Detailed information NTG0027498

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: APJL_RS01495
• Genome accession: NC_010278
• Coordinates: 316566..317570 (+)
• Length: 334 a.a.
• NCBI ID: WP_005600359.1
• Uniprot ID: A3MZ06
• Organism: Actinobacillus pleuropneumoniae serovar 3 str. JL03
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 311566..322570

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
APJL_RS01475 (APJL_0290)	potA	313069..314184 (+)	1116	WP_005600350.1	spermidine/putrescine ABC transporter ATP-binding protein PotA	-
APJL_RS01480 (APJL_0291)	potB	314171..315037 (+)	867	WP_012262758.1	spermidine/putrescine ABC transporter permease PotB	-
APJL_RS01485 (APJL_0292)	potC	315037..315804 (+)	768	WP_012262759.1	spermidine/putrescine ABC transporter permease PotC	-
APJL_RS01490 (APJL_0293)	ruvA	315941..316547 (+)	607	Protein_283	Holliday junction branch migration protein RuvA	-
APJL_RS01495 (APJL_0295)	ruvB	316566..317570 (+)	1005	WP_005600359.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
APJL_RS01500 (APJL_0296)	ubiG	317627..318331 (-)	705	WP_043947927.1	bifunctional 2-polyprenyl-6-hydroxyphenol methylase/3-demethylubiquinol 3-O-methyltransferase UbiG	-
APJL_RS01505 (APJL_0297)	gyrA	318511..321201 (+)	2691	WP_012262761.1	DNA topoisomerase (ATP-hydrolyzing) subunit A	-

Sequence

Protein

• Length: 334 a.a.
• Molecular weight: 36929.33 Da
• Isoelectric Point: 4.7294

>NTDB_id=27498 APJL_RS01495 WP_005600359.1 316566..317570(+) (ruvB) [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
MIEADRIISASPKREEEVIDRAIRPKLLADYVGQPSVREQMEIFIKAAKLRDEALDHLLIFGPPGLGKTTLANIVANEMG
VNIRTTSGPVLEKAGDLAAMLTNLEPYDVLFIDEIHRLSPAIEEVLYPAMEDYQLDIMIGEGPAARSIKLDLPPFTLVGA
TTRAGSLTSPLRDRFGIVQRLEFYSVDDLTSIVKRSADCLNLNLSPDGAYEVARRSRGTPRIANRLLRRVRDYADVRNNG
VITSDIAKQALAMLDVDSEGFDFMDIKLLQAIVERFDGGPVGLDNLAAAIGEERDTIEDVLEPYLIQQGFLQRTPRGRIA
TSRTYAHLGIAKLD

Nucleotide

• Length: 1005 bp

>NTDB_id=27498 APJL_RS01495 WP_005600359.1 316566..317570(+) (ruvB) [Actinobacillus pleuropneumoniae serovar 3 str. JL03]
ATGATTGAAGCAGATAGAATAATTAGTGCCTCGCCGAAGCGTGAGGAAGAAGTCATCGATCGTGCGATTCGTCCTAAATT
ATTAGCGGATTATGTCGGTCAGCCGTCTGTACGAGAGCAGATGGAGATCTTTATTAAAGCGGCAAAATTGCGTGATGAGG
CGCTAGATCACTTATTAATTTTCGGCCCTCCGGGATTGGGTAAAACCACCTTAGCTAATATTGTTGCCAACGAAATGGGC
GTGAATATTCGTACCACTTCCGGACCTGTGTTAGAGAAAGCGGGCGATTTGGCGGCAATGCTGACCAATTTAGAACCTTA
TGACGTATTATTTATTGATGAGATCCACCGCTTATCGCCGGCAATTGAAGAAGTGCTTTATCCGGCGATGGAAGATTATC
AGCTGGATATCATGATCGGTGAAGGGCCGGCGGCACGTTCGATTAAGTTGGACTTGCCTCCGTTTACTCTAGTCGGTGCA
ACGACCCGTGCCGGTTCATTAACGTCTCCACTACGTGACCGCTTTGGTATTGTACAACGTTTAGAGTTTTACTCTGTAGA
TGATTTAACCTCGATCGTAAAACGCAGTGCCGATTGCTTAAACTTAAATTTATCGCCGGATGGTGCTTATGAAGTGGCTC
GCCGTTCACGAGGCACACCACGTATTGCCAATCGTTTATTACGCCGTGTGCGAGATTATGCCGATGTACGTAATAACGGC
GTGATTACCTCTGATATTGCCAAACAAGCGTTAGCAATGCTTGATGTAGATTCCGAAGGCTTTGACTTTATGGATATCAA
GTTACTGCAAGCGATTGTGGAACGTTTTGACGGAGGCCCGGTCGGCTTAGATAACTTAGCCGCAGCAATTGGTGAGGAGC
GAGATACGATTGAAGACGTACTTGAACCTTATTTGATTCAGCAGGGTTTTTTACAGCGAACGCCACGAGGGCGTATTGCT
ACTAGCCGAACTTATGCACATTTAGGCATTGCAAAATTAGATTAA

Domains

Predicted by InterProScan.

• RuvB C-terminal winged helix domain (WHD_RuvB) (259-328)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (23-181)

• RuvB AAA lid domain (AAA_lid_4) (184-257)

3D structure

• AlphaFold DB: A3MZ06

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	62.346	97.006	0.605
ruvB	Streptococcus pneumoniae TIGR4	59.259	97.006	0.575
ruvB	Streptococcus pneumoniae R6	59.259	97.006	0.575
ruvB	Streptococcus pneumoniae D39	59.259	97.006	0.575
ruvB	Synechocystis sp. PCC 6803	54.969	96.407	0.53
ruvB	Helicobacter pylori 26695	54.952	93.713	0.515