Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   MLG_RS15025 Genome accession   NC_008340
Coordinates   284584..285000 (+) Length   138 a.a.
NCBI ID   WP_041717857.1    Uniprot ID   -
Organism   Alkalilimnicola ehrlichii MLHE-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 279584..290000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLG_RS01230 (Mlg_0246) - 279764..282094 (-) 2331 WP_011627997.1 NADP-dependent malic enzyme -
  MLG_RS01235 (Mlg_0247) - 282362..282646 (-) 285 WP_011627998.1 hypothetical protein -
  MLG_RS01240 - 282645..283163 (+) 519 WP_041717856.1 hypothetical protein -
  MLG_RS01245 (Mlg_0248) trxB 283255..284214 (+) 960 WP_011627999.1 thioredoxin-disulfide reductase -
  MLG_RS15025 (Mlg_0249) pilA 284584..285000 (+) 417 WP_041717857.1 pilin Machinery gene
  MLG_RS01255 (Mlg_0250) mntA 285166..285579 (-) 414 WP_011628001.1 type VII toxin-antitoxin system MntA family adenylyltransferase antitoxin -
  MLG_RS01260 (Mlg_0251) - 285637..285939 (+) 303 WP_049753498.1 hypothetical protein -
  MLG_RS01265 (Mlg_0252) - 285949..286386 (+) 438 WP_011628003.1 pilin -
  MLG_RS15030 (Mlg_0253) - 286695..287201 (+) 507 WP_011628004.1 GspH/FimT family pseudopilin -
  MLG_RS01270 (Mlg_0254) pilV 287206..287667 (+) 462 WP_083761810.1 type IV pilus modification protein PilV -
  MLG_RS01275 (Mlg_0255) - 287676..288356 (+) 681 WP_011628006.1 PilW family protein -
  MLG_RS14630 (Mlg_0256) - 288421..289653 (+) 1233 WP_011628007.1 pilus assembly PilX N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 138 a.a.        Molecular weight: 14836.47 Da        Isoelectric Point: 3.8666

>NTDB_id=26304 MLG_RS15025 WP_041717857.1 284584..285000(+) (pilA) [Alkalilimnicola ehrlichii MLHE-1]
MQKQQGFTLIELMIVVAIIGILAAIAIPQYQNYVTRSQASSALSTLSSLRTSFEEAIQRGVDPSFNSEDAGFIGMASDEP
DIGTLSFRADDGGLDFDFSTEATADLQNGTLSLVRTAQGSWSCQSDGIEDRFLPRGCD

Nucleotide


Download         Length: 417 bp        

>NTDB_id=26304 MLG_RS15025 WP_041717857.1 284584..285000(+) (pilA) [Alkalilimnicola ehrlichii MLHE-1]
ATGCAAAAGCAACAGGGTTTTACTTTGATCGAGCTGATGATCGTGGTGGCGATCATCGGTATTTTGGCGGCGATTGCGAT
TCCGCAGTATCAGAATTATGTTACTCGGTCTCAGGCATCTTCGGCGTTGAGCACGCTCTCTTCTCTGAGGACCTCATTTG
AGGAGGCTATTCAACGAGGAGTGGACCCTTCATTTAATTCAGAGGATGCTGGTTTTATCGGCATGGCAAGTGACGAACCG
GACATCGGTACGCTGTCTTTCAGAGCGGACGACGGTGGTCTTGATTTTGACTTTAGTACTGAGGCCACTGCTGATCTTCA
AAACGGAACATTGTCACTCGTAAGAACTGCACAAGGTTCTTGGAGCTGCCAGAGCGATGGTATAGAAGACCGTTTCCTCC
CGCGGGGCTGTGACTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

48.322

100

0.522
  pilA Acinetobacter baumannii strain A118

44.203

100

0.442
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.776

97.101

0.435
  pilA/pilAI Pseudomonas stutzeri DSM 10701

43.382

98.551

0.428
  pilA Vibrio cholerae C6706

38.889

100

0.406
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

38.889

100

0.406
  pilA Vibrio cholerae strain A1552

38.889

100

0.406
  pilA/pilA1 Eikenella corrodens VA1

36.735

100

0.391
  pilA Vibrio parahaemolyticus RIMD 2210633

43.902

89.13

0.391
  comP Acinetobacter baylyi ADP1

33.775

100

0.37
  comF Acinetobacter baylyi ADP1

38.462

94.203

0.362

Multiple sequence alignment