Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PNAP_RS03805 Genome accession   NC_008781
Coordinates   810814..812031 (+) Length   405 a.a.
NCBI ID   WP_011800181.1    Uniprot ID   -
Organism   Polaromonas naphthalenivorans CJ2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 805814..817031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNAP_RS03775 (Pnap_0761) - 806206..806922 (-) 717 WP_011800175.1 type II secretion system protein J -
  PNAP_RS03780 (Pnap_0762) gspI 806925..807302 (-) 378 WP_011800176.1 type II secretion system minor pseudopilin GspI -
  PNAP_RS03785 (Pnap_0763) - 807302..807808 (-) 507 WP_408633749.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PNAP_RS03790 (Pnap_0764) gspG 807843..808292 (-) 450 WP_041376506.1 type II secretion system major pseudopilin GspG -
  PNAP_RS03795 (Pnap_0765) - 808451..808765 (+) 315 WP_232290748.1 hypothetical protein -
  PNAP_RS03800 (Pnap_0766) pilB 808992..810728 (+) 1737 WP_011800180.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PNAP_RS03805 (Pnap_0767) pilC 810814..812031 (+) 1218 WP_011800181.1 type II secretion system F family protein Machinery gene
  PNAP_RS03810 (Pnap_0768) pilD 812031..812882 (+) 852 WP_011800182.1 A24 family peptidase Machinery gene
  PNAP_RS03815 (Pnap_0769) coaE 812887..813498 (+) 612 WP_011800183.1 dephospho-CoA kinase -
  PNAP_RS03820 (Pnap_0770) zapD 813538..814293 (+) 756 WP_011800184.1 cell division protein ZapD -
  PNAP_RS03825 (Pnap_0771) - 814352..814558 (+) 207 WP_011800185.1 DNA gyrase inhibitor YacG -
  PNAP_RS03830 (Pnap_0772) - 814636..814896 (+) 261 WP_011800186.1 hypothetical protein -
  PNAP_RS03835 (Pnap_0773) grxD 814938..815267 (-) 330 WP_011800187.1 Grx4 family monothiol glutaredoxin -
  PNAP_RS03840 (Pnap_0774) prmC 815423..816274 (-) 852 WP_011800188.1 peptide chain release factor N(5)-glutamine methyltransferase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43957.76 Da        Isoelectric Point: 9.7360

>NTDB_id=27273 PNAP_RS03805 WP_011800181.1 810814..812031(+) (pilC) [Polaromonas naphthalenivorans CJ2]
MATAASKGIKEFVFEWEGKDRGGKQVRGEIRASGENQVKVSLRRQGVLASKIKKRRMRSGKAIKPRDIAIFTRQLATMMK
AGVPLLQSFDIVGRGNPNGSVTKLLNDVRTDVETGTSLSGAFRKYPMYFNALYCNLVEAGEAAGILESLLDRLAIYMEKT
EAIKSKIKSALMYPASVVVVAFVVVAVIMIFVIPAFKQVFSSFGAELPAPTLIVMAISEFFVAYWWLIFGGVGGGIYFFL
QAWKRSEKMQKVMDRLLLKMPVFGSLIEKSVIARWTRTLSTMFAAGVPLVEALDSVGGASGNSLYADATEKIQQEVSTGT
SLTAAMGNANLFPTMVLQMCAIGEESGSVDHMLGKAADFYEAEVDEMVAGLSSLMEPIIIVFLGTLIGGIVVSMYLPIFK
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=27273 PNAP_RS03805 WP_011800181.1 810814..812031(+) (pilC) [Polaromonas naphthalenivorans CJ2]
ATGGCAACCGCAGCATCAAAAGGCATCAAGGAATTTGTTTTCGAATGGGAAGGCAAGGACCGGGGAGGCAAACAGGTTCG
CGGAGAGATTCGCGCTTCTGGTGAAAACCAGGTCAAGGTGTCGCTGCGCCGGCAGGGCGTGCTGGCGAGCAAGATCAAGA
AACGCCGCATGCGCTCGGGCAAGGCCATCAAGCCGCGCGACATCGCCATCTTTACCCGCCAGCTTGCCACCATGATGAAG
GCCGGCGTGCCGCTGCTGCAGTCCTTTGACATTGTGGGGCGAGGCAATCCCAACGGCAGCGTGACCAAACTGCTGAACGA
TGTACGCACGGATGTGGAAACAGGAACGTCGCTCAGCGGTGCTTTCCGCAAATACCCGATGTACTTCAATGCGCTGTACT
GCAACCTGGTCGAAGCGGGCGAAGCAGCCGGTATCCTGGAGTCGCTGCTGGACCGGCTGGCCATCTATATGGAGAAAACA
GAGGCCATCAAGTCAAAGATCAAATCGGCCCTGATGTACCCAGCTTCTGTCGTGGTGGTGGCTTTTGTCGTGGTGGCCGT
GATCATGATTTTCGTGATTCCAGCATTCAAGCAGGTGTTTTCTTCCTTTGGTGCCGAGTTGCCAGCACCCACGCTCATCG
TGATGGCCATCAGCGAGTTCTTTGTGGCTTACTGGTGGCTGATTTTCGGTGGTGTGGGTGGCGGTATTTATTTTTTCCTG
CAGGCCTGGAAGCGCAGTGAAAAAATGCAGAAAGTCATGGACCGGCTGCTGCTGAAAATGCCTGTGTTCGGCAGCCTGAT
AGAAAAGTCCGTGATCGCCCGCTGGACCCGTACCCTGTCCACCATGTTTGCGGCCGGCGTGCCGCTGGTCGAAGCCCTCG
ACTCGGTGGGCGGCGCTTCGGGGAACTCCCTGTATGCCGATGCCACCGAGAAAATCCAGCAGGAAGTCTCGACCGGCACC
AGCCTGACAGCGGCCATGGGCAATGCCAACCTGTTTCCCACGATGGTGCTGCAGATGTGCGCCATTGGCGAGGAGTCGGG
CTCGGTAGACCACATGCTGGGCAAGGCGGCCGATTTTTATGAAGCCGAAGTCGATGAAATGGTGGCCGGCCTGTCCAGCC
TGATGGAGCCCATCATCATTGTATTTCTGGGCACCCTGATTGGCGGCATCGTGGTTTCCATGTACCTGCCGATTTTCAAG
CTGGGTGCGGTCGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.96

99.753

0.538
  pilG Neisseria gonorrhoeae MS11

52.5

98.765

0.519
  pilG Neisseria meningitidis 44/76-A

52.25

98.765

0.516
  pilC Legionella pneumophila strain ERS1305867

50.754

98.272

0.499
  pilC Acinetobacter baylyi ADP1

50.251

98.272

0.494
  pilC Acinetobacter baumannii D1279779

49.37

98.025

0.484
  pilC Vibrio cholerae strain A1552

40.657

97.778

0.398
  pilC Thermus thermophilus HB27

37.799

100

0.39
  pilC Vibrio campbellii strain DS40M4

38.462

99.506

0.383

Multiple sequence alignment