Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PNAP_RS03800 Genome accession   NC_008781
Coordinates   808992..810728 (+) Length   578 a.a.
NCBI ID   WP_011800180.1    Uniprot ID   A1VKA7
Organism   Polaromonas naphthalenivorans CJ2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 803992..815728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNAP_RS03770 (Pnap_0760) gspK 805178..806209 (-) 1032 WP_011800174.1 type II secretion system minor pseudopilin GspK -
  PNAP_RS03775 (Pnap_0761) - 806206..806922 (-) 717 WP_011800175.1 type II secretion system protein J -
  PNAP_RS03780 (Pnap_0762) gspI 806925..807302 (-) 378 WP_011800176.1 type II secretion system minor pseudopilin GspI -
  PNAP_RS03785 (Pnap_0763) - 807302..807808 (-) 507 WP_408633749.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PNAP_RS03790 (Pnap_0764) gspG 807843..808292 (-) 450 WP_041376506.1 type II secretion system major pseudopilin GspG -
  PNAP_RS03795 (Pnap_0765) - 808451..808765 (+) 315 WP_232290748.1 hypothetical protein -
  PNAP_RS03800 (Pnap_0766) pilB 808992..810728 (+) 1737 WP_011800180.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PNAP_RS03805 (Pnap_0767) pilC 810814..812031 (+) 1218 WP_011800181.1 type II secretion system F family protein Machinery gene
  PNAP_RS03810 (Pnap_0768) pilD 812031..812882 (+) 852 WP_011800182.1 A24 family peptidase Machinery gene
  PNAP_RS03815 (Pnap_0769) coaE 812887..813498 (+) 612 WP_011800183.1 dephospho-CoA kinase -
  PNAP_RS03820 (Pnap_0770) zapD 813538..814293 (+) 756 WP_011800184.1 cell division protein ZapD -
  PNAP_RS03825 (Pnap_0771) - 814352..814558 (+) 207 WP_011800185.1 DNA gyrase inhibitor YacG -
  PNAP_RS03830 (Pnap_0772) - 814636..814896 (+) 261 WP_011800186.1 hypothetical protein -
  PNAP_RS03835 (Pnap_0773) grxD 814938..815267 (-) 330 WP_011800187.1 Grx4 family monothiol glutaredoxin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62726.42 Da        Isoelectric Point: 5.4632

>NTDB_id=27272 PNAP_RS03800 WP_011800180.1 808992..810728(+) (pilB) [Polaromonas naphthalenivorans CJ2]
MSATDTAIKPPVSMALPGLGRALIAAGKLGQKAAEEIFLKAQASRTSFIAELTGSGAVSAFDLAHLMSTSFAAPLVDMDA
IDNQRLPKGLLDSKICLTCRIVVLGKRNNRLIVATADPSDQGAAEKIKFSTQLGVDWVIAEYDKLSRLVDVSNASAAEAI
ENIVGSDFEFDEEMADSPGVENTEASVSEVEDAPVVKFLQKMLIDAFNMRASDLHFEPFEHSYRVRFRVDGELREISSPP
VAIKEKLAARIKVISKLDISEKRVPQDGKMKLKIGPDRVIDFRVSTLPTMFGEKIVIRILDPSSAKLGIDALGYEPAEKE
RLLEAIKRPYGMVLVTGPTGSGKTVSLYTCLNILNTAGVNIATAEDPAEITLPGVNQVSMNEKAGMTFPVALKAFLRQDP
DIIMVGEIRDVETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCPHCKTPIDIP
RETLLEAGFKEDEVDGSWTPYHPVGCDKCNNGYKGRLGIYQVMPISEEIQRIVLRDGSAMEIAAQAQAEGVRSLRQSGLY
KVKLGMTSMEEILGCTNV

