Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PH4a_RS02200 Genome accession   NZ_CP026364
Coordinates   454121..455221 (+) Length   366 a.a.
NCBI ID   WP_129037426.1    Uniprot ID   -
Organism   Proteus hauseri strain 15H5D-4a     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 449121..460221
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PH4a_RS02185 (PH4a_02200) pdhR 451710..452480 (-) 771 WP_129041764.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PH4a_RS02190 (PH4a_02205) ampD 452808..453350 (-) 543 WP_129037422.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PH4a_RS02195 (PH4a_02210) ppdD 453567..454028 (+) 462 WP_129037424.1 prepilin peptidase-dependent pilin -
  PH4a_RS02200 (PH4a_02215) pilB 454121..455221 (+) 1101 WP_129037426.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  PH4a_RS02205 (PH4a_02220) - 455218..456417 (+) 1200 WP_129037427.1 type II secretion system F family protein -
  PH4a_RS02210 (PH4a_02225) coaE 456652..457275 (+) 624 WP_129037429.1 dephospho-CoA kinase -
  PH4a_RS02215 (PH4a_02230) zapD 457268..458020 (+) 753 WP_129037430.1 cell division protein ZapD -
  PH4a_RS02220 (PH4a_02235) yacG 458090..458308 (+) 219 WP_129037432.1 DNA gyrase inhibitor YacG -
  PH4a_RS02225 (PH4a_02240) mutT 458369..458761 (-) 393 WP_164971557.1 8-oxo-dGTP diphosphatase MutT -
  PH4a_RS02230 (PH4a_02245) - 458868..460118 (-) 1251 WP_129037436.1 aromatic amino acid transporter -

Sequence

Protein


Download         Length: 366 a.a.        Molecular weight: 41310.70 Da        Isoelectric Point: 7.8327

>NTDB_id=268916 PH4a_RS02200 WP_129037426.1 454121..455221(+) (pilB) [Proteus hauseri strain 15H5D-4a]
MNTAHTATEFIEQILQKSISQRVSDLHFEPQSKGFRIRARIDNHLYLFPSPSNNCSSEIITRLKILADLNIAEKRLPQDG
QFNWFYDEKSYSIRIATLPTLYGEKAVLRLINNLQQPELNKLGFQPVHLILLKKYLTSPQGMILVTGPTGSGKTLTLYSC
LQYLNKDNHNINSVEDPIELPLDGINQTQISEKAGITFATILRALLRQDPDVIMVGEIRDQHTAEIAIKAAQTGHLVLST
LHTNSTRSTLMRLHNLGIAEDLIYSCVNLIISQRLVRCLCQHCKTRLPDKQTVHLRDKVICLPHWQANGCQYCFSGYIGR
TAIYDCLELAKQKLPSPFALFSSGIELIKQGITSLEEVYQVVGDIE

Nucleotide


Download         Length: 1101 bp        

>NTDB_id=268916 PH4a_RS02200 WP_129037426.1 454121..455221(+) (pilB) [Proteus hauseri strain 15H5D-4a]
ATGAATACAGCCCATACTGCAACAGAATTTATTGAACAAATATTACAAAAAAGTATTTCACAACGTGTTTCAGATTTACA
TTTTGAACCTCAAAGTAAGGGGTTTAGAATACGCGCAAGAATAGATAATCATTTATATCTATTTCCATCACCATCAAATA
ACTGTTCTAGTGAAATAATTACCCGTTTAAAAATACTCGCTGATTTAAATATTGCAGAAAAGCGATTACCACAAGATGGA
CAATTTAATTGGTTTTATGATGAAAAATCTTATTCCATACGTATTGCAACATTACCTACACTTTATGGAGAAAAAGCCGT
TTTACGATTAATAAATAATTTACAACAACCCGAGCTAAATAAATTAGGTTTTCAGCCAGTTCATTTAATTTTATTAAAAA
AATATCTCACCTCACCACAAGGAATGATTTTAGTCACAGGTCCAACAGGAAGTGGAAAAACACTGACACTATATAGTTGT
CTTCAATATTTAAATAAAGACAACCACAATATAAATAGCGTAGAAGATCCCATTGAATTACCGCTTGATGGCATCAATCA
GACTCAAATTAGCGAAAAAGCAGGCATTACTTTTGCGACAATATTACGTGCATTATTACGCCAAGATCCCGATGTGATAA
TGGTGGGAGAAATAAGAGATCAACACACTGCTGAAATTGCAATTAAAGCAGCGCAAACAGGACATTTGGTATTATCTACA
CTTCATACTAATTCTACTCGTTCCACACTAATGAGGTTACATAACTTAGGTATTGCTGAAGACTTGATCTACAGTTGCGT
AAATTTAATTATTTCGCAGCGATTGGTTCGCTGCTTATGCCAACATTGCAAAACTCGGTTGCCAGATAAACAAACCGTTC
ACTTAAGAGATAAAGTAATCTGTTTGCCTCATTGGCAAGCTAATGGTTGCCAATACTGTTTTTCAGGTTATATCGGCAGA
ACGGCTATTTATGATTGTCTTGAATTAGCAAAACAAAAATTACCGTCACCTTTTGCATTATTCAGTTCTGGTATTGAATT
AATAAAACAAGGCATAACTTCGTTAGAGGAAGTTTATCAAGTTGTGGGAGATATTGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

45.202

100

0.489
  pilB Vibrio parahaemolyticus RIMD 2210633

46.438

100

0.481
  pilB Vibrio campbellii strain DS40M4

45.91

100

0.475
  pilB Vibrio cholerae strain A1552

44.737

100

0.464
  pilB Acinetobacter baylyi ADP1

42.559

100

0.445
  pilB Acinetobacter baumannii D1279779

42.298

100

0.443
  pilF Neisseria gonorrhoeae MS11

42.063

100

0.434
  pilB Haemophilus influenzae 86-028NP

43.611

98.361

0.429
  pilB Haemophilus influenzae Rd KW20

43.49

98.634

0.429
  pilB Glaesserella parasuis strain SC1401

41.555

100

0.423
  pilF Thermus thermophilus HB27

40

100

0.415
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.005

100

0.407
  pilB/pilB1 Synechocystis sp. PCC 6803

37.659

100

0.404
  ctsE Campylobacter jejuni subsp. jejuni 81-176

37.867

100

0.388

Multiple sequence alignment