Detailed information NTG0027416

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: MPE_RS02545
• Genome accession: NC_008825
• Coordinates: 545171..546022 (-)
• Length: 283 a.a.
• NCBI ID: WP_011828107.1
• Uniprot ID: W7WKR6
• Organism: Methylibium petroleiphilum PM1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 540171..551022

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MPE_RS02510 (Mpe_A0500)	-	540730..541461 (+)	732	WP_011828100.1	pyrimidine 5'-nucleotidase	-
MPE_RS02515 (Mpe_A0501)	slmA	541439..542101 (+)	663	WP_011828101.1	nucleoid occlusion factor SlmA	-
MPE_RS02520 (Mpe_A0502)	-	542155..543066 (+)	912	WP_011828102.1	ATP-binding protein	-
MPE_RS02525 (Mpe_A0503)	-	543075..543488 (+)	414	WP_011828103.1	NUDIX domain-containing protein	-
MPE_RS02530 (Mpe_A0504)	-	543505..543699 (-)	195	WP_011828104.1	DNA gyrase inhibitor YacG	-
MPE_RS02535 (Mpe_A0505)	zapD	543719..544471 (-)	753	WP_011828105.1	cell division protein ZapD	-
MPE_RS02540 (Mpe_A0506)	coaE	544541..545164 (-)	624	WP_011828106.1	dephospho-CoA kinase	-
MPE_RS02545 (Mpe_A0507)	pilD	545171..546022 (-)	852	WP_011828107.1	A24 family peptidase	Machinery gene
MPE_RS02550 (Mpe_A0508)	pilC	546037..547263 (-)	1227	WP_011828108.1	type II secretion system F family protein	Machinery gene
MPE_RS02555 (Mpe_A0509)	pilB	547297..549015 (-)	1719	WP_011828109.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MPE_RS02565 (Mpe_A0510)	ispB	549277..550269 (-)	993	WP_011828110.1	octaprenyl diphosphate synthase	-
MPE_RS02570 (Mpe_A0511)	rplU	550475..550786 (+)	312	WP_011828111.1	50S ribosomal protein L21	-

Sequence

Protein

• Length: 283 a.a.
• Molecular weight: 30444.23 Da
• Isoelectric Point: 8.4919

>NTDB_id=27416 MPE_RS02545 WP_011828107.1 545171..546022(-) (pilD) [Methylibium petroleiphilum PM1]
MSSDLQGVLLSPWALGLLGLCIGSFLNVVIHRLPQMMERAWRLESAELLGVTAPESPALTLAKPASRCPQCGHRIRWFEN
VPVLSWLLLRGRCSACKTPISPRYPLFEIATGLLFASLAWRFGAQPTVLAWCAFVAVLLALSGIDWDTTLLPDSLTLPLL
WAGLVAAALGWTLPLSTSVWGAVFGYLSLWSVYWLFKLTTGKEGMGAGDFKLLAALGAWLGWQMLLPIVLGASLIGAIVG
IAMKLAGALHEGRYVPFGPFLAGAGLVVLYAGAERVMGWLGWA

Nucleotide

• Length: 852 bp

>NTDB_id=27416 MPE_RS02545 WP_011828107.1 545171..546022(-) (pilD) [Methylibium petroleiphilum PM1]
ATGTCGTCCGACCTGCAGGGCGTGCTGCTGTCGCCCTGGGCTCTGGGGCTGCTCGGGCTGTGCATCGGCAGCTTTCTCAA
CGTCGTCATCCACCGCCTGCCACAGATGATGGAGCGGGCCTGGCGACTCGAAAGCGCCGAGCTGCTCGGGGTCACGGCCC
CAGAATCTCCCGCGCTCACGCTGGCGAAACCCGCCTCGCGCTGCCCGCAATGCGGTCATCGCATCCGCTGGTTCGAGAAT
GTCCCGGTGCTGAGCTGGCTGCTGTTGCGCGGACGCTGCTCAGCCTGCAAGACGCCGATCTCTCCGCGCTATCCTCTCTT
CGAGATCGCGACAGGGCTGTTGTTCGCCTCGCTGGCCTGGCGTTTCGGCGCGCAACCGACGGTGCTGGCCTGGTGCGCCT
TCGTCGCCGTCCTGCTGGCACTGTCCGGCATCGACTGGGACACGACGCTCCTGCCCGACAGCCTCACCTTGCCACTGCTC
TGGGCCGGGCTGGTGGCTGCCGCGCTCGGCTGGACGCTGCCGCTCTCGACGTCCGTCTGGGGCGCGGTGTTCGGTTATTT
GTCGCTGTGGTCGGTGTACTGGCTGTTCAAGCTCACGACTGGCAAGGAAGGCATGGGCGCGGGCGACTTCAAGCTGCTGG
CCGCCCTCGGTGCCTGGCTCGGATGGCAGATGCTGCTTCCCATCGTGCTCGGAGCTTCGTTGATCGGTGCCATCGTCGGC
ATCGCCATGAAGCTGGCTGGCGCTCTGCACGAAGGTCGCTACGTGCCCTTCGGCCCCTTCCTGGCCGGTGCCGGACTGGT
CGTCCTGTACGCAGGCGCCGAGCGCGTGATGGGCTGGCTCGGCTGGGCCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (133-241)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-122)

3D structure

• AlphaFold DB: W7WKR6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	54.307	94.346	0.512
pilD	Vibrio campbellii strain DS40M4	52.434	94.346	0.495
pilD	Neisseria gonorrhoeae MS11	47.535	100	0.477
pilD	Acinetobacter baumannii D1279779	48.519	95.406	0.463
pilD	Acinetobacter nosocomialis M2	48.148	95.406	0.459