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=27272 PNAP_RS03800 WP_011800180.1 808992..810728(+) (pilB) [Polaromonas naphthalenivorans CJ2]
ATGTCCGCCACTGATACCGCAATAAAACCCCCAGTTTCCATGGCATTGCCTGGCTTGGGCCGGGCCTTGATCGCTGCCGG
CAAGCTGGGCCAAAAGGCGGCCGAGGAAATTTTTCTCAAGGCGCAAGCCAGTCGAACCAGTTTCATTGCCGAGCTGACAG
GCTCAGGGGCGGTGTCGGCATTCGATCTGGCGCACCTCATGTCAACGTCCTTCGCCGCGCCCCTTGTGGACATGGATGCC
ATCGACAACCAGCGTTTGCCCAAGGGCCTGCTCGACAGCAAGATATGCCTGACCTGCCGCATTGTTGTACTGGGCAAGCG
CAACAACCGACTGATCGTCGCAACCGCTGATCCATCGGATCAGGGTGCTGCGGAAAAAATCAAGTTTTCTACCCAGCTGG
GCGTGGACTGGGTCATTGCCGAATACGACAAGCTGTCCCGGCTGGTGGATGTTTCCAACGCCTCGGCTGCCGAAGCGATT
GAAAACATCGTGGGCTCGGATTTCGAATTTGATGAGGAGATGGCGGATTCGCCCGGTGTAGAAAACACTGAAGCCAGCGT
GTCGGAAGTTGAAGACGCGCCGGTTGTCAAGTTCCTGCAGAAAATGCTGATAGACGCTTTCAACATGCGTGCTTCCGATC
TGCACTTTGAACCTTTCGAGCATTCCTACCGGGTCCGGTTTCGGGTCGATGGGGAGTTGCGTGAAATTTCTTCTCCGCCG
GTCGCCATCAAGGAAAAGCTGGCAGCGCGCATCAAGGTGATTTCCAAGCTGGACATTTCCGAGAAACGGGTGCCGCAGGA
CGGCAAGATGAAGCTCAAGATTGGTCCTGACCGGGTGATCGACTTCCGGGTCAGTACCCTGCCGACCATGTTTGGCGAAA
AAATCGTGATTCGTATCCTGGATCCGAGCAGCGCCAAACTGGGGATTGATGCGCTGGGTTATGAGCCGGCTGAAAAGGAG
CGCTTGCTGGAGGCCATCAAACGGCCTTACGGCATGGTTCTGGTCACCGGGCCGACGGGTTCGGGCAAAACCGTGTCGCT
CTACACCTGCCTGAACATTTTGAATACCGCCGGCGTCAACATCGCCACCGCAGAAGATCCGGCTGAAATCACTTTGCCCG
GCGTGAACCAGGTCAGCATGAACGAGAAAGCCGGAATGACTTTTCCGGTGGCCCTGAAGGCCTTTTTGCGCCAGGATCCG
GACATCATCATGGTGGGCGAAATCCGCGATGTCGAAACCGCCGACATTGCCATCAAGGCCGCGCAGACCGGCCATCTGGT
TCTGTCCACGCTGCATACCAACGATGCGCCGACGACCCTGACACGCATGCGCAACATGGGCATTGCGCCTTTCAACATCG
CGTCGAGCGTCATTCTGATTACGGCCCAGAGGCTGGCGCGGCGCCTGTGCCCGCACTGCAAAACCCCGATAGATATTCCG
CGTGAAACATTGCTTGAGGCCGGCTTTAAAGAGGATGAAGTGGACGGCTCATGGACGCCCTATCATCCGGTGGGCTGCGA
CAAGTGCAACAACGGCTACAAGGGCCGCTTGGGCATTTACCAGGTCATGCCAATTTCCGAGGAAATTCAGCGCATTGTCC
TGCGTGATGGCAGTGCCATGGAAATTGCAGCCCAGGCCCAGGCTGAAGGTGTCAGGAGCCTGCGCCAGTCCGGTTTGTAC
AAAGTTAAACTGGGAATGACTTCAATGGAAGAAATTCTGGGCTGCACCAATGTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-150)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A1VKA7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

52.182

99.135

0.517
  pilB Acinetobacter baumannii D1279779

52.007

99.135

0.516
  pilF Neisseria gonorrhoeae MS11

51.064

97.578

0.498
  pilB Legionella pneumophila strain ERS1305867

49.02

97.059

0.476
  pilB Vibrio cholerae strain A1552

45.704

94.637

0.433
  pilB Vibrio parahaemolyticus RIMD 2210633

44.326

97.578

0.433
  pilB Vibrio campbellii strain DS40M4

44.207

97.059

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.15

92.215

0.37
  pilF Thermus thermophilus HB27

37.544

97.232

0.365

Multiple sequence alignment