Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C2E19_RS25230 Genome accession   NZ_CP027218
Coordinates   5506517..5508217 (+) Length   566 a.a.
NCBI ID   WP_129395554.1    Uniprot ID   A0A481R1H9
Organism   Pseudomonas sp. DTU12.3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5501517..5513217
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2E19_RS25210 (C2E19_25250) - 5501727..5504678 (+) 2952 WP_129395550.1 DUF748 domain-containing protein -
  C2E19_RS25215 (C2E19_25255) - 5504678..5504974 (+) 297 WP_129395551.1 DUF2845 domain-containing protein -
  C2E19_RS25220 (C2E19_25260) - 5505209..5505562 (-) 354 WP_129395552.1 BON domain-containing protein -
  C2E19_RS25225 (C2E19_25265) - 5505859..5506290 (-) 432 WP_129395553.1 pilin -
  C2E19_RS25230 (C2E19_25270) pilB 5506517..5508217 (+) 1701 WP_129395554.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C2E19_RS25235 (C2E19_25275) pilC 5508220..5509437 (+) 1218 WP_129395555.1 type II secretion system F family protein Machinery gene
  C2E19_RS25240 (C2E19_25280) pilD 5509439..5510311 (+) 873 WP_129395556.1 A24 family peptidase Machinery gene
  C2E19_RS25245 (C2E19_25285) coaE 5510308..5510931 (+) 624 WP_129395557.1 dephospho-CoA kinase -
  C2E19_RS25250 (C2E19_25290) yacG 5510928..5511128 (+) 201 WP_129395558.1 DNA gyrase inhibitor YacG -
  C2E19_RS25255 (C2E19_25295) - 5511135..5511350 (-) 216 WP_007909901.1 hypothetical protein -
  C2E19_RS25260 (C2E19_25300) - 5511416..5512105 (-) 690 WP_129395559.1 energy-coupling factor ABC transporter permease -
  C2E19_RS25265 (C2E19_25305) - 5512285..5512911 (+) 627 WP_064392452.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62594.57 Da        Isoelectric Point: 6.0469

>NTDB_id=275801 C2E19_RS25230 WP_129395554.1 5506517..5508217(+) (pilB) [Pseudomonas sp. DTU12.3]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTEAIEKFFDTHASGLEDMADVDLDG
LDVEAIDDSKQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGIDALGYEPAQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLLKEGFPE
ERIGHFTIYEPVGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDSQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=275801 C2E19_RS25230 WP_129395554.1 5506517..5508217(+) (pilB) [Pseudomonas sp. DTU12.3]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTGCAGGCCGAACTGCTTACGGAAAAAAGCGCCCAGCAGGC
CTGGCAGCAGGCCCAGCGCAATCGGCTGTCGCTGGTCAGTTATCTGGTGCAGAACAAACTGGTGAAGAGCTCGCAGGTGG
CGGAAATCGCCTCGGAGCATTTCGGCATGGCGTTCCTTGATCTCAATTGCCTGGACAAGGAAACCCAGCCCAAGGGACTG
GTCAGCGAAAAACTCGTGCGCCAGCATCACGCCCTGCCCTTGTGGCGGCGTGGCAACAAGCTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAGGCGATCAACGACATCCAGTTCAGCACTGGGCTGAGTACCGAGGCCATTCTGGTCGAGGACGACA
AACTCACCGAGGCCATCGAAAAATTTTTCGACACCCACGCCAGTGGCCTCGAAGACATGGCCGATGTCGATCTCGACGGC
CTTGATGTCGAAGCCATCGACGACAGCAAGCAGGACGCCATTGGCGGACTCGACGCCGACGATGCGCCGGTGGTGCGTTT
CGTCCACAAGATGCTGCTCGACGCGATCAAGAGCGGCTCGTCCGACCTGCACTTCGAACCTTACGAGAAGAACTATCGAG
TGCGCGTGCGCACCGACGGCATCCTGCGCGAAGTGGCCAAGCCACCGATCCAGTTGGCCGGGCGCATCGCCGCGCGCCTG
AAAGTCATGGCCAGCCTCGATATCTCGGAACGGCGCAAACCGCAGGACGGGCGGATCAAGATGCGTCTGTCGAAAAGCAA
GTCGATCGATTTCCGGGTCAACACGCTGCCGACCCTGTGGGGCGAAAAAGTCGTGATCCGGATTCTCGACCCGTCCAGCG
CGCAGATCGGCATCGATGCCCTCGGCTATGAACCGGCGCAGAAAGACCTGTACATGGCTGCACTCAAGCAACCGCAAGGG
ATGATCCTGGTCACCGGCCCCACCGGTTCGGGCAAGACCGTGTCGTTGTACACCGGCCTGAATATCCTCAACACCGTCGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAGCAGG
GGCTGGACTTTGCCCAGGCCCTGCGCTCGTTTCTGCGGCAGGATCCGGACGTGATCATGGTCGGCGAGATTCGTGATCTG
GAAACCGCCGAGATCGCCATCAAGGCGGCACAGACCGGTCACCTGGTACTGTCGACCCTGCACACCAACAGCGCCGCGGA
AACCCTGACCCGTTTGCACAATATGGGCATCCCCGGTTTCAACATCGCCACGTCGGTCAGCCTGATCATCGCCCAGCGCC
TGGCGCGCAAGTTATGCAGCCACTGCAAAAAACCCATCGAGATCCCGCGCGAAACTCTGCTCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTACGAGCCGGTCGGTTGCGATCACTGCAACGGAGGATACAAGGGTCGCGTGGGGAT
TTATGAAGTGGTGAAAAACACACCGGAGCTGCAACGGCTGATCATGGCCGAGGGCAACTCGCTGGAAATCGACAGCCAGA
TGCGCCGCGACGGCTTCGACGACCTGCGCACCTCAGGGCTGCACAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A481R1H9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.688

99.647

0.585
  pilB Acinetobacter baylyi ADP1

58.584

99.823

0.585
  pilB Legionella pneumophila strain ERS1305867

56.184

100

0.562
  pilB Vibrio cholerae strain A1552

51.327

99.823

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

50.356

99.293

0.5
  pilF Neisseria gonorrhoeae MS11

49.734

99.47

0.495
  pilB Vibrio campbellii strain DS40M4

48.611

100

0.495
  pilF Thermus thermophilus HB27

37.097

98.587

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.826

93.286

0.362

Multiple sequence alignment