Detailed information NTG0026261

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: SHEWMR4_RS02225
• Genome accession: NC_008321
• Coordinates: 462703..463629 (-)
• Length: 308 a.a.
• NCBI ID: WP_011621221.1
• Uniprot ID: -
• Organism: Shewanella sp. MR-4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 457703..468629

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SHEWMR4_RS02195 (Shewmr4_0412)	-	458811..459737 (+)	927	WP_011621215.1	exopolyphosphatase	-
SHEWMR4_RS02200 (Shewmr4_0413)	-	459811..460347 (-)	537	WP_011621216.1	thioredoxin family protein	-
SHEWMR4_RS02205 (Shewmr4_0414)	mutT	460496..460894 (-)	399	WP_011621217.1	8-oxo-dGTP diphosphatase MutT	-
SHEWMR4_RS02210 (Shewmr4_0415)	yacG	460939..461148 (-)	210	WP_011621218.1	DNA gyrase inhibitor YacG	-
SHEWMR4_RS02215 (Shewmr4_0416)	zapD	461271..462005 (-)	735	WP_011621219.1	cell division protein ZapD	-
SHEWMR4_RS02220 (Shewmr4_0417)	coaE	461998..462615 (-)	618	WP_011621220.1	dephospho-CoA kinase	-
SHEWMR4_RS02225 (Shewmr4_0418)	pilD	462703..463629 (-)	927	WP_011621221.1	prepilin peptidase	Machinery gene
SHEWMR4_RS02230 (Shewmr4_0419)	pilC	463685..464950 (-)	1266	WP_011621222.1	type II secretion system F family protein	Machinery gene
SHEWMR4_RS02235 (Shewmr4_0420)	pilB	465080..466789 (-)	1710	WP_011621223.1	type IV-A pilus assembly ATPase PilB	Machinery gene
SHEWMR4_RS02240 (Shewmr4_0421)	-	466942..468294 (+)	1353	WP_041408676.1	O-antigen ligase family protein	-

Sequence

Protein

• Length: 308 a.a.
• Molecular weight: 34302.80 Da
• Isoelectric Point: 7.3447

>NTDB_id=26261 SHEWMR4_RS02225 WP_011621221.1 462703..463629(-) (pilD) [Shewanella sp. MR-4]
MSDFISLLSHSLAQSPWLFIALSFVFAATIGSFLNVVIHRFPVMMKREWQQECNQYLQEYHVDVVKQIGIEKLNKPIDTY
PEKYNLVVPGSACPKCKTAIKPWHNLPIVGWLMLRGKCAACDAPISSRYPIIELVTGLLVATLAWHFGPSWQFVFAAVLT
FVLIALTGIDLDEMLLPDQMTLPLLWLGLLINLNHTFTTPTDAVIGAAAGYLSLWSVFWLFKLLTGKEGMGYGDFKLLAV
FGAWLGWQMLPLVILLSSLVGALVGITLIVLKRNQLANPIPFGPYIAAAGWIALIWGQPIVDWYLSTL

Nucleotide

• Length: 927 bp

>NTDB_id=26261 SHEWMR4_RS02225 WP_011621221.1 462703..463629(-) (pilD) [Shewanella sp. MR-4]
ATGTCAGACTTTATTTCTCTTTTAAGCCACTCTCTTGCGCAGAGTCCTTGGCTATTTATTGCGTTAAGTTTTGTGTTTGC
TGCCACTATTGGTAGCTTTTTAAATGTCGTCATTCACCGCTTTCCGGTAATGATGAAGCGCGAATGGCAACAGGAGTGTA
ATCAATATCTGCAAGAATACCATGTAGATGTGGTAAAGCAGATTGGTATCGAAAAGCTCAATAAGCCGATAGATACTTAC
CCTGAAAAATACAATCTAGTAGTACCAGGTTCCGCCTGCCCTAAATGTAAAACCGCAATCAAACCTTGGCATAATCTGCC
GATTGTGGGCTGGTTAATGCTCAGAGGAAAATGCGCTGCCTGCGATGCACCAATATCCTCACGCTATCCAATTATTGAGT
TAGTAACAGGGTTACTCGTTGCAACGCTCGCTTGGCATTTTGGTCCTAGCTGGCAATTTGTGTTTGCTGCGGTATTAACC
TTTGTATTGATTGCATTAACGGGTATCGATCTGGATGAGATGCTGCTTCCCGACCAGATGACCCTTCCACTCCTATGGCT
AGGATTACTCATTAACCTCAATCATACCTTTACCACGCCAACCGATGCCGTTATAGGCGCTGCAGCGGGCTATTTGAGTC
TCTGGTCGGTGTTTTGGCTGTTTAAGCTACTCACAGGTAAGGAAGGCATGGGGTATGGCGACTTTAAACTGCTCGCCGTA
TTTGGTGCATGGCTGGGTTGGCAGATGCTCCCCCTAGTCATTTTACTGTCCTCTTTAGTTGGTGCCCTTGTTGGCATCAC
GCTCATCGTGCTCAAACGTAATCAGCTCGCAAACCCTATTCCCTTTGGGCCTTATATTGCCGCCGCTGGGTGGATAGCAT
TGATATGGGGACAACCCATAGTCGATTGGTATCTAAGTACGCTATAA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (158-266)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (25-147)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	51.864	95.779	0.497
pilD	Acinetobacter nosocomialis M2	49.346	99.351	0.49
pilD	Acinetobacter baumannii D1279779	49.02	99.351	0.487
pilD	Vibrio campbellii strain DS40M4	50.68	95.455	0.484
pilD	Neisseria gonorrhoeae MS11	46.048	94.481	0.435