Detailed information NTG0278756

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: C4901_RS13945
• Genome accession: NZ_CP027663
• Coordinates: 2750368..2751537 (-)
• Length: 389 a.a.
• NCBI ID: WP_110137849.1
• Uniprot ID: A0A2Z3R2L8
• Organism: Acidiferrobacter sp. SPIII_3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IScluster/Tn	2748719..2750233	2750368..2751537	flank	135

Gene organization within MGE regions

Location: 2748719..2751537

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C4901_RS13935 (C4901_13875)	-	2748719..2749280 (-)	562	Protein_2740	transposase	-
C4901_RS13940 (C4901_13880)	-	2749434..2750158 (-)	725	Protein_2741	IS4 family transposase	-
C4901_RS13945 (C4901_13885)	pilU	2750368..2751537 (-)	1170	WP_110137849.1	PilT/PilU family type 4a pilus ATPase	Machinery gene

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42928.18 Da
• Isoelectric Point: 7.0064

>NTDB_id=278756 C4901_RS13945 WP_110137849.1 2750368..2751537(-) (pilU) [Acidiferrobacter sp. SPIII_3]
MTPEQATASLHGLLRFMLNKKASDLFLSVDFPPAIKLDGKMTPAGQQRLTPIHTKAFAHAIMNDKQRQEFEAEKECNFAI
SPPDIGRFRVNVFVQQGHVGLVLRTITSKIPAFDDLKLPAVLRDVCLTKRGLVILVGGTGSGKSTSLAAMIDYRNEHSHG
HIITIEDPVEYVHRHKNCLITHREVGADTHNWFAALKNTLRQAPDVILIGEIRDRETMEYAIAFAETGHLCMATLHANSA
NQALDRIVNFFPEERRNQLLMDLSLNARAIVSQRLIPRADGQGRAAAVEVLLNTPLVADLIFKGEVHSLKEAMAKSREQG
MQTFDQALFDLYENGAISYEDALRNADSVNELRLAIKLRGKGAPGAAQGSPEFRVMADPEPPSTLTEAG

Nucleotide

• Length: 1170 bp

>NTDB_id=278756 C4901_RS13945 WP_110137849.1 2750368..2751537(-) (pilU) [Acidiferrobacter sp. SPIII_3]
ATGACACCCGAACAAGCCACCGCCAGCCTGCACGGCCTATTGCGTTTTATGTTGAACAAGAAGGCCTCCGATCTGTTTTT
ATCCGTCGATTTCCCGCCCGCCATAAAGCTCGACGGCAAGATGACGCCGGCCGGCCAGCAGCGCCTCACGCCGATCCACA
CCAAGGCGTTCGCCCACGCCATCATGAACGACAAGCAGCGCCAGGAATTCGAGGCCGAAAAGGAATGCAATTTCGCCATA
AGCCCGCCCGACATCGGCCGGTTTCGTGTCAACGTATTCGTTCAGCAGGGGCATGTGGGGCTGGTGTTGCGGACGATCAC
CAGCAAGATTCCGGCGTTTGATGACCTGAAGCTTCCCGCGGTGTTGCGTGATGTGTGTCTTACAAAGCGCGGCCTCGTGA
TCCTGGTCGGCGGCACCGGTTCCGGGAAATCGACCTCGCTTGCGGCGATGATCGATTACCGCAATGAACACTCCCACGGT
CATATCATCACCATCGAAGACCCCGTGGAGTATGTGCACCGCCACAAGAACTGTCTCATTACCCATCGCGAGGTCGGGGC
TGACACGCATAACTGGTTCGCGGCGTTGAAGAACACGCTGCGCCAGGCCCCGGATGTGATTTTGATCGGCGAGATCCGGG
ATCGCGAGACGATGGAATACGCAATCGCCTTTGCCGAGACCGGGCACCTGTGCATGGCCACCCTGCACGCCAACAGCGCC
AATCAGGCCCTGGACCGGATCGTGAATTTTTTCCCCGAGGAACGGCGCAATCAGTTGTTGATGGACTTGTCGTTGAATGC
GCGCGCCATCGTCTCCCAGCGGCTCATCCCGCGCGCCGACGGACAGGGGCGCGCGGCGGCAGTCGAGGTCTTGCTCAATA
CGCCCTTGGTGGCGGATCTGATCTTCAAGGGCGAGGTCCATTCCTTGAAGGAGGCGATGGCGAAGTCACGCGAGCAGGGC
ATGCAGACCTTCGATCAGGCGTTGTTCGACCTCTACGAGAATGGCGCCATCAGCTATGAGGATGCGCTGCGCAATGCCGA
TTCCGTCAACGAGCTGCGGCTTGCCATAAAGCTGCGTGGAAAGGGCGCCCCGGGGGCTGCCCAGGGATCTCCGGAATTTC
GTGTGATGGCCGACCCCGAACCCCCCTCCACGCTGACCGAGGCCGGTTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (121-277)

3D structure

• AlphaFold DB: A0A2Z3R2L8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	62.428	88.946	0.555
pilU	Acinetobacter baylyi ADP1	57.429	89.974	0.517
pilU	Vibrio cholerae strain A1552	54.571	89.974	0.491
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.895	88.432	0.388
pilT	Pseudomonas aeruginosa PAK	43.413	85.861	0.373
pilT	Pseudomonas stutzeri DSM 10701	42.814	85.861	0.368
pilT	Acinetobacter baumannii D1279779	43.03	84.833	0.365
pilT	Acinetobacter baumannii strain A118	43.03	84.833	0.365
pilT	Acinetobacter nosocomialis M2	43.03	84.833	0.365
pilT	Acinetobacter baylyi ADP1	42.727	84.833	0.362