Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DA456_RS14790 Genome accession   NZ_CP028490
Coordinates   3407049..3408743 (+) Length   564 a.a.
NCBI ID   WP_003345119.1    Uniprot ID   A0AAD0IA27
Organism   Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3402049..3413743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DA456_RS14765 (DA456_14765) - 3402172..3403305 (-) 1134 WP_004415102.1 phosphoribosyltransferase domain-containing protein -
  DA456_RS14770 (DA456_14770) - 3403372..3404511 (-) 1140 WP_057419752.1 trypsin-like peptidase domain-containing protein -
  DA456_RS14775 (DA456_14775) - 3404566..3405564 (-) 999 WP_057419753.1 ATP-grasp domain-containing protein -
  DA456_RS14780 (DA456_14780) - 3405684..3406229 (+) 546 WP_003345116.1 DUF1523 family protein -
  DA456_RS14785 (DA456_14785) - 3406404..3406823 (-) 420 WP_016567160.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DA456_RS14790 (DA456_14790) pilB 3407049..3408743 (+) 1695 WP_003345119.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DA456_RS14795 (DA456_14795) pilC 3408746..3409963 (+) 1218 WP_011266635.1 type II secretion system F family protein Machinery gene
  DA456_RS14800 (DA456_14800) pilD 3409965..3410837 (+) 873 WP_029572867.1 A24 family peptidase Machinery gene
  DA456_RS14805 (DA456_14805) coaE 3410834..3411457 (+) 624 WP_029572866.1 dephospho-CoA kinase -
  DA456_RS14810 (DA456_14810) yacG 3411454..3411663 (+) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  DA456_RS14815 (DA456_14815) - 3411790..3412251 (-) 462 WP_029573726.1 hypothetical protein -
  DA456_RS14820 (DA456_14820) - 3412378..3413007 (+) 630 WP_003392710.1 DUF1780 domain-containing protein -
  DA456_RS14825 (DA456_14825) - 3413004..3413537 (+) 534 WP_003317783.1 MOSC domain-containing protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62539.99 Da        Isoelectric Point: 6.4534

