Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB/cilA   Type   Machinery gene
Locus tag   SK637_RS02045 Genome accession   NZ_CP028415
Coordinates   384865..385260 (+) Length   131 a.a.
NCBI ID   WP_033688178.1    Uniprot ID   A0A1T0C5A0
Organism   Streptococcus mitis strain SK637     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 381405..402301 384865..385260 within 0


Gene organization within MGE regions


Location: 381405..402301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SK637_RS02020 (SK637_00394) - 381405..382478 (+) 1074 WP_033688172.1 N-acetylmuramoyl-L-alanine amidase family protein -
  SK637_RS10220 (SK637_00395) - 382503..382634 (-) 132 WP_033688173.1 hypothetical protein -
  SK637_RS02025 (SK637_00396) - 382728..383027 (+) 300 WP_033688174.1 DUF4651 domain-containing protein -
  SK637_RS02030 (SK637_00397) - 383024..383341 (+) 318 WP_000615777.1 thioredoxin family protein -
  SK637_RS02035 (SK637_00398) ytpR 383357..383983 (+) 627 WP_033688176.1 YtpR family tRNA-binding protein -
  SK637_RS02040 (SK637_00399) - 384026..384787 (+) 762 WP_033688177.1 SDR family NAD(P)-dependent oxidoreductase -
  SK637_RS02045 (SK637_00400) ssbB/cilA 384865..385260 (+) 396 WP_033688178.1 single-stranded DNA-binding protein Machinery gene
  SK637_RS02050 (SK637_00401) groES 385416..385700 (+) 285 WP_033688179.1 co-chaperone GroES -
  SK637_RS02055 (SK637_00402) groL 385716..387338 (+) 1623 WP_033688181.1 chaperonin GroEL -
  SK637_RS02060 (SK637_00403) - 387476..388243 (+) 768 WP_000567567.1 epoxyqueuosine reductase QueH -
  SK637_RS02065 (SK637_00404) - 388466..388999 (-) 534 WP_000775316.1 DUF402 domain-containing protein -
  SK637_RS02070 (SK637_00405) recX 389088..389864 (-) 777 WP_033688183.1 recombination regulator RecX -
  SK637_RS02075 (SK637_00406) - 389954..391120 (-) 1167 WP_033688184.1 tyrosine-type recombinase/integrase -
  SK637_RS02085 (SK637_00407) - 391529..392671 (-) 1143 WP_050489866.1 Fic family protein -
  SK637_RS02090 (SK637_00408) - 393091..393630 (-) 540 WP_033688188.1 hypothetical protein -
  SK637_RS02095 (SK637_00409) - 393678..393947 (-) 270 WP_000505375.1 DUF4177 domain-containing protein -
  SK637_RS02100 (SK637_00410) - 393987..394508 (-) 522 WP_033688189.1 helix-turn-helix domain-containing protein -
  SK637_RS02105 (SK637_00411) - 394710..394907 (+) 198 WP_000794673.1 helix-turn-helix domain-containing protein -
  SK637_RS02110 (SK637_00412) - 395138..395740 (+) 603 WP_033688200.1 Rha family transcriptional regulator -
  SK637_RS02115 (SK637_00413) - 395752..396012 (+) 261 WP_033688190.1 hypothetical protein -
  SK637_RS02125 (SK637_00414) - 396333..396638 (+) 306 WP_237397639.1 hypothetical protein -
  SK637_RS02130 (SK637_00415) - 396595..396798 (+) 204 WP_033688191.1 hypothetical protein -
  SK637_RS10055 - 396800..397006 (+) 207 WP_078352928.1 hypothetical protein -
  SK637_RS02140 (SK637_00416) - 397011..397283 (+) 273 WP_033688202.1 hypothetical protein -
  SK637_RS02145 (SK637_00417) - 397270..398154 (+) 885 WP_033688192.1 conserved phage C-terminal domain-containing protein -
  SK637_RS02150 - 398166..398988 (+) 823 Protein_377 ATP-binding protein -
  SK637_RS09835 (SK637_00419) - 398994..399149 (+) 156 WP_000344069.1 hypothetical protein -
  SK637_RS02155 (SK637_00420) - 399239..399433 (+) 195 WP_000090304.1 hypothetical protein -
  SK637_RS02160 (SK637_00421) - 399517..400377 (+) 861 WP_033688194.1 phage major capsid protein -
  SK637_RS02165 (SK637_00422) - 400387..400938 (+) 552 WP_033688195.1 hypothetical protein -
  SK637_RS02170 (SK637_00423) - 401364..401711 (+) 348 WP_237397640.1 DUF722 domain-containing protein -
  SK637_RS02175 - 401966..402301 (+) 336 WP_033688196.1 YjcQ family protein -

Sequence


Protein


Download         Length: 131 a.a.        Molecular weight: 14879.80 Da        Isoelectric Point: 5.9409

>NTDB_id=284695 SK637_RS02045 WP_033688178.1 384865..385260(+) (ssbB/cilA) [Streptococcus mitis strain SK637]
MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREADFVNMVLWGRLAETLASYATKGSLISADGELRTR
RFEKNGQMNYVTEVLVTGFQLLESRAQRAMRENNAGQDLADLVLEEEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=284695 SK637_RS02045 WP_033688178.1 384865..385260(+) (ssbB/cilA) [Streptococcus mitis strain SK637]
ATGTATAATAAAGTTATCTTAATCGGACGCTTGACGTCTACACCAGAATTGCACAAAACCAACAATGACAAGTCAGTAGC
GCGTGCGACTATTGCAGTCAACCGTCGTTACAAAGACCAAAATGGGGAACGCGAAGCTGACTTTGTCAATATGGTCCTAT
GGGGCAGACTAGCAGAAACCTTGGCAAGCTACGCAACAAAAGGAAGTCTCATTTCTGCGGACGGAGAACTTCGTACCCGT
CGTTTTGAGAAAAATGGTCAGATGAACTACGTTACCGAAGTACTTGTTACAGGATTCCAACTCTTGGAAAGTCGTGCCCA
ACGTGCCATGCGTGAAAATAATGCGGGGCAAGACTTAGCGGACTTGGTTTTGGAAGAGGAAGAATTGCCATTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A1T0C5A0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB/cilA Streptococcus pneumoniae TIGR4

99.237

100

0.992

  ssbB/cilA Streptococcus pneumoniae Rx1

98.473

100

0.985

  ssbB/cilA Streptococcus pneumoniae D39

98.473

100

0.985

  ssbB/cilA Streptococcus pneumoniae R6

98.473

100

0.985

  ssbB/cilA Streptococcus mitis SK321

98.473

100

0.985

  ssbB/cilA Streptococcus mitis NCTC 12261

96.947

100

0.969

  ssbA Streptococcus mutans UA159

75.573

100

0.756

  ssbB Streptococcus sobrinus strain NIDR 6715-7

70.992

100

0.71

  ssbB Lactococcus lactis subsp. cremoris KW2

59.821

85.496

0.511

  ssbA Bacillus subtilis subsp. subtilis str. 168

50.943

80.916

0.412

  ssb Latilactobacillus sakei subsp. sakei 23K

46.903

86.26

0.405


Multiple sequence alignment