Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   LL14B4_RS12770 Genome accession   NZ_CP028160
Coordinates   2557301..2557726 (-) Length   141 a.a.
NCBI ID   WP_109991390.1    Uniprot ID   A0A2Z3KHN3
Organism   Lactococcus lactis subsp. lactis strain 14B4     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 2529248..2565535 2557301..2557726 within 0


Gene organization within MGE regions


Location: 2529248..2565535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LL14B4_RS12575 (LL14B4_12565) - 2529248..2530246 (+) 999 WP_109991354.1 IS30 family transposase -
  LL14B4_RS12580 (LL14B4_12570) - 2530427..2530963 (+) 537 WP_109991355.1 hypothetical protein -
  LL14B4_RS12585 (LL14B4_12575) - 2531062..2531814 (-) 753 WP_109991356.1 Panacea domain-containing protein -
  LL14B4_RS12590 (LL14B4_12580) - 2531818..2532462 (-) 645 WP_109991357.1 hypothetical protein -
  LL14B4_RS12595 (LL14B4_12585) - 2532735..2533160 (-) 426 WP_109991358.1 DUF1761 domain-containing protein -
  LL14B4_RS12600 (LL14B4_12590) - 2533244..2534584 (-) 1341 WP_109991359.1 LysM peptidoglycan-binding domain-containing protein -
  LL14B4_RS12605 (LL14B4_12595) - 2534581..2534931 (-) 351 WP_109991360.1 holin -
  LL14B4_RS12610 (LL14B4_12600) - 2534980..2536266 (-) 1287 WP_109991361.1 pyocin knob domain-containing protein -
  LL14B4_RS12615 (LL14B4_12605) - 2536276..2537394 (-) 1119 WP_109991362.1 siphovirus ReqiPepy6 Gp37-like family protein -
  LL14B4_RS12620 (LL14B4_12610) - 2537396..2538247 (-) 852 WP_109991363.1 phage distal tail protein -
  LL14B4_RS12625 (LL14B4_12615) - 2538260..2541739 (-) 3480 WP_109991364.1 phage tail tape measure protein -
  LL14B4_RS12630 (LL14B4_12620) - 2541739..2542311 (-) 573 WP_109991365.1 Gp15 family bacteriophage protein -
  LL14B4_RS12635 (LL14B4_12625) - 2542324..2542797 (-) 474 WP_109991366.1 hypothetical protein -
  LL14B4_RS12640 (LL14B4_12630) - 2542855..2543124 (-) 270 WP_338067065.1 fibronectin type III domain-containing protein -
  LL14B4_RS12645 (LL14B4_12635) - 2543198..2543680 (-) 483 WP_109991368.1 phage tail tube protein -
  LL14B4_RS12650 (LL14B4_12640) - 2543673..2544086 (-) 414 WP_109991369.1 minor capsid protein -
  LL14B4_RS12655 (LL14B4_12645) - 2544076..2544447 (-) 372 WP_109991370.1 minor capsid protein -
  LL14B4_RS12660 (LL14B4_12650) - 2544447..2544794 (-) 348 WP_109991371.1 putative minor capsid protein -
  LL14B4_RS12665 (LL14B4_12655) - 2544794..2545198 (-) 405 WP_109991372.1 hypothetical protein -
  LL14B4_RS12670 (LL14B4_12660) - 2545225..2545473 (-) 249 WP_109991373.1 Ig-like domain-containing protein -
  LL14B4_RS12675 (LL14B4_12665) - 2545491..2546444 (-) 954 WP_109991374.1 phage major capsid protein -
  LL14B4_RS12680 (LL14B4_12670) - 2546455..2547033 (-) 579 WP_109991375.1 phage scaffolding protein -
  LL14B4_RS12690 (LL14B4_12680) - 2547222..2547470 (-) 249 WP_031558864.1 DUF6275 family protein -
  LL14B4_RS12695 (LL14B4_12685) - 2547520..2548719 (-) 1200 WP_239449814.1 phage minor capsid protein -
  LL14B4_RS12700 (LL14B4_12690) - 2548722..2550266 (-) 1545 WP_109991377.1 phage portal protein -
  LL14B4_RS12705 (LL14B4_12695) - 2550268..2551473 (-) 1206 WP_162551491.1 PBSX family phage terminase large subunit -
  LL14B4_RS12710 (LL14B4_12700) - 2551466..2551861 (-) 396 WP_109991379.1 phBC6A51 family helix-turn-helix protein -
  LL14B4_RS12715 (LL14B4_12705) - 2552036..2552494 (-) 459 WP_109991380.1 hypothetical protein -
  LL14B4_RS12720 (LL14B4_12710) - 2552504..2553304 (-) 801 WP_109991381.1 hypothetical protein -
  LL14B4_RS12725 (LL14B4_12715) - 2553308..2553859 (-) 552 WP_109991382.1 LemA family protein -
  LL14B4_RS13610 - 2553980..2554333 (-) 354 WP_239449824.1 DUF1642 domain-containing protein -
  LL14B4_RS12735 (LL14B4_12725) - 2554326..2554601 (-) 276 WP_109991383.1 hypothetical protein -
  LL14B4_RS12740 (LL14B4_12730) - 2554598..2554804 (-) 207 WP_109991384.1 hypothetical protein -
  LL14B4_RS12745 (LL14B4_12735) - 2554822..2555010 (-) 189 WP_109991385.1 hypothetical protein -
  LL14B4_RS12750 (LL14B4_12740) - 2555007..2555432 (-) 426 WP_109991386.1 hypothetical protein -
  LL14B4_RS12755 (LL14B4_12745) - 2555492..2555863 (+) 372 WP_109991387.1 hypothetical protein -
  LL14B4_RS13365 - 2555793..2556002 (-) 210 WP_162551492.1 hypothetical protein -
  LL14B4_RS12760 (LL14B4_12750) - 2556018..2556260 (-) 243 WP_109991388.1 hypothetical protein -
  LL14B4_RS12765 (LL14B4_12755) - 2556253..2557176 (-) 924 WP_109991389.1 phage replisome organizer N-terminal domain-containing protein -
  LL14B4_RS12770 (LL14B4_12760) ssb 2557301..2557726 (-) 426 WP_109991390.1 single-stranded DNA-binding protein Machinery gene
  LL14B4_RS12775 (LL14B4_12765) - 2557719..2558792 (-) 1074 WP_109991391.1 DUF1351 domain-containing protein -
  LL14B4_RS12780 (LL14B4_12770) bet 2558794..2559531 (-) 738 WP_109991392.1 phage recombination protein Bet -
  LL14B4_RS12785 (LL14B4_12775) - 2559635..2559871 (-) 237 WP_109991393.1 DUF1408 domain-containing protein -
  LL14B4_RS12790 (LL14B4_12780) - 2559871..2560068 (-) 198 WP_109991394.1 helix-turn-helix domain-containing protein -
  LL14B4_RS12795 (LL14B4_12785) - 2560189..2560470 (+) 282 WP_109991395.1 DUF3892 domain-containing protein -
  LL14B4_RS12800 (LL14B4_12790) - 2560500..2560691 (-) 192 WP_109991396.1 hypothetical protein -
  LL14B4_RS12805 (LL14B4_12795) - 2560834..2561079 (-) 246 WP_109991397.1 hypothetical protein -
  LL14B4_RS12810 (LL14B4_12800) - 2561092..2561844 (-) 753 WP_109991398.1 Rha family transcriptional regulator -
  LL14B4_RS12815 (LL14B4_12805) - 2561863..2562093 (-) 231 WP_015966797.1 helix-turn-helix transcriptional regulator -
  LL14B4_RS12820 (LL14B4_12810) - 2562274..2563035 (+) 762 WP_109991399.1 hypothetical protein -
  LL14B4_RS12825 (LL14B4_12815) - 2563267..2564004 (+) 738 WP_204162922.1 XRE family transcriptional regulator -
  LL14B4_RS12830 (LL14B4_12820) - 2564016..2564339 (+) 324 WP_109991401.1 hypothetical protein -
  LL14B4_RS12835 (LL14B4_12825) - 2564390..2565535 (+) 1146 WP_109991402.1 tyrosine-type recombinase/integrase -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15694.46 Da        Isoelectric Point: 8.9291

