Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   RK60_RS00905 Genome accession   NZ_CP026968
Coordinates   186597..187025 (+) Length   142 a.a.
NCBI ID   WP_000934385.1    Uniprot ID   A0A2K0CLZ8
Organism   Staphylococcus aureus strain FDAARGOS_1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 178639..222352 186597..187025 within 0


Gene organization within MGE regions


Location: 178639..222352
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RK60_RS00810 (RK60_00810) - 178639..179844 (-) 1206 WP_016170637.1 tyrosine-type recombinase/integrase -
  RK60_RS00815 (RK60_00815) - 179970..180584 (+) 615 WP_000191459.1 hypothetical protein -
  RK60_RS00820 (RK60_00820) - 180581..180727 (-) 147 WP_001013104.1 hypothetical protein -
  RK60_RS00825 (RK60_00825) - 180817..181212 (-) 396 WP_001558060.1 hypothetical protein -
  RK60_RS00830 (RK60_00830) - 181241..181675 (-) 435 WP_016170817.1 hypothetical protein -
  RK60_RS00835 (RK60_00835) - 181693..182154 (-) 462 WP_000525004.1 hypothetical protein -
  RK60_RS00840 (RK60_00840) - 182167..182481 (-) 315 WP_000333630.1 helix-turn-helix domain-containing protein -
  RK60_RS00845 (RK60_00845) - 182633..182869 (+) 237 WP_001121027.1 helix-turn-helix domain-containing protein -
  RK60_RS00850 (RK60_00850) - 182883..183659 (+) 777 WP_025176283.1 Rha family transcriptional regulator -
  RK60_RS00855 (RK60_00855) - 183688..183831 (+) 144 WP_000939498.1 hypothetical protein -
  RK60_RS00860 (RK60_00860) - 183821..184030 (-) 210 WP_000642492.1 hypothetical protein -
  RK60_RS00865 (RK60_00865) - 184086..184331 (+) 246 WP_001025401.1 DUF2829 domain-containing protein -
  RK60_RS00870 (RK60_00870) - 184300..184665 (-) 366 WP_016170627.1 hypothetical protein -
  RK60_RS00875 (RK60_00875) - 184720..184935 (+) 216 WP_001097552.1 hypothetical protein -
  RK60_RS00880 (RK60_00880) - 184960..185223 (+) 264 WP_001124160.1 helix-turn-helix domain-containing protein -
  RK60_RS00885 (RK60_00885) - 185237..185398 (+) 162 WP_047211484.1 DUF1270 family protein -
  RK60_RS00890 (RK60_00890) - 185490..185750 (+) 261 WP_000291090.1 DUF1108 family protein -
  RK60_RS00895 (RK60_00895) - 185760..185981 (+) 222 WP_000815401.1 DUF2483 family protein -
  RK60_RS00900 (RK60_00900) - 185974..186597 (+) 624 WP_000139720.1 DUF1071 domain-containing protein -
  RK60_RS00905 (RK60_00905) ssbA 186597..187025 (+) 429 WP_000934385.1 single-stranded DNA-binding protein Machinery gene
  RK60_RS00910 (RK60_00910) - 187039..187713 (+) 675 WP_031888948.1 putative HNHc nuclease -
  RK60_RS00915 (RK60_00915) - 187820..188668 (-) 849 WP_000371251.1 DUF4393 domain-containing protein -
  RK60_RS00920 (RK60_00920) - 188732..189445 (+) 714 WP_000132054.1 helix-turn-helix domain-containing protein -
  RK60_RS00925 (RK60_00925) - 189455..190234 (+) 780 WP_000803056.1 ATP-binding protein -
  RK60_RS15030 - 190228..190389 (+) 162 WP_000237153.1 hypothetical protein -
  RK60_RS00930 (RK60_00930) - 190402..190623 (+) 222 WP_001123673.1 DUF3269 family protein -
  RK60_RS00935 (RK60_00935) - 190633..191037 (+) 405 Protein_182 DUF1064 domain-containing protein -
  RK60_RS00940 (RK60_00940) - 191042..191227 (+) 186 WP_001187252.1 DUF3113 family protein -
  RK60_RS00945 (RK60_00945) - 191228..191845 (+) 618 WP_000907339.1 SA1788 family PVL leukocidin-associated protein -
  RK60_RS00950 (RK60_00950) - 191845..192099 (+) 255 WP_000022736.1 DUF3310 domain-containing protein -
  RK60_RS00955 (RK60_00955) - 192099..192347 (+) 249 WP_060474161.1 SAV1978 family virulence-associated passenger protein -
  RK60_RS00960 (RK60_00960) - 192361..192567 (+) 207 WP_000693987.1 hypothetical protein -
  RK60_RS00965 (RK60_00965) - 192570..192974 (+) 405 WP_000695756.1 hypothetical protein -
  RK60_RS00970 (RK60_00970) - 192971..193165 (+) 195 WP_000983953.1 hypothetical protein -
  RK60_RS15095 (RK60_00975) - 193204..193533 (+) 330 WP_318655701.1 YopX family protein -
  RK60_RS00980 (RK60_00980) - 193530..193919 (+) 390 WP_016060009.1 hypothetical protein -
  RK60_RS00985 (RK60_00985) - 193912..194160 (+) 249 WP_001065061.1 DUF1024 family protein -
  RK60_RS00990 (RK60_00990) - 194153..194689 (+) 537 WP_000185671.1 dUTPase -
  RK60_RS00995 (RK60_00995) - 194726..194932 (+) 207 WP_000195794.1 DUF1381 domain-containing protein -
  RK60_RS01000 (RK60_01000) - 194929..195132 (+) 204 WP_000141180.1 hypothetical protein -
  RK60_RS01005 (RK60_01005) - 195125..195361 (+) 237 WP_000608275.1 hypothetical protein -
  RK60_RS01010 (RK60_01010) - 195351..195740 (+) 390 WP_171012236.1 hypothetical protein -
  RK60_RS01015 (RK60_01015) rinB 195737..195889 (+) 153 WP_000595263.1 transcriptional activator RinB -
  RK60_RS01020 (RK60_01020) - 195957..196157 (+) 201 WP_000265252.1 DUF1514 family protein -
  RK60_RS15105 - 197144..197716 (+) 573 Protein_200 helicase-related protein -
  RK60_RS01035 (RK60_01035) - 197729..198166 (+) 438 WP_000513701.1 transcriptional regulator -
  RK60_RS01040 (RK60_01040) - 198328..198642 (+) 315 WP_000196700.1 HNH endonuclease -
  RK60_RS01045 (RK60_01045) - 198768..199073 (+) 306 WP_000778930.1 P27 family phage terminase small subunit -
  RK60_RS01050 (RK60_01050) - 199063..200754 (+) 1692 WP_000153549.1 terminase large subunit -
  RK60_RS01055 (RK60_01055) - 200759..201997 (+) 1239 WP_001100670.1 phage portal protein -
  RK60_RS01060 (RK60_01060) - 201981..202754 (+) 774 WP_000061868.1 head maturation protease, ClpP-related -
  RK60_RS01065 (RK60_01065) - 202766..203929 (+) 1164 WP_001142735.1 phage major capsid protein -
  RK60_RS01070 (RK60_01070) - 203998..204276 (+) 279 WP_000050975.1 head-tail connector protein -
  RK60_RS01075 (RK60_01075) - 204288..204620 (+) 333 WP_000395501.1 hypothetical protein -
  RK60_RS01080 (RK60_01080) - 204617..205018 (+) 402 WP_000110020.1 hypothetical protein -
  RK60_RS01085 (RK60_01085) - 205019..205414 (+) 396 WP_001023802.1 DUF3168 domain-containing protein -
  RK60_RS01090 (RK60_01090) - 205449..206090 (+) 642 WP_000807540.1 major tail protein -
  RK60_RS01095 (RK60_01095) - 206182..206637 (+) 456 WP_000169127.1 Ig-like domain-containing protein -
  RK60_RS01100 (RK60_01100) gpG 206695..207045 (+) 351 WP_000589167.1 phage tail assembly chaperone G -
  RK60_RS01105 (RK60_01105) - 207087..207245 (+) 159 WP_000438833.1 hypothetical protein -
  RK60_RS01110 (RK60_01110) - 207259..213459 (+) 6201 WP_047211487.1 phage tail tape measure protein -
  RK60_RS01115 (RK60_01115) - 213459..214283 (+) 825 WP_001190533.1 phage tail family protein -
  RK60_RS01120 (RK60_01120) - 214292..215875 (+) 1584 WP_000384462.1 prophage endopeptidase tail family protein -
  RK60_RS01125 (RK60_01125) - 215875..216165 (+) 291 WP_000179860.1 hypothetical protein -
  RK60_RS01130 (RK60_01130) - 216181..218091 (+) 1911 WP_016170708.1 hypothetical protein -
  RK60_RS01135 (RK60_01135) - 218091..219557 (+) 1467 WP_047211488.1 BppU family phage baseplate upper protein -
  RK60_RS01140 (RK60_01140) - 219557..219946 (+) 390 WP_001166599.1 DUF2977 domain-containing protein -
  RK60_RS01145 (RK60_01145) - 219939..220103 (+) 165 WP_000916020.1 XkdX family protein -
  RK60_RS01150 (RK60_01150) - 220149..220448 (+) 300 WP_000466775.1 DUF2951 family protein -
  RK60_RS01155 (RK60_01155) - 220584..220886 (+) 303 WP_000339146.1 phage holin -
  RK60_RS01160 (RK60_01160) - 220898..222352 (+) 1455 WP_047211489.1 N-acetylmuramoyl-L-alanine amidase -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 15872.58 Da        Isoelectric Point: 8.4825

>NTDB_id=274034 RK60_RS00905 WP_000934385.1 186597..187025(+) (ssbA) [Staphylococcus aureus strain FDAARGOS_1]
MLNRAVLVGRLTKDPELRSAPNGVNVGTFTLAVNRTFTNAQGEREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTR
SYENKVGQRVFVTEVVADSVQFLEPKNNNQQPNNNYHQQRQTQTGNNPFDNTTAITDDDLPF

Nucleotide


Download         Length: 429 bp        

>NTDB_id=274034 RK60_RS00905 WP_000934385.1 186597..187025(+) (ssbA) [Staphylococcus aureus strain FDAARGOS_1]
ATGTTAAACAGAGCAGTATTAGTAGGACGCTTAACAAAAGACCCAGAATTAAGAAGCGCGCCAAATGGCGTAAATGTAGG
TACATTCACATTGGCAGTAAACAGAACATTCACGAATGCTCAAGGCGAGCGTGAAGCAGATTTTATAAACGTAGTAGTGT
TCAAGAAACAAGCTGAAAATGTTAAAAACTACCTTTCTAAAGGGTCGCTGGCAGGTGTAGACGGGCGACTACAAACACGT
AGCTACGAAAATAAAGTCGGGCAACGTGTATTTGTGACAGAAGTAGTAGCGGACAGTGTTCAATTCTTAGAACCGAAGAA
TAACAACCAACAACCAAACAACAATTATCATCAACAAAGACAAACTCAAACTGGTAATAATCCTTTTGATAATACCACTG
CGATTACTGATGATGACTTACCGTTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A2K0CLZ8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Bacillus subtilis subsp. subtilis str. 168

58.14

100

0.704

  ssb Latilactobacillus sakei subsp. sakei 23K

50.588

100

0.606

  ssbB Bacillus subtilis subsp. subtilis str. 168

58.491

74.648

0.437

  ssbB Streptococcus sobrinus strain NIDR 6715-7

41.958

100

0.423

  ssbA Streptococcus mutans UA159

40.845

100

0.408

  ssbB/cilA Streptococcus mitis NCTC 12261

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae TIGR4

40.141

100

0.401

  ssbB/cilA Streptococcus pneumoniae Rx1

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae D39

39.437

100

0.394

  ssbB/cilA Streptococcus pneumoniae R6

39.437

100

0.394

  ssbB/cilA Streptococcus mitis SK321

39.437

100

0.394


Multiple sequence alignment