Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C2E19_RS25235 Genome accession   NZ_CP027218
Coordinates   5508220..5509437 (+) Length   405 a.a.
NCBI ID   WP_129395555.1    Uniprot ID   A0A481R2I1
Organism   Pseudomonas sp. DTU12.3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5503220..5514437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2E19_RS25215 (C2E19_25255) - 5504678..5504974 (+) 297 WP_129395551.1 DUF2845 domain-containing protein -
  C2E19_RS25220 (C2E19_25260) - 5505209..5505562 (-) 354 WP_129395552.1 BON domain-containing protein -
  C2E19_RS25225 (C2E19_25265) - 5505859..5506290 (-) 432 WP_129395553.1 pilin -
  C2E19_RS25230 (C2E19_25270) pilB 5506517..5508217 (+) 1701 WP_129395554.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C2E19_RS25235 (C2E19_25275) pilC 5508220..5509437 (+) 1218 WP_129395555.1 type II secretion system F family protein Machinery gene
  C2E19_RS25240 (C2E19_25280) pilD 5509439..5510311 (+) 873 WP_129395556.1 A24 family peptidase Machinery gene
  C2E19_RS25245 (C2E19_25285) coaE 5510308..5510931 (+) 624 WP_129395557.1 dephospho-CoA kinase -
  C2E19_RS25250 (C2E19_25290) yacG 5510928..5511128 (+) 201 WP_129395558.1 DNA gyrase inhibitor YacG -
  C2E19_RS25255 (C2E19_25295) - 5511135..5511350 (-) 216 WP_007909901.1 hypothetical protein -
  C2E19_RS25260 (C2E19_25300) - 5511416..5512105 (-) 690 WP_129395559.1 energy-coupling factor ABC transporter permease -
  C2E19_RS25265 (C2E19_25305) - 5512285..5512911 (+) 627 WP_064392452.1 DUF1780 domain-containing protein -
  C2E19_RS25270 (C2E19_25310) - 5512908..5513435 (+) 528 WP_129395560.1 MOSC domain-containing protein -
  C2E19_RS25275 (C2E19_25315) - 5513487..5513660 (+) 174 WP_007909875.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44060.82 Da        Isoelectric Point: 10.1772

>NTDB_id=275802 C2E19_RS25235 WP_129395555.1 5508220..5509437(+) (pilC) [Pseudomonas sp. DTU12.3]
MAVKAAKISIYAWEGTDRKGSKVTGELSGQNPALIKAQLRKQGINPGKVRKKSASLLSFGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPAMRKLVDDVKQEVAAGNSFATALRKKPQYFDELYCNLVDAGEQSGALDTLLERVATYKEKS
ERLKAKIKKAMTYPSAVLLVAAVVTGILLVKVVPQFQSVFSGFGAELPAFTLMVISLSAFMQQWWWAILGVLVVAIFGTR
HALKTSQALRDRKDTWLLKLPLVGTLLYKSAVARFARTLSTTFAAGVPLVEALDSVAGATGNVVFKRAVLRIRQDVSTGM
QLNFSMRSTGVFPNMAVQMTAIGEESGALDEMLDKVAGFYEEEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=275802 C2E19_RS25235 WP_129395555.1 5508220..5509437(+) (pilC) [Pseudomonas sp. DTU12.3]
ATGGCGGTCAAGGCAGCGAAAATCAGCATCTATGCCTGGGAAGGCACGGACCGCAAAGGCAGCAAGGTCACCGGGGAACT
GAGCGGGCAAAATCCCGCACTGATCAAGGCGCAGCTGCGCAAACAGGGGATCAACCCTGGCAAGGTACGCAAGAAGTCCG
CCTCTTTGCTGAGTTTCGGCAAACGCATCAAGGCGCAGGACATCGCCCTGTTCACCCGCCAGATGGCGACCATGATGAAG
GCCGGCGTGCCGCTGTTGCAGTCGTTCGACATCATTGGCGAAGGCTTCGAAAACCCGGCCATGCGCAAACTGGTGGACGA
CGTGAAACAGGAAGTCGCGGCCGGTAACAGCTTCGCCACGGCCCTGCGCAAGAAGCCACAATATTTCGACGAGTTGTATT
GCAACCTCGTCGATGCCGGCGAGCAGTCCGGCGCCCTCGACACGCTGCTCGAGCGCGTCGCGACCTATAAGGAGAAAAGC
GAACGCCTGAAGGCCAAGATCAAGAAAGCCATGACCTACCCGTCGGCGGTGTTATTGGTCGCGGCCGTGGTGACCGGGAT
TCTGCTGGTAAAAGTGGTGCCGCAATTCCAGTCGGTATTTTCCGGTTTCGGCGCCGAGTTGCCCGCCTTTACCCTGATGG
TCATCAGCCTGTCGGCGTTCATGCAGCAATGGTGGTGGGCGATCCTCGGCGTATTGGTGGTGGCGATTTTCGGCACCCGT
CATGCGCTGAAAACATCTCAGGCCTTGCGCGACCGCAAAGACACCTGGCTGCTGAAACTGCCCTTGGTCGGCACGCTGCT
GTACAAGTCTGCCGTCGCCCGCTTCGCCCGCACCCTGTCGACGACGTTCGCCGCCGGTGTGCCGCTGGTCGAGGCGCTCG
ACTCGGTTGCGGGTGCCACCGGCAACGTGGTGTTCAAACGCGCGGTGCTGCGGATTCGTCAGGACGTCTCGACCGGTATG
CAGCTGAATTTTTCCATGCGCTCCACCGGGGTCTTTCCCAATATGGCCGTGCAGATGACCGCGATCGGCGAAGAGTCCGG
TGCGCTGGACGAGATGCTCGACAAGGTCGCCGGGTTTTACGAAGAGGAAGTGGATAACATGGTCGACAACCTCACCAGCC
TCATGGAGCCATTCATCATGGTGGTGCTGGGGGTGATCGTCGGCGGGTTGGTCGTGGCCATGTACTTGCCGATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A481R2I1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.568

100

0.746
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

59.069

100

0.595
  pilC Legionella pneumophila strain ERS1305867

53.788

97.778

0.526
  pilC Vibrio cholerae strain A1552

44.192

97.778

0.432
  pilG Neisseria gonorrhoeae MS11

42.928

99.506

0.427
  pilG Neisseria meningitidis 44/76-A

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.162

100

0.42
  pilC Thermus thermophilus HB27

38.653

99.012

0.383

Multiple sequence alignment