Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MPE_RS02555 Genome accession   NC_008825
Coordinates   547297..549015 (-) Length   572 a.a.
NCBI ID   WP_011828109.1    Uniprot ID   A2SD32
Organism   Methylibium petroleiphilum PM1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 542297..554015
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MPE_RS02525 (Mpe_A0503) - 543075..543488 (+) 414 WP_011828103.1 NUDIX domain-containing protein -
  MPE_RS02530 (Mpe_A0504) - 543505..543699 (-) 195 WP_011828104.1 DNA gyrase inhibitor YacG -
  MPE_RS02535 (Mpe_A0505) zapD 543719..544471 (-) 753 WP_011828105.1 cell division protein ZapD -
  MPE_RS02540 (Mpe_A0506) coaE 544541..545164 (-) 624 WP_011828106.1 dephospho-CoA kinase -
  MPE_RS02545 (Mpe_A0507) pilD 545171..546022 (-) 852 WP_011828107.1 A24 family peptidase Machinery gene
  MPE_RS02550 (Mpe_A0508) pilC 546037..547263 (-) 1227 WP_011828108.1 type II secretion system F family protein Machinery gene
  MPE_RS02555 (Mpe_A0509) pilB 547297..549015 (-) 1719 WP_011828109.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MPE_RS02565 (Mpe_A0510) ispB 549277..550269 (-) 993 WP_011828110.1 octaprenyl diphosphate synthase -
  MPE_RS02570 (Mpe_A0511) rplU 550475..550786 (+) 312 WP_011828111.1 50S ribosomal protein L21 -
  MPE_RS02575 (Mpe_A0512) rpmA 550813..551070 (+) 258 WP_011828112.1 50S ribosomal protein L27 -
  MPE_RS02580 (Mpe_A0513) cgtA 551174..552286 (+) 1113 WP_011828113.1 Obg family GTPase CgtA -
  MPE_RS02585 (Mpe_A0514) proB 552323..553441 (+) 1119 WP_011828114.1 glutamate 5-kinase -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62301.40 Da        Isoelectric Point: 5.6284

>NTDB_id=27418 MPE_RS02555 WP_011828109.1 547297..549015(-) (pilB) [Methylibium petroleiphilum PM1]
MDTLADAPQSSLSGVARVLVHAGKLNVKTAEDLVRSAKEQKRSFISAVLSAGAVNPSDLAHTLSSVLAMPLLDLAAVDPQ
RLPRNVIDAKLATQYQVLVLGKRGNRLFIAGADPTDQEAAERIKFATQLTPEWVIVEHDKLSRLVDAATTTASESLESLV
AGDFEFDVTEEDTSASDAAEVTTDVEDAPVVRFLQKMLIDAINARASDLHFEPFEYNYRVRFRVDGELREITQPPIAIKD
KLASRIKVISRLDIAEKRVPQDGRMKLKFGSKAIDFRVSTLPTLFAEKIVIRILDPSSAKLGIEALGYEKVEKDRLLEAI
KRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGVNQVNVNDKAGLNFSVALKAFLRQDPDVIMVG
EIRDLDTADIAIKAAQTGHMVMSTLHTNDAPTTLTRLMNMGVAPFNIASSVILITAQRLARKLCENCKAPADYPREALLR
AGFAESDLDGSWKPYRAVGCSSCSNGYRGRVGIYQVMPISEEIQRIILTQGNAVDIAKQAQQEGVRDLRQSGLVKVRAGV
TTLEEVISVTNE