>NTDB_id=285301 DA456_RS14790 WP_003345119.1 3407049..3408743(+) (pilB) [Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)]
MTDAVLTGLAKQLVLAELLTEPVAQQAYQQARRDKISLVSYLVQHKLVKSLTLAELASDQFGVPFMDLASLDKESLPKGL
VSEKLVRQHHALPLWRRGNKLFIGISDPTNHQAVTDIQFSTGLNTEAILVEDDKLTVAIDRFFDSDSGLGNLEDVDLGLD
IEPADGKETTLATQNDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKIFRVRLRTDGILHEIARPPIHLANRIAARLKV
MASLDISERRKPQDGRVKLRVSKTKAIDFRMNTLPTLWGEKIVMRILDPTSAQMGIDALGYEPEQKALYLEALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCSHCKKELEIPRETLIKEGFPEAK
IGTFKIYGPMGCEHCNGGYRGRVGIYEVVKKTPELERIIMEEGNSLEISRQMRKDGFNDLRTSGLSKAMQGITSLEEVNR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=285301 DA456_RS14790 WP_003345119.1 3407049..3408743(+) (pilB) [Pseudomonas syringae pv. atrofaciens strain LMG5095 isolate Triticum aestivum (Aestivum Group)]
ATGACTGATGCTGTCCTCACTGGCCTGGCCAAACAATTGGTCCTAGCCGAGCTACTTACTGAACCTGTTGCGCAGCAAGC
GTATCAGCAGGCGCGCCGCGACAAGATTTCGTTGGTGAGCTATCTGGTTCAGCACAAACTGGTCAAAAGCCTGACGCTGG
CAGAATTGGCCTCCGATCAGTTCGGCGTGCCGTTCATGGACCTCGCCAGCCTGGACAAGGAAAGCCTGCCTAAAGGCCTG
GTGAGTGAAAAGCTGGTTCGACAGCATCACGCCCTGCCGCTTTGGCGACGCGGTAATAAGCTATTTATCGGGATTTCCGA
CCCTACGAATCATCAGGCGGTGACTGATATCCAGTTCAGCACTGGCCTGAACACCGAAGCGATTCTGGTTGAAGATGACA
AGCTGACTGTTGCGATAGACCGTTTTTTTGACAGCGATAGCGGTCTGGGCAATCTGGAGGATGTCGATCTGGGCCTGGAT
ATCGAACCGGCCGATGGCAAGGAAACCACGCTAGCTACACAGAATGACGCTGACGATGCTCCGGTGGTGAGATTCGTCAA
CAAGATGCTTATGGATGCCATCCGACTAGGATCATCCGACTTGCACTTTGAGCCCTATGAAAAGATCTTCCGCGTACGTT
TGCGTACCGATGGCATCCTGCACGAAATCGCCAGACCGCCGATCCATTTGGCAAACCGTATTGCTGCGCGTCTGAAGGTC
ATGGCCAGCCTCGATATTTCGGAACGACGCAAACCGCAGGACGGAAGGGTCAAGTTGCGGGTGTCCAAAACAAAGGCCAT
CGATTTTCGTATGAATACCTTGCCCACACTGTGGGGCGAAAAGATCGTGATGCGGATCCTCGACCCGACCAGCGCTCAGA
TGGGTATCGATGCGCTGGGCTATGAGCCAGAACAAAAGGCTTTGTATCTGGAGGCGTTGAAACAGCCGCAAGGCATGATT
CTTGTCACCGGCCCTACGGGTTCCGGCAAGACAGTTTCGCTGTATACGGGTCTGAATATTCTCAACACCGTGGACATCAA
CATATCCACAGCCGAAGACCCGGTAGAGATCAACCTGGAAGGTATCAATCAGGTCAACGTCAACCCGCGCCAAGGGCTGG
ACTTTTCTCAGGCATTGCGCGCCTTCCTGCGTCAAGATCCCGACGTAATTATGGTCGGCGAGATACGCGACCTGGAAACC
GCCGAAATCGCAATTAAGGCGTCGCAAACCGGCCACATGGTGCTTTCAACGCTGCACACCAACAGCGCAGCAGAAACATT
GACTCGCTTGCACCATATGGGTGTTGCAGCATTCAACATTGCCACGGCAATAAACCTGATTATTGCGCAACGACTGGCAC
GTAAACTGTGTAGCCACTGCAAGAAAGAACTCGAGATTCCTCGCGAGACACTGATCAAAGAAGGCTTTCCAGAGGCGAAG
ATCGGTACGTTCAAGATCTATGGGCCGATGGGCTGCGAGCACTGCAACGGGGGTTACCGAGGCAGGGTGGGCATTTACGA
AGTGGTCAAGAAAACACCGGAACTGGAACGCATCATCATGGAAGAAGGCAACTCGCTGGAGATCTCCAGACAGATGCGCA
AGGACGGTTTCAATGACCTGCGAACCTCAGGCCTGTCAAAAGCTATGCAGGGCATTACCAGCCTTGAAGAAGTCAACCGT
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.672

100

0.58
  pilB Acinetobacter baylyi ADP1

56.537

100

0.567
  pilB Legionella pneumophila strain ERS1305867

54.657

100

0.551
  pilB Vibrio cholerae strain A1552

50.712

99.645

0.505
  pilB Vibrio parahaemolyticus RIMD 2210633

50.626

99.113

0.502
  pilB Vibrio campbellii strain DS40M4

49.823

100

0.5
  pilF Neisseria gonorrhoeae MS11

48.307

99.468

0.481
  pilF Thermus thermophilus HB27

39.046

100

0.392
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.279

93.44

0.367

Multiple sequence alignment