Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C1S73_RS13775 Genome accession   NZ_CP025792
Coordinates   2860133..2861818 (-) Length   561 a.a.
NCBI ID   WP_038870486.1    Uniprot ID   A0A2A2NCX3
Organism   Vibrio jasicida 090810c     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2855133..2866818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C1S73_RS13745 rplS 2855616..2855969 (+) 354 WP_038870475.1 50S ribosomal protein L19 -
  C1S73_RS13750 yacG 2856295..2856489 (-) 195 WP_038870477.1 DNA gyrase inhibitor YacG -
  C1S73_RS13755 zapD 2856554..2857294 (-) 741 WP_038870479.1 cell division protein ZapD -
  C1S73_RS13760 coaE 2857322..2857936 (-) 615 WP_038870481.1 dephospho-CoA kinase -
  C1S73_RS13765 pilD 2857937..2858806 (-) 870 WP_038870483.1 A24 family peptidase Machinery gene
  C1S73_RS13770 pilC 2858878..2860101 (-) 1224 WP_038870485.1 type II secretion system F family protein Machinery gene
  C1S73_RS13775 pilB 2860133..2861818 (-) 1686 WP_038870486.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C1S73_RS13780 pilA 2861818..2862282 (-) 465 WP_038870488.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  C1S73_RS13785 nadC 2862547..2863434 (-) 888 WP_038870489.1 carboxylating nicotinate-nucleotide diphosphorylase -
  C1S73_RS13790 ampD 2863527..2864078 (+) 552 WP_038870491.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  C1S73_RS13795 pdhR 2864484..2865251 (+) 768 WP_038870493.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61968.77 Da        Isoelectric Point: 5.3987

>NTDB_id=263804 C1S73_RS13775 WP_038870486.1 2860133..2861818(-) (pilB) [Vibrio jasicida 090810c]
MLTNLSTVLRQAGILTFSQEELLLEQAKASGISMPEALLNSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRSSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELSAAIRRLYGRALGQEKSGLKEINQDELAG
LVDIGADEIDNIEDLSQDEAPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILVESQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLNIDRLGYSDEQKQLYLDALKRPQGMI
LMTGPTGSGKTVSLYTGLSLLNKTEINISTAEDPVEINLPGINQVQVQPKIGFGFSEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPQCKQPQEPNAQLQHIGITNTEH
VFKANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGASVHELEKLAIAKGMQTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=263804 C1S73_RS13775 WP_038870486.1 2860133..2861818(-) (pilB) [Vibrio jasicida 090810c]
ATGCTAACCAACCTCTCAACAGTTCTTCGCCAAGCAGGAATACTGACCTTTTCCCAAGAAGAACTGTTACTTGAGCAAGC
TAAAGCTTCTGGTATTTCGATGCCGGAGGCTTTGCTCAATTCTGGCTTTTTCCAATCCCATGAATTGACGGAACACTTAA
GCTCTGTCTTTGGGCTTTCTTGCACCTCTCTCAATCAGTACGAATACGCTTCGCTCTGCCAAACGCTGGGCTTACGTGAT
CTGATCACTCGCCATAATGCTCTGCCACTCAACCGCAGTTCTTCAACTCTGATCTTGGCGGTAGCTGATCCGACTAATCT
CCAAGCTGAAGACGACTTTCGTTTTGCGACAGGACTACAGGTTGAGCTTGTTCTTGCGGATTTTCGTGAACTCAGTGCGG
CAATTAGGCGCTTATATGGTCGAGCACTAGGACAGGAAAAGTCCGGATTAAAAGAGATCAACCAAGATGAACTGGCAGGT
CTGGTTGATATTGGTGCAGATGAAATCGACAACATCGAAGACCTCAGCCAAGACGAAGCACCTGTCAGTCGTTACATTAA
TCAGATTTTGTTGGATGCCGTTCGTAAAGGCGCTTCGGATATTCATTTTGAACCCTACGAAAACATGTATCGAGTGCGCT
TACGTTGTGATGGTATTCTCGTTGAAAGCCAACAACCACCGAGTCATTTAAGTCGTCGCTTATCAGCGCGTATTAAAATC
TTATCCAAACTTGATATCGCCGAACGACGCTTACCACAAGATGGTCGTATCAAGCTTAAGCTAAACCAAGATACTGCAAT
TGATATGCGTGTATCGACATTACCAACGCTATTTGGCGAAAAGATCGTACTGCGTTTGCTCGACAGTAGCTCCGCCTCGC
TAAATATTGACCGCTTGGGTTACAGTGATGAGCAGAAGCAACTTTATCTGGATGCGCTAAAACGGCCACAAGGTATGATT
TTAATGACTGGCCCGACGGGAAGTGGCAAAACCGTCTCGCTCTATACAGGCTTAAGTCTTCTAAACAAAACAGAGATCAA
TATCTCTACAGCGGAAGACCCCGTTGAAATTAACCTGCCTGGCATCAACCAAGTGCAAGTCCAGCCCAAAATTGGCTTTG
GTTTTTCTGAAGCACTTCGTTCTTTCTTACGTCAGGATCCTGATGTGGTCATGGTGGGTGAGATCCGCGATCTAGACACG
GCAGAAATCGCTATTAAAGCCTCACAAACTGGCCACTTAGTTCTGTCTACTCTGCACACCAACTCTGCCGCTGAAACCGT
GATTCGCTTATCCAATATGGGGGTTGAGAGCTTTAACCTTGCTTCATCATTGAGCTTGATCATCGCTCAACGCTTGGCAA
GAAAGCTATGCCCTCAGTGCAAACAACCTCAAGAGCCGAATGCCCAACTGCAACATATTGGGATTACCAACACAGAGCAT
GTCTTTAAGGCCAATCCAGATGGTTGTAACGAGTGTACCCACGGTTATTCAGGTCGAACGGGCATCTATGAAGTGATGAA
GTTTGATGAGTCCCTATCTGAGGCGCTCATAAAAGGAGCTTCAGTACACGAACTAGAAAAACTCGCCATCGCCAAAGGGA
TGCAGACACTACAAATGTCTGGTATAGAAAAACTCAAACAAGGCATCACCAGCTTTAGCGAATTGCAACGCGTTCTCTAC
TTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2A2NCX3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

94.652

100

0.947
  pilB Vibrio parahaemolyticus RIMD 2210633

88.948

100

0.889
  pilB Vibrio cholerae strain A1552

73.132

100

0.733
  pilB Legionella pneumophila strain ERS1305867

49.908

96.791

0.483
  pilB Acinetobacter baumannii D1279779

51.056

92.87

0.474
  pilB Acinetobacter baylyi ADP1

49.077

96.613

0.474
  pilF Neisseria gonorrhoeae MS11

44.149

100

0.444
  pilB/pilB1 Synechocystis sp. PCC 6803

37.542

100

0.398
  pilF Thermus thermophilus HB27

36.786

99.822

0.367

Multiple sequence alignment