Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   C0J56_RS25400 Genome accession   NZ_CP025542
Coordinates   5639318..5640535 (+) Length   405 a.a.
NCBI ID   WP_109756361.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain 2P24     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5634318..5645535
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0J56_RS25375 (C0J56_25360) - 5635715..5636011 (+) 297 WP_134925860.1 DUF2845 domain-containing protein -
  C0J56_RS25385 (C0J56_25370) - 5636338..5636691 (-) 354 WP_134925862.1 BON domain-containing protein -
  C0J56_RS25390 (C0J56_25375) - 5636972..5637391 (-) 420 WP_134925863.1 pilin -
  C0J56_RS25395 (C0J56_25380) pilB 5637615..5639315 (+) 1701 WP_134925864.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C0J56_RS25400 (C0J56_25385) pilC 5639318..5640535 (+) 1218 WP_109756361.1 type II secretion system F family protein Machinery gene
  C0J56_RS25405 (C0J56_25390) pilD 5640537..5641406 (+) 870 WP_116833472.1 A24 family peptidase Machinery gene
  C0J56_RS25410 (C0J56_25395) coaE 5641406..5642029 (+) 624 WP_134925865.1 dephospho-CoA kinase -
  C0J56_RS25415 (C0J56_25400) yacG 5642026..5642232 (+) 207 WP_109756364.1 DNA gyrase inhibitor YacG -
  C0J56_RS25420 (C0J56_25405) - 5642229..5642444 (-) 216 WP_013694234.1 hypothetical protein -
  C0J56_RS25425 (C0J56_25410) - 5642508..5643194 (-) 687 WP_134925866.1 energy-coupling factor ABC transporter permease -
  C0J56_RS25430 (C0J56_25415) - 5643340..5643966 (+) 627 WP_003205682.1 DUF1780 domain-containing protein -
  C0J56_RS25435 (C0J56_25420) - 5643963..5644490 (+) 528 WP_134925867.1 MOSC domain-containing protein -
  C0J56_RS25440 (C0J56_25425) - 5644543..5644716 (+) 174 WP_003185543.1 DUF3094 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44176.82 Da        Isoelectric Point: 9.8765

>NTDB_id=262463 C0J56_RS25400 WP_109756361.1 5639318..5640535(+) (pilC) [Pseudomonas fluorescens strain 2P24]
MAVKAVKTDVYTWEGKDRKGTKMTGELTGQSPALVKAQLRKQGINPQKVRKKSTSIFSKGKRIKPLDIALFTRQMATMLK
AGVPLLQAFDIIGEGFDNANMRKLVDEVKQEVAAGNSFAASLRKCPQYFDDLYCNLVDAGEQAGALDTLLDRVATYKEKS
EALKAKIKKAMTYPAAVVLVAAVVTGILLVKVVPQFESVFSGFGAQLPAFTVMVIGLSEFMQESWWILLGGMVGGFFGVK
YALRRSEAFRDWRDKWLLKLPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFKRAVQRIKQDVSTGM
QLNFSMRTSGIFPNLAIQMTAIGEESGALDDMLDKVASFYEAEVDNLVDNLTSLMEPFIMVVLGVVVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=262463 C0J56_RS25400 WP_109756361.1 5639318..5640535(+) (pilC) [Pseudomonas fluorescens strain 2P24]
ATGGCGGTCAAAGCAGTCAAAACCGATGTTTACACTTGGGAAGGCAAGGACCGCAAAGGCACGAAAATGACCGGCGAGCT
GACGGGCCAGAGCCCGGCCCTGGTCAAGGCACAGCTGCGCAAGCAAGGCATCAACCCGCAGAAGGTACGCAAGAAGTCCA
CCTCGATATTCAGCAAGGGCAAGCGCATCAAGCCATTGGATATCGCCCTCTTCACCCGCCAGATGGCAACGATGCTCAAG
GCAGGCGTGCCGCTGTTGCAGGCGTTCGACATCATTGGCGAAGGCTTCGACAACGCCAATATGCGCAAGCTGGTGGACGA
GGTGAAACAGGAAGTCGCCGCCGGCAACAGCTTCGCCGCATCGCTGCGCAAGTGCCCGCAGTATTTCGACGACCTGTACT
GCAACCTGGTGGACGCCGGTGAACAGGCCGGCGCCCTGGACACGCTGCTGGACCGGGTCGCGACCTACAAGGAAAAGAGC
GAAGCCCTCAAGGCCAAAATCAAGAAAGCCATGACCTATCCAGCCGCCGTCGTTCTCGTCGCCGCAGTGGTCACGGGGAT
CCTGCTGGTCAAGGTGGTGCCGCAGTTCGAATCGGTATTCTCCGGGTTCGGTGCGCAGCTGCCGGCCTTTACGGTGATGG
TCATCGGCTTGTCAGAGTTCATGCAGGAGTCATGGTGGATACTGCTCGGCGGGATGGTGGGCGGATTCTTTGGCGTGAAA
TACGCGCTCAGGCGCTCCGAGGCCTTTCGTGACTGGCGCGATAAATGGCTGCTCAAGCTGCCACTGATCGGCACCCTGAT
GTACAAATCCGCCGTGGCCCGCTACGCCCGCACGCTCTCCACCACATTCGCCGCCGGCGTGCCGCTGGTGGAAGCCCTGG
ACTCGGTGTCTGGCGCCACCGGCAACGTGGTGTTCAAGCGCGCGGTGCAGCGCATCAAGCAAGACGTCTCGACCGGCATG
CAGTTGAATTTCTCCATGCGTACCTCAGGGATCTTTCCGAACCTGGCGATCCAGATGACCGCCATCGGCGAAGAATCCGG
TGCGCTGGATGACATGCTCGACAAGGTGGCGAGCTTTTATGAGGCCGAGGTGGACAATCTGGTGGACAACCTCACCAGCC
TGATGGAACCCTTCATCATGGTGGTCCTGGGGGTCGTCGTCGGTGGCCTGGTGGTTGCCATGTACTTGCCCATCTTTCAA
CTCGGCTCTGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.062

100

0.751
  pilC Acinetobacter baylyi ADP1

59.102

99.012

0.585
  pilC Acinetobacter baumannii D1279779

57.843

100

0.583
  pilC Legionella pneumophila strain ERS1305867

54.545

97.778

0.533
  pilC Vibrio campbellii strain DS40M4

44.081

98.025

0.432
  pilC Vibrio cholerae strain A1552

43.325

98.025

0.425
  pilG Neisseria gonorrhoeae MS11

42.469

100

0.425
  pilG Neisseria meningitidis 44/76-A

41.337

99.753

0.412
  pilC Thermus thermophilus HB27

37.157

99.012

0.368

Multiple sequence alignment