Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   C6568_RS10255 Genome accession   NZ_CP027667
Coordinates   2168583..2169023 (-) Length   146 a.a.
NCBI ID   WP_199792737.1    Uniprot ID   -
Organism   Melaminivora suipulveris strain SC2-9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2163583..2174023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6568_RS10240 (C6568_10240) - 2165371..2165988 (+) 618 WP_199792736.1 Uma2 family endonuclease -
  C6568_RS10245 (C6568_10245) - 2166174..2167217 (-) 1044 WP_106684038.1 Rpn family recombination-promoting nuclease/putative transposase -
  C6568_RS10250 (C6568_10250) - 2167360..2168460 (-) 1101 WP_106684039.1 transposase -
  C6568_RS10255 (C6568_10255) pilA2 2168583..2169023 (-) 441 WP_199792737.1 pilin Machinery gene
  C6568_RS18280 (C6568_10260) - 2169551..2169757 (-) 207 WP_106684040.1 BrnA antitoxin family protein -
  C6568_RS10265 (C6568_10265) vapC 2169954..2170370 (-) 417 WP_106684041.1 type II toxin-antitoxin system tRNA(fMet)-specific endonuclease VapC -
  C6568_RS10270 (C6568_10270) vapB 2170370..2170603 (-) 234 WP_106684042.1 type II toxin-antitoxin system VapB family antitoxin -
  C6568_RS10275 (C6568_10275) - 2170785..2171489 (-) 705 WP_106684043.1 FHA domain-containing protein -
  C6568_RS10280 (C6568_10280) - 2171598..2172467 (-) 870 WP_106684044.1 PP2C family protein-serine/threonine phosphatase -
  C6568_RS10285 (C6568_10285) sucD 2172651..2173544 (-) 894 WP_106684045.1 succinate--CoA ligase subunit alpha -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 15073.38 Da        Isoelectric Point: 8.8263

>NTDB_id=278833 C6568_RS10255 WP_199792737.1 2168583..2169023(-) (pilA2) [Melaminivora suipulveris strain SC2-9]
MKRTMRSVQKGFTLIELMIVVAIIGILAAVALPAYQDYTTRAKITEIITAASSCKNSVTEYYQSKGALPADNAAAGCDTQ
ATQYVASLAVANGVVTAATAAAPAIPTAAQGNFVLTPTANAGNAGILDWQCTSSTIAKKFLPANCR

Nucleotide


Download         Length: 441 bp        

>NTDB_id=278833 C6568_RS10255 WP_199792737.1 2168583..2169023(-) (pilA2) [Melaminivora suipulveris strain SC2-9]
ATGAAGCGTACGATGCGTTCCGTACAAAAGGGTTTTACCCTGATCGAACTGATGATCGTGGTGGCGATTATTGGTATTTT
GGCGGCTGTGGCGTTGCCGGCTTATCAGGATTACACTACGCGCGCCAAGATTACTGAAATAATTACGGCGGCAAGCTCGT
GCAAGAACTCCGTAACTGAATATTATCAATCCAAGGGCGCTTTGCCAGCAGATAATGCTGCGGCTGGATGTGATACGCAA
GCTACGCAATATGTAGCTAGCCTGGCAGTAGCAAACGGCGTAGTCACCGCTGCAACAGCAGCGGCTCCTGCAATTCCTAC
TGCTGCTCAAGGCAATTTTGTTTTGACACCGACGGCCAACGCCGGCAACGCTGGAATCCTGGATTGGCAGTGCACAAGCA
GCACGATCGCCAAGAAATTCTTGCCTGCGAATTGCCGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(40-146)

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

54.286

95.89

0.521
  pilA2 Legionella pneumophila strain ERS1305867

52.857

95.89

0.507
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.234

100

0.479
  comP Acinetobacter baylyi ADP1

46.259

100

0.466
  pilE Neisseria gonorrhoeae strain FA1090

42.236

100

0.466
  pilE Neisseria gonorrhoeae MS11

42.236

100

0.466
  pilA Ralstonia pseudosolanacearum GMI1000

37.714

100

0.452
  pilA/pilA1 Eikenella corrodens VA1

38.854

100

0.418
  pilA/pilAI Pseudomonas stutzeri DSM 10701

36.774

100

0.39
  pilA Pseudomonas aeruginosa PAK

36.601

100

0.384

Multiple sequence alignment