Detailed information NTG0027502

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: SSA_RS01100
• Genome accession: NC_009009
• Coordinates: 191155..191583 (+)
• Length: 142 a.a.
• NCBI ID: WP_011836363.1
• Uniprot ID: A3CKE6
• Organism: Streptococcus sanguinis SK36
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 186155..196583

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SSA_RS01065 (SSA_0182)	-	186361..187467 (+)	1107	WP_033179098.1	glycosyl hydrolase family 8	-
SSA_RS01070 (SSA_0183)	-	187786..188166 (+)	381	WP_011836357.1	DUF1033 family protein	-
SSA_RS01075 (SSA_0184)	comYA	188296..189237 (+)	942	WP_011836358.1	competence type IV pilus ATPase ComGA	Machinery gene
SSA_RS01080 (SSA_0185)	comYB	189167..190201 (+)	1035	WP_260469263.1	competence type IV pilus assembly protein ComGB	Machinery gene
SSA_RS01085 (SSA_0186)	comYC	190201..190518 (+)	318	WP_011836360.1	competence type IV pilus major pilin ComGC	Machinery gene
SSA_RS01090 (SSA_0187)	comYD	190478..190912 (+)	435	WP_033179099.1	competence type IV pilus minor pilin ComGD	Machinery gene
SSA_RS01095 (SSA_0188)	comGE/cglE	190878..191171 (+)	294	WP_033179100.1	competence type IV pilus minor pilin ComGE	Machinery gene
SSA_RS01100 (SSA_0189)	comGF/cglF	191155..191583 (+)	429	WP_011836363.1	competence type IV pilus minor pilin ComGF	Machinery gene
SSA_RS01105 (SSA_0190)	comGG	191573..191965 (+)	393	WP_033179101.1	competence type IV pilus minor pilin ComGG	-
SSA_RS01110 (SSA_0191)	comYH	192047..193012 (+)	966	WP_002910847.1	class I SAM-dependent methyltransferase	Machinery gene
SSA_RS01115 (SSA_0192)	-	193049..194242 (+)	1194	WP_002911070.1	acetate kinase	-
SSA_RS01120 (SSA_0193)	-	194386..195135 (+)	750	WP_033179102.1	CPBP family intramembrane glutamic endopeptidase	-
SSA_RS01125 (SSA_0195)	-	195253..195891 (+)	639	WP_011836366.1	type II CAAX prenyl endopeptidase Rce1 family protein	-

Sequence

Protein

• Length: 142 a.a.
• Molecular weight: 16407.70 Da
• Isoelectric Point: 6.2485

>NTDB_id=27502 SSA_RS01100 WP_011836363.1 191155..191583(+) (comGF/cglF) [Streptococcus sanguinis SK36]
MSKNLKVKAFTLLEALVALLVLSGGVLVFQAMTQLLSSELHQQENNQQQEWLLFADQLETELSRSQFDKVEDNKIYIRQD
GRDLALGKSKGDDFRKTDKSGRGYQPMIYGLEAADVRQDGKLIHLHFRFEKGLEREFVYRVE

Nucleotide

• Length: 429 bp

>NTDB_id=27502 SSA_RS01100 WP_011836363.1 191155..191583(+) (comGF/cglF) [Streptococcus sanguinis SK36]
GTGTCCAAAAACCTTAAAGTCAAGGCCTTTACTCTCTTGGAGGCTTTAGTAGCCTTGCTGGTTCTCAGTGGCGGTGTGTT
AGTCTTTCAGGCCATGACCCAGCTCTTATCTTCAGAACTGCATCAGCAGGAGAATAATCAGCAGCAAGAGTGGCTTTTGT
TTGCCGACCAACTGGAGACGGAGCTTTCGCGAAGTCAATTTGACAAGGTAGAAGACAATAAAATCTACATCAGACAAGAT
GGCAGGGACCTGGCCTTGGGTAAATCTAAGGGCGATGATTTCCGTAAAACAGATAAGAGTGGTCGCGGCTACCAGCCGAT
GATTTATGGTTTGGAAGCAGCTGATGTGCGTCAGGACGGTAAGCTTATCCATCTTCATTTTCGCTTTGAAAAAGGCTTAG
AGAGGGAGTTCGTCTATCGTGTGGAATAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (37-134)

3D structure

• AlphaFold DB: A3CKE6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	64.539	99.296	0.641
comGF/cglF	Streptococcus pneumoniae D39	64.539	99.296	0.641
comGF/cglF	Streptococcus pneumoniae R6	64.539	99.296	0.641
comGF/cglF	Streptococcus pneumoniae TIGR4	64.539	99.296	0.641
comGF/cglF	Streptococcus mitis SK321	63.38	100	0.634
comGF/cglF	Streptococcus mitis NCTC 12261	61.972	100	0.62
comYF	Streptococcus mutans UA159	50	94.366	0.472
comYF	Streptococcus mutans UA140	49.254	94.366	0.465
comGF	Lactococcus lactis subsp. cremoris KW2	42.647	95.775	0.408