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=27418 MPE_RS02555 WP_011828109.1 547297..549015(-) (pilB) [Methylibium petroleiphilum PM1]
GTGGATACCCTCGCCGATGCCCCGCAGAGTTCGCTGTCCGGCGTCGCGCGCGTGCTCGTGCACGCGGGCAAGCTCAACGT
CAAGACCGCCGAAGATCTGGTCCGCAGCGCCAAGGAGCAGAAGCGCAGTTTCATCTCGGCGGTCCTGAGCGCTGGCGCCG
TCAACCCTTCGGATCTTGCCCATACGCTGTCCAGCGTGCTGGCGATGCCCTTGCTCGATCTGGCGGCGGTCGATCCGCAG
CGCCTGCCTCGCAACGTCATCGATGCCAAGCTGGCCACCCAGTATCAGGTATTGGTGCTGGGCAAGCGCGGCAACCGCCT
CTTCATCGCCGGGGCCGACCCCACCGACCAGGAGGCCGCTGAACGCATCAAGTTCGCAACGCAGCTGACGCCCGAGTGGG
TGATCGTCGAGCACGACAAGCTGTCCCGGCTCGTCGATGCAGCTACGACAACGGCCAGCGAGTCTCTCGAGTCGCTGGTC
GCCGGTGATTTCGAGTTCGACGTCACCGAGGAAGACACCAGCGCATCCGACGCCGCCGAGGTCACCACCGACGTCGAGGA
CGCACCGGTCGTTCGCTTCCTTCAGAAGATGCTGATCGATGCGATCAACGCACGAGCCTCCGATCTGCACTTCGAGCCGT
TCGAATACAACTACCGGGTCCGCTTCCGCGTCGATGGCGAACTGCGCGAGATCACGCAACCGCCGATTGCCATCAAGGAC
AAGCTCGCCTCCCGCATCAAGGTGATTTCCCGGCTCGACATTGCCGAGAAGCGCGTGCCGCAGGACGGCCGCATGAAGCT
CAAGTTCGGCAGCAAGGCGATTGATTTCCGGGTCAGCACCCTGCCCACGCTGTTCGCCGAGAAGATCGTGATCCGTATCC
TCGACCCGTCGAGTGCCAAGCTCGGCATCGAGGCGCTCGGCTACGAGAAGGTCGAGAAAGACCGCTTGCTGGAAGCCATC
AAGCGCCCCTATGGCATGGTGCTGGTCACCGGTCCCACAGGCTCCGGCAAGACGGTGTCTCTGTACACCTGCCTGAACAT
CCTGAACCAACCCGGCGTCAATATCTCCACGGTGGAGGACCCGGCCGAAATCAACCTCCCGGGCGTCAACCAGGTCAACG
TCAACGACAAGGCAGGACTGAACTTCTCGGTCGCCTTGAAGGCCTTCCTGCGGCAGGATCCGGACGTCATCATGGTCGGC
GAAATTCGCGATCTCGACACGGCGGACATCGCCATCAAGGCGGCGCAGACCGGGCACATGGTGATGAGCACGCTGCATAC
CAACGACGCGCCGACGACGCTGACCCGCCTGATGAACATGGGCGTCGCGCCGTTCAACATCGCCTCCAGCGTGATCCTGA
TCACGGCGCAGCGGCTGGCGCGCAAACTCTGCGAGAACTGCAAGGCGCCGGCCGATTACCCTCGCGAGGCGTTGCTTCGC
GCCGGCTTCGCTGAATCGGACCTGGATGGCAGTTGGAAGCCCTATCGTGCCGTCGGCTGCTCCAGTTGCAGCAATGGCTA
CCGGGGGCGTGTCGGCATCTACCAGGTCATGCCGATCAGCGAAGAGATCCAGCGCATCATCCTGACGCAGGGCAATGCGG
TCGACATCGCAAAGCAGGCACAGCAAGAGGGCGTGCGCGATTTGCGTCAGTCGGGTCTGGTCAAGGTGCGAGCGGGTGTG
ACCACCCTGGAAGAAGTCATCTCGGTCACCAACGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2SD32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.136

100

0.533
  pilF Neisseria gonorrhoeae MS11

53.819

98.427

0.53
  pilB Acinetobacter baylyi ADP1

53.108

98.427

0.523
  pilB Legionella pneumophila strain ERS1305867

50.355

98.601

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

46.441

98.252

0.456
  pilB Vibrio cholerae strain A1552

45.536

97.902

0.446
  pilB Vibrio campbellii strain DS40M4

45.357

97.902

0.444
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.658

92.133

0.365

Multiple sequence alignment