Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LCU_RS04700 Genome accession   NZ_CP026116
Coordinates   888972..889847 (-) Length   291 a.a.
NCBI ID   WP_039099523.1    Uniprot ID   -
Organism   Latilactobacillus curvatus JCM 1096 = DSM 20019     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 883972..894847
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LCU_RS04670 (LCU_04685) - 885073..886083 (-) 1011 WP_054644024.1 class I SAM-dependent methyltransferase -
  LCU_RS04675 (LCU_04690) - 886208..886531 (-) 324 WP_039099486.1 hypothetical protein -
  LCU_RS04680 (LCU_04695) - 886509..886889 (-) 381 WP_004270914.1 ComGF family competence protein -
  LCU_RS09955 - 886960..887265 (-) 306 WP_056965894.1 hypothetical protein -
  LCU_RS04685 (LCU_04700) - 887252..887644 (-) 393 WP_004270899.1 hypothetical protein -
  LCU_RS04690 (LCU_04705) comGC 887676..887984 (-) 309 WP_004270901.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LCU_RS04695 (LCU_04710) comGB 887981..888982 (-) 1002 WP_056965892.1 type II secretion system F family protein Machinery gene
  LCU_RS04700 (LCU_04715) comGA 888972..889847 (-) 876 WP_039099523.1 competence type IV pilus ATPase ComGA Machinery gene
  LCU_RS04705 (LCU_04720) - 889951..890682 (-) 732 WP_004270897.1 YebC/PmpR family DNA-binding transcriptional regulator -
  LCU_RS04710 (LCU_04725) - 890772..891272 (-) 501 WP_056965890.1 VanZ family protein -
  LCU_RS04715 (LCU_04730) - 891375..891752 (+) 378 WP_056965888.1 hypothetical protein -
  LCU_RS04725 (LCU_04740) - 891933..892268 (-) 336 WP_056965886.1 hypothetical protein -
  LCU_RS04730 (LCU_04745) - 892463..892702 (+) 240 WP_004270892.1 cytochrome b5 domain-containing protein -
  LCU_RS04735 (LCU_04750) - 892793..893806 (-) 1014 WP_004265264.1 YeiH family protein -
  LCU_RS04740 (LCU_04755) - 893915..894742 (+) 828 WP_054644033.1 LysR family transcriptional regulator -

Sequence

Protein


Download         Length: 291 a.a.        Molecular weight: 32042.84 Da        Isoelectric Point: 5.5654

>NTDB_id=267246 LCU_RS04700 WP_039099523.1 888972..889847(-) (comGA) [Latilactobacillus curvatus JCM 1096 = DSM 20019]
MTEVLTTLLQKCATEKAQDLYFRPLSTGWAIIERLATGLKPLQKVTLSEGNQWLNQMKYQAGMDISETRRPQTGRSAIEV
AGELIYLRLATVGDFLNRESLVVRFIYPIGQTYHCDDSQILSRLMQLIQQPGLLLFAGPTGSGKTTSLYYLAEQVMQDKM
VVAIEDPIEIQQPAFLQLQVNEAAGLTYAALLKISLRLRPDTLIIGEIRDLQTAQHAVSAALSGHLVLSTIHARSTTGVM
DRLLDLGISHSQLTACLAGVAYQSLVVDGHNVAAHYELATAQQLEEAVHED

Nucleotide


Download         Length: 876 bp        

>NTDB_id=267246 LCU_RS04700 WP_039099523.1 888972..889847(-) (comGA) [Latilactobacillus curvatus JCM 1096 = DSM 20019]
ATGACCGAAGTATTAACAACCCTTTTGCAAAAGTGCGCAACGGAAAAAGCGCAGGATCTTTATTTTCGACCACTATCGAC
GGGTTGGGCAATTATTGAACGCTTAGCGACAGGACTAAAGCCGTTACAAAAAGTTACTCTGTCAGAAGGCAATCAATGGC
TCAATCAAATGAAGTATCAAGCGGGGATGGATATCAGTGAAACTCGGCGTCCACAGACCGGCCGTTCAGCGATCGAGGTG
GCAGGCGAATTGATTTATTTACGGTTGGCAACCGTGGGGGATTTTTTGAATCGGGAAAGTTTAGTTGTTCGTTTCATTTA
TCCCATTGGGCAGACTTATCATTGCGACGACTCGCAGATTTTAAGTCGTTTAATGCAGTTAATTCAGCAACCAGGATTAT
TGCTATTTGCCGGTCCAACTGGTTCAGGGAAGACAACAAGTTTGTACTATCTTGCGGAACAAGTCATGCAAGATAAGATG
GTGGTTGCGATTGAAGATCCGATAGAGATTCAACAGCCAGCTTTCTTACAGTTACAGGTGAATGAAGCCGCAGGGTTAAC
CTATGCAGCATTGTTGAAAATCAGTTTGCGATTACGGCCTGATACCTTAATTATTGGTGAGATTCGCGACTTACAGACGG
CCCAACACGCGGTTTCTGCGGCGTTGAGCGGTCACTTGGTACTAAGTACGATTCACGCGCGAAGTACAACGGGGGTGATG
GACCGTCTATTAGATCTTGGAATTAGCCACTCACAATTAACCGCTTGTTTAGCGGGTGTTGCCTATCAATCACTAGTGGT
CGACGGCCACAATGTAGCGGCCCACTATGAGTTAGCAACTGCACAACAATTGGAGGAGGCTGTTCATGAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Latilactobacillus sakei subsp. sakei 23K

69.892

95.876

0.67
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

43.015

93.471

0.402
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

42.593

92.784

0.395
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae R6

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

41.573

91.753

0.381
  comGA/cglA/cilD Streptococcus pneumoniae D39

41.573

91.753

0.381
  comGA Lactococcus lactis subsp. cremoris KW2

41.603

90.034

0.375
  comYA Streptococcus mutans UA140

37.113

100

0.371
  comYA Streptococcus mutans UA159

37.113

100

0.371
  comYA Streptococcus gordonii str. Challis substr. CH1

39.194

93.814

0.368

Multiple sequence alignment