS14810 (DA456_14810) yacG 3411454..3411663 (+) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  DA456_RS14815 (DA456_14815) - 3411790..3412251 (-) 462 WP_029573726.1 hypothetical protein -
  DA456_RS14820 (DA456_14820) - 3412378..3413007 (+) 630 WP_003392710.1 DUF1780 domain-containing protein -
  DA456_RS14825 (DA456_14825) - 3413004..3413537 (+) 534 WP_003317783.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44124.10 Da        Isoelectric Point: 10.1956

>NTDB_id=285302 DA456_RS14795 WP_011266635.1 3408746..3409963(+) (pilC) [Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)]
MASKAAKVIVYTWEGVDKKGTKTSGELSGHNLALVKAQLRKQGINPTKVRKKSVSIFGKGKKIKPLDIAFFSRQMATMMK
AGVPLLQSFDIISEGAENPNMRALVGSLKQEVSAGNSFATALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPIAVLIVALIVSGILLIKVVPQFQSVFASFGAQLPTFTLMVIGLSDVVQKWWLAIVGLFFVSFFIFK
RAYKQSQKFRDSLDRLLLKVPIIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFKNAVIKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDTMLDKVATYYEDEVDNMVDNLTSLMEPMIMAFLGVIVGGLVIAMYLPIFQ
LGNVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=285302 DA456_RS14795 WP_011266635.1 3408746..3409963(+) (pilC) [Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)]
ATGGCCAGCAAGGCAGCAAAAGTCATCGTTTACACTTGGGAAGGTGTTGACAAGAAAGGTACTAAAACCAGTGGGGAACT
GAGTGGCCATAACTTGGCCCTTGTAAAAGCCCAGCTGCGCAAGCAAGGCATCAACCCAACCAAGGTACGCAAAAAATCAG
TTTCGATCTTTGGCAAGGGCAAGAAGATCAAACCGCTGGACATTGCGTTCTTTTCTCGCCAGATGGCTACGATGATGAAG
GCCGGTGTGCCATTGCTGCAATCGTTTGACATCATTAGCGAAGGCGCTGAGAACCCTAATATGCGCGCACTGGTTGGCTC
GCTAAAGCAGGAAGTTTCGGCAGGTAATAGTTTTGCGACGGCTCTGCGACAAAAACCCGAATATTTCGATGATCTGTTTT
GTAACCTAGTAGATGCCGGCGAGCAAGCTGGCGCACTTGAAAGCCTGCTGGACCGTGTAGCCAGCTATAAAGAGAAGACC
GAAAAGCTAAAAGCTAAAATCAAGAAAGCCATGACATACCCGATTGCCGTATTGATTGTTGCATTGATTGTATCCGGCAT
TCTGCTGATCAAAGTGGTGCCGCAGTTCCAGTCAGTCTTCGCTAGCTTTGGCGCTCAGTTACCGACATTTACCCTGATGG
TTATTGGTCTATCGGACGTCGTACAAAAATGGTGGCTGGCCATTGTCGGGCTATTTTTCGTCAGCTTTTTTATATTTAAA
CGTGCTTACAAACAGTCTCAAAAGTTTCGAGATAGCCTCGACCGACTTCTGCTGAAAGTGCCCATCATTGGGCCATTGAT
TTTCAAATCTTCGGTGGCACGCTACGCGCGCACGCTTGCAACAACGTTTGCCGCTGGCGTCCCGCTTGTGGAGGCTTTGG
ACTCAGTCGCCGGAGCGACTGGCAATGTAGTATTTAAAAATGCAGTCATCAAGGTAAAACAGGACGTCTCCACGGGTATG
CAACTGAATTTCTCGATGCGTTCAACAGGAGTGTTTCCGAGCTTGGCGATTCAGATGACAGCCATTGGCGAAGAGTCCGG
CGCGCTGGACACCATGCTCGACAAGGTAGCTACCTATTACGAGGACGAAGTCGATAACATGGTCGACAATCTGACCAGCC
TTATGGAACCGATGATCATGGCCTTTTTGGGCGTGATAGTTGGCGGGCTGGTAATTGCGATGTATCTACCTATCTTCCAA
CTCGGAAACGTCGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

59.804

100

0.602
  pilC Acinetobacter baumannii D1279779

59.559

100

0.6
  pilC Legionella pneumophila strain ERS1305867

50.985

100

0.511
  pilC Vibrio campbellii strain DS40M4

42.998

100

0.432
  pilG Neisseria gonorrhoeae MS11

42.821

98.025

0.42
  pilC Vibrio cholerae strain A1552

42.677

97.778

0.417
  pilG Neisseria meningitidis 44/76-A

42.569

98.025

0.417
  pilC Thermus thermophilus HB27

39.401

99.012

0.39

Multiple sequence alignment