Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   C2U40_RS18395 Genome accession   NZ_CP026217
Coordinates   3971614..3972723 (+) Length   369 a.a.
NCBI ID   WP_065475661.1    Uniprot ID   -
Organism   Aeromonas sp. ASNIH4     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3966614..3977723
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2U40_RS18360 (C2U40_18365) - 3967238..3967549 (+) 312 WP_181014760.1 hypothetical protein -
  C2U40_RS18365 (C2U40_18370) - 3967598..3968017 (-) 420 WP_103827460.1 DUF4426 domain-containing protein -
  C2U40_RS18370 (C2U40_18375) yggU 3968036..3968335 (-) 300 WP_011707385.1 DUF167 family protein YggU -
  C2U40_RS18375 (C2U40_18380) - 3968335..3968886 (-) 552 WP_016351969.1 YggT family protein -
  C2U40_RS18380 (C2U40_18385) proC 3968927..3969751 (-) 825 WP_103827459.1 pyrroline-5-carboxylate reductase -
  C2U40_RS18385 (C2U40_18390) - 3969810..3970511 (-) 702 WP_103827458.1 YggS family pyridoxal phosphate-dependent enzyme -
  C2U40_RS18390 (C2U40_18395) pilT 3970551..3971585 (+) 1035 WP_011707389.1 type IVa pilus ATPase TapT Machinery gene
  C2U40_RS18395 (C2U40_18400) pilU 3971614..3972723 (+) 1110 WP_065475661.1 type IVa pilus ATPase TapU Machinery gene
  C2U40_RS18400 (C2U40_18405) yaaA 3972755..3973528 (+) 774 WP_103827457.1 peroxide stress protein YaaA -
  C2U40_RS18405 (C2U40_18410) srmB 3973705..3974928 (-) 1224 WP_016351974.1 ATP-dependent RNA helicase SrmB -
  C2U40_RS18410 (C2U40_18415) - 3975057..3975767 (+) 711 WP_103827456.1 tRNA1(Val) (adenine(37)-N6)-methyltransferase -
  C2U40_RS18415 (C2U40_18420) brnQ 3976004..3977266 (+) 1263 WP_233768849.1 branched-chain amino acid transport system II carrier protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41049.19 Da        Isoelectric Point: 6.5035

>NTDB_id=267867 C2U40_RS18395 WP_065475661.1 3971614..3972723(+) (pilU) [Aeromonas sp. ASNIH4]
MNLDALLTELVARKGSDLFITVGTPPTLKVNGHLQSLAEAPLDKARALALVKETLSQEHFERYLHTKEANYAIHREELGR
FRVSAFWQQELPGMVLRRIETRIPTFDELALPPILQEIALAKRGLVLFVGATGAGKSTTQAAMIGYRNQHVDGHILTVED
PVEFVHQHGRSLITQREVGIDTASFDVALKSSLRQAPDVILIGEIRSQETMEFALQFAETGHLCLATLHANNANQALDRI
LHLVPQEKHRQFLFDLSFNLRAIVAQQLLPSLDGRRRCAAFEVLLNTPLITDIIRKGEMHRLKEVMTKSTELGMQTFDQA
LFALFCAGQIGYSEALAHADSANDLRLLIKLSGREQLGAGTLDNVTLDE

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=267867 C2U40_RS18395 WP_065475661.1 3971614..3972723(+) (pilU) [Aeromonas sp. ASNIH4]
ATGAATCTGGATGCCCTGCTAACCGAGCTGGTCGCCCGTAAAGGCTCGGATCTGTTCATCACGGTCGGCACGCCGCCGAC
CCTCAAGGTCAACGGCCATCTGCAGTCGCTGGCAGAAGCCCCCCTCGACAAGGCAAGGGCGCTGGCGCTGGTCAAGGAGA
CCCTGAGCCAGGAGCACTTCGAGCGCTATCTGCACACCAAGGAGGCTAACTACGCCATCCACCGCGAGGAGCTTGGCCGC
TTTCGGGTCAGCGCCTTCTGGCAGCAGGAGCTGCCTGGCATGGTGCTGCGCCGTATCGAGACCCGCATCCCCACCTTCGA
CGAGCTGGCGCTGCCCCCCATCCTGCAGGAGATCGCCCTGGCCAAGCGCGGGCTGGTGCTGTTCGTCGGTGCCACCGGCG
CCGGAAAATCCACCACCCAGGCTGCCATGATCGGCTATCGCAACCAGCATGTGGACGGCCACATCCTGACGGTGGAAGAT
CCGGTGGAGTTCGTGCATCAGCACGGCCGCAGCCTGATCACCCAGCGCGAGGTAGGCATAGACACAGCGTCGTTCGACGT
AGCGCTGAAGAGTTCGTTGCGCCAGGCCCCTGACGTGATCCTGATCGGCGAAATCCGCAGCCAGGAAACCATGGAGTTTG
CCCTCCAGTTTGCCGAGACCGGCCACCTCTGCCTCGCCACCCTGCACGCCAACAATGCCAACCAGGCGCTGGACCGCATC
CTGCACCTGGTCCCTCAGGAGAAACACCGCCAGTTCCTGTTCGATCTCTCCTTCAACCTGCGTGCCATCGTGGCCCAGCA
ACTGCTGCCCAGCCTGGACGGACGCCGTCGCTGTGCGGCCTTCGAGGTGCTGCTCAACACCCCGCTCATCACCGATATCA
TCCGCAAGGGGGAGATGCACCGGCTCAAGGAGGTGATGACCAAGTCCACCGAGCTCGGCATGCAGACCTTCGATCAGGCG
CTGTTCGCCCTGTTCTGTGCCGGCCAAATTGGCTACAGTGAGGCCCTTGCCCATGCCGACTCGGCCAACGACCTGCGACT
GCTGATCAAGCTGTCGGGGCGCGAGCAGCTGGGTGCAGGCACTCTTGACAACGTCACCCTGGATGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.598

99.729

0.604
  pilU Pseudomonas stutzeri DSM 10701

59.544

95.122

0.566
  pilU Acinetobacter baylyi ADP1

57.224

95.664

0.547
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.296

89.702

0.379
  pilT Legionella pneumophila strain Lp02

40.525

92.954

0.377
  pilT Legionella pneumophila strain ERS1305867

40.525

92.954

0.377
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.12

90.515

0.363
  pilT Vibrio cholerae strain A1552

40.12

90.515

0.363
  pilT Acinetobacter baylyi ADP1

39.296

92.412

0.363

Multiple sequence alignment