Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   C1S73_RS13780 Genome accession   NZ_CP025792
Coordinates   2861818..2862282 (-) Length   154 a.a.
NCBI ID   WP_038870488.1    Uniprot ID   A0A2A2NC91
Organism   Vibrio jasicida 090810c     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2856818..2867282
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1S73_RS13760 coaE 2857322..2857936 (-) 615 WP_038870481.1 dephospho-CoA kinase -
  C1S73_RS13765 pilD 2857937..2858806 (-) 870 WP_038870483.1 A24 family peptidase Machinery gene
  C1S73_RS13770 pilC 2858878..2860101 (-) 1224 WP_038870485.1 type II secretion system F family protein Machinery gene
  C1S73_RS13775 pilB 2860133..2861818 (-) 1686 WP_038870486.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C1S73_RS13780 pilA 2861818..2862282 (-) 465 WP_038870488.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  C1S73_RS13785 nadC 2862547..2863434 (-) 888 WP_038870489.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C1S73_RS13790 ampD 2863527..2864078 (+) 552 WP_038870491.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C1S73_RS13795 pdhR 2864484..2865251 (+) 768 WP_038870493.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 15915.03 Da        Isoelectric Point: 4.2696

>NTDB_id=263805 C1S73_RS13780 WP_038870488.1 2861818..2862282(-) (pilA) [Vibrio jasicida 090810c]
MKTNKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLAEFPKAAAAAKLAVELCAHENASDSATFITNCTSGD
NGVPATFTLNDIEITVDTAANAGVSGAIAVIAEASAVKGPIAAGEQYVMAATYLAEGLEWDSSCSSGSEDYCPN

Nucleotide


Download         Length: 465 bp        

>NTDB_id=263805 C1S73_RS13780 WP_038870488.1 2861818..2862282(-) (pilA) [Vibrio jasicida 090810c]
ATGAAAACGAATAAACAGAAGAAGCAGCAAGGTTTTACGCTGATTGAATTAATGATTGTGGTGGCGATTATTGGCATTTT
GGCAGCGTTTGCAGTACCTGCATATCAGAACTACACGAAAAAGGCGACTTTAGCTGAATTTCCAAAAGCAGCAGCGGCAG
CTAAACTTGCTGTCGAACTGTGTGCTCATGAAAATGCATCTGACTCTGCTACATTTATTACAAACTGTACTTCAGGAGAC
AACGGTGTCCCAGCTACATTCACATTAAACGATATTGAAATCACTGTGGACACAGCGGCAAATGCAGGTGTTTCAGGCGC
TATAGCAGTAATTGCTGAAGCTTCGGCGGTAAAAGGCCCTATTGCAGCAGGAGAACAATATGTGATGGCCGCAACTTATT
TAGCTGAGGGATTGGAGTGGGACTCGTCATGTTCTTCTGGCTCTGAAGATTACTGCCCTAATTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A2NC91

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

58.599

100

0.597
  pilA Haemophilus influenzae 86-028NP

42.254

92.208

0.39
  pilA Haemophilus influenzae Rd KW20

41.259

92.857

0.383
  pilA2 Legionella pneumophila str. Paris

41.549

92.208

0.383
  pilA2 Legionella pneumophila strain ERS1305867

40.845

92.208

0.377
  pilA Ralstonia pseudosolanacearum GMI1000

41.176

88.312

0.364
  pilA Glaesserella parasuis strain SC1401

41.481

87.662

0.364

Multiple sequence alignment