Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MPE_RS02550 Genome accession   NC_008825
Coordinates   546037..547263 (-) Length   408 a.a.
NCBI ID   WP_011828108.1    Uniprot ID   A2SD31
Organism   Methylibium petroleiphilum PM1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541037..552263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPE_RS02515 (Mpe_A0501) slmA 541439..542101 (+) 663 WP_011828101.1 nucleoid occlusion factor SlmA -
  MPE_RS02520 (Mpe_A0502) - 542155..543066 (+) 912 WP_011828102.1 ATP-binding protein -
  MPE_RS02525 (Mpe_A0503) - 543075..543488 (+) 414 WP_011828103.1 NUDIX domain-containing protein -
  MPE_RS02530 (Mpe_A0504) - 543505..543699 (-) 195 WP_011828104.1 DNA gyrase inhibitor YacG -
  MPE_RS02535 (Mpe_A0505) zapD 543719..544471 (-) 753 WP_011828105.1 cell division protein ZapD -
  MPE_RS02540 (Mpe_A0506) coaE 544541..545164 (-) 624 WP_011828106.1 dephospho-CoA kinase -
  MPE_RS02545 (Mpe_A0507) pilD 545171..546022 (-) 852 WP_011828107.1 A24 family peptidase Machinery gene
  MPE_RS02550 (Mpe_A0508) pilC 546037..547263 (-) 1227 WP_011828108.1 type II secretion system F family protein Machinery gene
  MPE_RS02555 (Mpe_A0509) pilB 547297..549015 (-) 1719 WP_011828109.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MPE_RS02565 (Mpe_A0510) ispB 549277..550269 (-) 993 WP_011828110.1 octaprenyl diphosphate synthase -
  MPE_RS02570 (Mpe_A0511) rplU 550475..550786 (+) 312 WP_011828111.1 50S ribosomal protein L21 -
  MPE_RS02575 (Mpe_A0512) rpmA 550813..551070 (+) 258 WP_011828112.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44497.34 Da        Isoelectric Point: 9.5387

>NTDB_id=27417 MPE_RS02550 WP_011828108.1 546037..547263(-) (pilC) [Methylibium petroleiphilum PM1]
MATAATASARDIKEFVFEWEGKDKNGKVVRGEMRAGGESVVSASLRRQGILVNKVKKRRMSGGKSIKPKDIAIFTRQIAT
MMRAGVPLLQSFDIVARGSTNPRVTKLLTDIRSDIETGTSLSSAFRKHPLYFDALYCNLVEAGEAAGILETLLERLATYQ
EKTLEIKRKIKSALIYPVAVLVVAFVVVAVIMIFVIPAFKEVFTSFGADLPAPTLFVMSMSEIFVSYWWLIFGVIIGGTY
FFFESWKRSEKMQKTMDRLLLKLPVFGDLIFKSAVARWTRTLATMFAAGVPLVEALDSVGGASGNAVFAEATEQIQKDVS
TGTALTTSMQATGIFPVMVLQMAAIGEESGALDHMLSKAAEFYEAEVDEAVKGLSSLLEPIIIVVLGTIIGGIVVSMYLP
IFKLGQVV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=27417 MPE_RS02550 WP_011828108.1 546037..547263(-) (pilC) [Methylibium petroleiphilum PM1]
ATGGCCACAGCAGCAACGGCTTCGGCGCGAGACATCAAGGAATTCGTCTTCGAGTGGGAAGGCAAGGACAAGAACGGCAA
GGTCGTGCGCGGCGAGATGCGAGCCGGCGGCGAGTCGGTCGTCAGCGCGTCCCTGCGCCGCCAGGGCATCCTCGTCAACA
AGGTGAAGAAGCGCCGCATGAGCGGCGGCAAGTCCATCAAGCCGAAGGATATTGCGATCTTCACGCGCCAGATCGCGACG
ATGATGCGCGCCGGCGTTCCCTTGCTGCAGTCCTTCGACATCGTCGCGCGGGGCAGCACCAACCCTCGCGTGACCAAGCT
GCTGACCGACATCCGCTCCGACATCGAGACCGGCACCAGCCTGTCGTCGGCCTTCCGCAAGCACCCGCTGTACTTCGACG
CGCTCTACTGCAACCTCGTCGAGGCCGGTGAAGCCGCAGGTATCCTGGAAACACTGCTCGAGCGGCTGGCGACCTACCAG
GAGAAGACGCTCGAGATCAAGCGCAAGATCAAGTCGGCGCTGATCTACCCGGTGGCCGTGCTGGTGGTGGCCTTCGTGGT
CGTCGCGGTCATCATGATCTTCGTGATCCCGGCCTTCAAGGAAGTCTTCACGTCCTTCGGAGCCGACCTCCCGGCCCCGA
CGCTGTTCGTGATGTCCATGTCCGAGATCTTCGTCAGCTACTGGTGGCTGATCTTCGGCGTCATCATCGGCGGCACCTAC
TTCTTCTTCGAGTCGTGGAAGCGATCCGAGAAGATGCAGAAGACCATGGACAGGCTGCTGTTGAAGCTGCCGGTGTTCGG
TGATCTGATCTTCAAGTCGGCCGTCGCTCGCTGGACGCGCACGCTCGCCACGATGTTCGCGGCCGGCGTACCGCTGGTCG
AGGCGCTCGACTCGGTCGGTGGCGCCTCTGGCAATGCCGTGTTCGCCGAAGCGACGGAGCAGATCCAGAAGGACGTCTCC
ACCGGCACGGCACTGACCACCTCGATGCAGGCGACCGGCATCTTCCCCGTGATGGTGCTGCAGATGGCTGCCATCGGTGA
GGAATCGGGCGCCCTCGATCACATGCTGTCCAAGGCTGCCGAGTTCTACGAGGCCGAGGTCGATGAGGCCGTCAAGGGCC
TGTCGAGCCTGCTCGAGCCGATCATCATCGTCGTGCTGGGCACCATCATCGGCGGCATCGTGGTCTCGATGTACCTGCCG
ATCTTCAAGCTCGGCCAGGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(278-400)

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2SD31

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

54.478

98.529

0.537
  pilG Neisseria meningitidis 44/76-A

51.5

98.039

0.505
  pilG Neisseria gonorrhoeae MS11

51.5

98.039

0.505
  pilC Legionella pneumophila strain ERS1305867

51.768

97.059

0.502
  pilC Acinetobacter baylyi ADP1

48.744

97.549

0.475
  pilC Acinetobacter baumannii D1279779

48.111

97.304

0.468
  pilC Vibrio cholerae strain A1552

41.667

97.059

0.404
  pilC Vibrio campbellii strain DS40M4

40.399

98.284

0.397
  pilC Thermus thermophilus HB27

38.958

98.775

0.385

Multiple sequence alignment