Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   SHEWMR4_RS02230 Genome accession   NC_008321
Coordinates   463685..464950 (-) Length   421 a.a.
NCBI ID   WP_011621222.1    Uniprot ID   -
Organism   Shewanella sp. MR-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 458685..469950
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SHEWMR4_RS02195 (Shewmr4_0412) - 458811..459737 (+) 927 WP_011621215.1 exopolyphosphatase -
  SHEWMR4_RS02200 (Shewmr4_0413) - 459811..460347 (-) 537 WP_011621216.1 thioredoxin family protein -
  SHEWMR4_RS02205 (Shewmr4_0414) mutT 460496..460894 (-) 399 WP_011621217.1 8-oxo-dGTP diphosphatase MutT -
  SHEWMR4_RS02210 (Shewmr4_0415) yacG 460939..461148 (-) 210 WP_011621218.1 DNA gyrase inhibitor YacG -
  SHEWMR4_RS02215 (Shewmr4_0416) zapD 461271..462005 (-) 735 WP_011621219.1 cell division protein ZapD -
  SHEWMR4_RS02220 (Shewmr4_0417) coaE 461998..462615 (-) 618 WP_011621220.1 dephospho-CoA kinase -
  SHEWMR4_RS02225 (Shewmr4_0418) pilD 462703..463629 (-) 927 WP_011621221.1 prepilin peptidase Machinery gene
  SHEWMR4_RS02230 (Shewmr4_0419) pilC 463685..464950 (-) 1266 WP_011621222.1 type II secretion system F family protein Machinery gene
  SHEWMR4_RS02235 (Shewmr4_0420) pilB 465080..466789 (-) 1710 WP_011621223.1 type IV-A pilus assembly ATPase PilB Machinery gene
  SHEWMR4_RS02240 (Shewmr4_0421) - 466942..468294 (+) 1353 WP_041408676.1 O-antigen ligase family protein -
  SHEWMR4_RS02245 (Shewmr4_0422) - 468345..468716 (-) 372 WP_011621225.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 421 a.a.        Molecular weight: 46176.47 Da        Isoelectric Point: 10.0949

>NTDB_id=26262 SHEWMR4_RS02230 WP_011621222.1 463685..464950(-) (pilC) [Shewanella sp. MR-4]
MATATTVKKPRAKKNEQKSQPKIYTFEWKGLNRDGKKTGGELKGTSVAEVKSQLKLQGVNPKVVRKKASPLFAHNPDIKP
MDIAMVTRQIATMLAAGVPLVTTIEMLGRGHEKQKMRELLGTILSEVQSGIPLSDSLRPHRRYFDDLYVDLVAAGEHSGS
LDAVFDRIATYREKAEALKSKIKKAMFYPAAVVVVAIAVTTLLLLFVVPQFESIFASFGAELPAFTQMIVGISRFLQSSW
YIFFAAIAISIWLFVRAHRNSQMFRDRIDELVLKIPIIGEILHKAAMARFSRTLATTFAAGVPLIDGMESAAGASGNAVY
RSALLKVRQEVMAGMQMNVAMRTTGLFPDMLIQMVMIGEESGSLDSMLNKVANIYEMQVDDAVDGLSSLIEPIMMVVIGI
LVGGLIVGMYLPIFQMGNVVG

Nucleotide


Download         Length: 1266 bp        

>NTDB_id=26262 SHEWMR4_RS02230 WP_011621222.1 463685..464950(-) (pilC) [Shewanella sp. MR-4]
ATGGCTACGGCAACGACAGTAAAAAAGCCTCGTGCCAAAAAGAACGAGCAAAAAAGCCAACCTAAGATCTATACCTTTGA
GTGGAAGGGCTTAAACCGTGATGGCAAAAAAACCGGTGGCGAATTAAAAGGCACCAGTGTCGCCGAGGTGAAAAGTCAGC
TTAAGCTCCAAGGGGTAAACCCTAAAGTTGTACGCAAAAAAGCATCACCTCTTTTCGCTCACAATCCCGATATCAAACCT
ATGGATATCGCCATGGTAACACGCCAAATAGCGACTATGCTGGCAGCAGGTGTCCCCTTAGTCACCACAATAGAAATGCT
CGGCCGCGGCCATGAAAAGCAAAAAATGCGCGAGTTACTCGGTACTATATTGTCAGAAGTACAATCGGGTATTCCACTAT
CAGACTCACTACGCCCCCATAGACGTTATTTTGATGATCTCTACGTCGATCTTGTCGCAGCGGGTGAACATTCAGGCTCT
CTTGATGCAGTGTTTGACCGAATTGCAACATACAGAGAAAAAGCCGAAGCCTTAAAGTCGAAAATTAAAAAGGCGATGTT
TTACCCTGCCGCAGTCGTTGTGGTAGCGATAGCAGTAACAACACTGCTTCTGTTGTTTGTTGTACCGCAATTTGAATCAA
TTTTTGCAAGCTTTGGTGCAGAGCTTCCAGCTTTTACCCAGATGATCGTTGGCATTTCGCGTTTTTTACAGAGTTCTTGG
TATATATTCTTCGCAGCCATAGCTATCAGTATCTGGTTATTTGTCCGTGCCCACCGCAATTCACAAATGTTTAGGGATCG
AATAGATGAACTTGTGCTTAAGATTCCCATTATCGGTGAGATATTGCATAAAGCAGCCATGGCACGATTTTCCCGCACCT
TAGCCACCACCTTTGCGGCAGGGGTGCCTTTAATCGATGGTATGGAGTCAGCTGCCGGCGCCTCTGGCAACGCGGTTTAT
CGATCGGCACTATTGAAAGTACGCCAAGAAGTGATGGCTGGGATGCAGATGAACGTCGCCATGCGTACCACAGGGCTTTT
CCCCGATATGCTGATCCAAATGGTGATGATCGGTGAAGAGTCTGGCTCACTCGATAGTATGCTCAATAAAGTAGCCAATA
TTTATGAAATGCAGGTCGATGATGCCGTTGATGGCTTATCAAGCCTTATCGAGCCCATTATGATGGTAGTCATTGGCATC
TTGGTCGGCGGTTTGATTGTCGGTATGTATCTACCGATTTTCCAAATGGGTAATGTCGTAGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(290-412)

Type II secretion system (T2SS), protein F (T2SSF)

(87-209)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.456

93.824

0.53
  pilC Acinetobacter baylyi ADP1

51.637

94.299

0.487
  pilC Acinetobacter baumannii D1279779

51.256

94.537

0.485
  pilC Legionella pneumophila strain ERS1305867

49.505

95.962

0.475
  pilC Vibrio cholerae strain A1552

46.366

94.774

0.439
  pilC Vibrio campbellii strain DS40M4

43.672

95.724

0.418
  pilG Neisseria meningitidis 44/76-A

41.542

95.487

0.397
  pilG Neisseria gonorrhoeae MS11

41.542

95.487

0.397

Multiple sequence alignment