Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   EEL45_RS24260 Genome accession   NZ_CP033605
Coordinates   4656497..4657165 (-) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli O157:H7 strain TR01     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 4651497..4662165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EEL45_RS24230 (EEL45_24230) rfaQ 4651675..4652733 (-) 1059 WP_000360402.1 lipopolysaccharide core heptosyltransferase RfaQ -
  EEL45_RS24235 (EEL45_24235) waaA 4653146..4654423 (+) 1278 WP_123178715.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  EEL45_RS24240 (EEL45_24240) coaD 4654431..4654910 (+) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  EEL45_RS24245 (EEL45_24245) mutM 4654949..4655758 (-) 810 WP_123178716.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  EEL45_RS24250 (EEL45_24250) rpmG 4655856..4656023 (-) 168 WP_001051798.1 50S ribosomal protein L33 -
  EEL45_RS24255 (EEL45_24255) rpmB 4656044..4656280 (-) 237 WP_000091955.1 50S ribosomal protein L28 -
  EEL45_RS24260 (EEL45_24260) radC 4656497..4657165 (-) 669 WP_001297375.1 DNA repair protein RadC Machinery gene
  EEL45_RS24265 (EEL45_24265) coaBC 4657337..4658557 (+) 1221 WP_032187724.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  EEL45_RS24270 (EEL45_24270) dut 4658535..4658993 (+) 459 WP_000976070.1 dUTP diphosphatase -
  EEL45_RS24275 (EEL45_24275) slmA 4659100..4659696 (+) 597 WP_000818601.1 nucleoid occlusion factor SlmA -
  EEL45_RS24280 (EEL45_24280) pyrE 4659733..4660374 (-) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  EEL45_RS24285 (EEL45_24285) rph 4660440..4661156 (-) 717 WP_001247089.1 ribonuclease PH -
  EEL45_RS24290 (EEL45_24290) yicC 4661283..4662146 (+) 864 WP_000621326.1 YicC/YloC family endoribonuclease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=283705 EEL45_RS24260 WP_001297375.1 4656497..4657165(-) (radC) [Escherichia coli O157:H7 strain TR01]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=283705 EEL45_RS24260 WP_001297375.1 4656497..4657165(-) (radC) [Escherichia coli O157:H7 strain TR01]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GCTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAACAATTTAGTGGTGTTCATGGAATTGGCGTGGCGAAATTTGCCCAGTTA
AAGGGGATTGCTGAACTGGCGCGGCGTTACTACAACGTGCGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGTGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCACAACACCGGG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCACATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment