Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MLG_RS01745 Genome accession   NC_008340
Coordinates   386451..387608 (+) Length   385 a.a.
NCBI ID   WP_011628094.1    Uniprot ID   Q0ABT9
Organism   Alkalilimnicola ehrlichii MLHE-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 381451..392608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MLG_RS01720 (Mlg_0338) - 381896..383050 (-) 1155 WP_011628089.1 homoserine O-acetyltransferase -
  MLG_RS01725 (Mlg_0339) - 383130..383708 (-) 579 WP_011628090.1 YggT family protein -
  MLG_RS01730 (Mlg_0340) proC 383708..384535 (-) 828 WP_011628091.1 pyrroline-5-carboxylate reductase -
  MLG_RS01735 (Mlg_0341) - 384532..385245 (-) 714 WP_011628092.1 YggS family pyridoxal phosphate-dependent enzyme -
  MLG_RS01740 (Mlg_0342) pilT 385353..386390 (+) 1038 WP_011628093.1 type IV pilus twitching motility protein PilT Machinery gene
  MLG_RS01745 (Mlg_0343) pilU 386451..387608 (+) 1158 WP_011628094.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MLG_RS01750 (Mlg_0344) - 387868..388584 (-) 717 WP_011628095.1 dihydroorotate dehydrogenase -
  MLG_RS01755 (Mlg_0345) - 388568..389848 (-) 1281 WP_011628096.1 dihydroorotase -
  MLG_RS01760 (Mlg_0346) - 389845..390810 (-) 966 WP_011628097.1 aspartate carbamoyltransferase catalytic subunit -
  MLG_RS01765 (Mlg_0347) pyrR 390807..391316 (-) 510 WP_011628098.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  MLG_RS01770 (Mlg_0348) ruvX 391313..391747 (-) 435 WP_011628099.1 Holliday junction resolvase RuvX -
  MLG_RS01775 (Mlg_0349) - 391740..392315 (-) 576 WP_011628100.1 YqgE/AlgH family protein -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 42967.24 Da        Isoelectric Point: 6.5765

>NTDB_id=26286 MLG_RS01745 WP_011628094.1 386451..387608(+) (pilU) [Alkalilimnicola ehrlichii MLHE-1]
MDFNALLELMVKKGGSDLFITTGVPPCMKVSGQIMPVSKTPLTADQSRQVVQSIMTPAQREEFEQFHECNFAISASGLGR
FRVSAFYQRSTPGMVLRRIETNIPTVEQLKLPKLIEQLAMTKRGLIIFVGGTGTGKSTSLAAMIGYRNRNASGHIITIED
PIEFVHQHNKCIVTQREVGVDTESFDVALRNTLRQAPDVILIGEIRSKETMDYAIAFAETGHLCLATLHANNANQAMDRI
VNFFPSDRLKQLYMDLSLNLKAVVAQQLIPTVDGQGRVPAVEVLLGTPLVHDKIRKGEVHELKDIMKRSNEQGMRTFDQH
LYELYRNGQITYEDALRHADSENEVRLMAKLHESGEAPQASLEKNTEGLSLVDDEEEAPRGPKLR

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=26286 MLG_RS01745 WP_011628094.1 386451..387608(+) (pilU) [Alkalilimnicola ehrlichii MLHE-1]
ATGGATTTCAACGCCCTGCTCGAACTCATGGTGAAGAAAGGGGGCTCGGACCTGTTCATCACCACGGGTGTGCCGCCCTG
CATGAAGGTCTCCGGGCAGATCATGCCGGTCTCCAAGACACCGCTCACCGCCGACCAGTCGCGTCAGGTGGTGCAATCGA
TTATGACCCCCGCCCAGCGCGAGGAGTTTGAGCAGTTCCACGAATGCAACTTTGCCATCTCCGCCAGCGGTTTGGGGCGG
TTCCGGGTCAGCGCCTTCTATCAGCGCTCGACACCGGGCATGGTGCTGCGCCGGATCGAGACCAATATCCCGACGGTGGA
GCAGCTCAAGCTGCCGAAGCTCATCGAGCAGCTGGCCATGACCAAGCGGGGGCTGATCATCTTCGTCGGCGGCACCGGCA
CCGGCAAATCCACCTCGCTGGCCGCGATGATTGGCTATCGTAACCGCAACGCCTCGGGCCACATCATCACCATCGAGGAT
CCGATCGAGTTCGTCCACCAGCACAACAAGTGCATCGTCACCCAGCGCGAGGTGGGGGTGGACACTGAATCCTTCGACGT
GGCCCTGCGCAACACCCTGCGCCAGGCCCCGGACGTGATCCTGATCGGCGAGATCCGCTCCAAGGAGACCATGGATTACG
CCATTGCCTTCGCCGAGACGGGGCATCTTTGCCTGGCGACGCTGCACGCCAACAACGCCAACCAGGCGATGGACCGCATC
GTCAACTTCTTCCCTTCCGACCGGCTCAAGCAGCTTTACATGGACCTGTCGCTGAACCTCAAGGCGGTGGTGGCGCAGCA
GTTGATCCCGACAGTGGACGGCCAGGGCCGGGTGCCGGCGGTGGAGGTCCTGCTGGGTACGCCGCTGGTGCACGACAAGA
TCCGCAAGGGCGAGGTGCACGAGCTCAAGGACATCATGAAGCGCTCCAACGAGCAGGGCATGCGCACCTTCGACCAGCAC
CTCTACGAGCTCTACCGCAACGGCCAGATCACCTACGAGGATGCGCTGCGCCACGCCGACTCGGAGAACGAGGTGCGGCT
GATGGCCAAGCTGCACGAAAGCGGCGAGGCGCCGCAGGCGTCGCTGGAGAAGAACACGGAGGGGCTTTCGCTGGTGGACG
ATGAGGAGGAGGCGCCCCGCGGCCCCAAACTGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(107-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q0ABT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

68.684

98.701

0.678
  pilU Acinetobacter baylyi ADP1

62.5

95.584

0.597
  pilU Vibrio cholerae strain A1552

54.821

94.286

0.517
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.38

92.208

0.4
  pilT Legionella pneumophila strain Lp02

42.09

87.013

0.366
  pilT Legionella pneumophila strain ERS1305867

42.09

87.013

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

42.216

86.753

0.366
  pilT Vibrio cholerae strain A1552

42.216

86.753

0.366
  pilT Pseudomonas aeruginosa PAK

41.003

88.052

0.361

Multiple sequence alignment