>NTDB_id=281840 LL14B4_RS12770 WP_109991390.1 2557301..2557726(-) (ssb) [Lactococcus lactis subsp. lactis strain 14B4]
MINNVTLVGRITKEPELRYTQQNKAVASFTLAVNRQFKNSNGEREADFINCIIWGKSAENLANWTHKGQLIGVTGSIQTR
NYENKQGQRVYITEVVASNFQVLEKSNQANGERVGKPTANQSNSAPFGSSPKEISDDDMPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=281840 LL14B4_RS12770 WP_109991390.1 2557301..2557726(-) (ssb) [Lactococcus lactis subsp. lactis strain 14B4]
ATGATTAATAATGTCACATTAGTGGGGCGAATCACTAAAGAGCCCGAATTAAGATATACACAACAAAATAAGGCAGTTGC
TTCATTTACTCTTGCAGTTAATCGTCAATTTAAGAATTCCAATGGAGAAAGAGAAGCTGACTTTATCAATTGTATTATTT
GGGGTAAATCAGCCGAAAATTTGGCCAATTGGACTCATAAAGGTCAATTAATTGGAGTTACTGGTAGTATTCAAACTCGC
AACTATGAGAATAAACAAGGGCAACGTGTTTATATTACGGAGGTTGTCGCAAGTAATTTCCAAGTACTTGAAAAAAGTAA
TCAAGCAAATGGAGAGCGAGTAGGTAAACCAACTGCTAATCAATCAAACTCTGCCCCATTCGGCAGTTCTCCTAAGGAAA
TATCTGATGATGATATGCCATTTTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2Z3KHN3

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

51.765

100

0.624

  ssbA Bacillus subtilis subsp. subtilis str. 168

47.674

100

0.582

  ssbB/cilA Streptococcus pneumoniae TIGR4

43.262

100

0.433

  ssbB/cilA Streptococcus mitis NCTC 12261

43.262

100

0.433

  ssbB/cilA Streptococcus pneumoniae Rx1

42.553

100

0.426

  ssbB/cilA Streptococcus pneumoniae D39

42.553

100

0.426

  ssbB/cilA Streptococcus pneumoniae R6

42.553

100

0.426

  ssbB/cilA Streptococcus mitis SK321

42.553

100

0.426

  ssbB Bacillus subtilis subsp. subtilis str. 168

56.731

73.759

0.418

  ssbB Streptococcus sobrinus strain NIDR 6715-7

50.926

76.596

0.39

  ssbB Lactococcus lactis subsp. cremoris KW2

52.427

73.05

0.383


Multiple sequence alignment