Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   C0J56_RS25395 Genome accession   NZ_CP025542
Coordinates   5637615..5639315 (+) Length   566 a.a.
NCBI ID   WP_134925864.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain 2P24     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5632615..5644315
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0J56_RS25370 (C0J56_25355) - 5632780..5635713 (+) 2934 WP_209022168.1 DUF748 domain-containing protein -
  C0J56_RS25375 (C0J56_25360) - 5635715..5636011 (+) 297 WP_134925860.1 DUF2845 domain-containing protein -
  C0J56_RS25385 (C0J56_25370) - 5636338..5636691 (-) 354 WP_134925862.1 BON domain-containing protein -
  C0J56_RS25390 (C0J56_25375) - 5636972..5637391 (-) 420 WP_134925863.1 pilin -
  C0J56_RS25395 (C0J56_25380) pilB 5637615..5639315 (+) 1701 WP_134925864.1 type IV-A pilus assembly ATPase PilB Machinery gene
  C0J56_RS25400 (C0J56_25385) pilC 5639318..5640535 (+) 1218 WP_109756361.1 type II secretion system F family protein Machinery gene
  C0J56_RS25405 (C0J56_25390) pilD 5640537..5641406 (+) 870 WP_116833472.1 A24 family peptidase Machinery gene
  C0J56_RS25410 (C0J56_25395) coaE 5641406..5642029 (+) 624 WP_134925865.1 dephospho-CoA kinase -
  C0J56_RS25415 (C0J56_25400) yacG 5642026..5642232 (+) 207 WP_109756364.1 DNA gyrase inhibitor YacG -
  C0J56_RS25420 (C0J56_25405) - 5642229..5642444 (-) 216 WP_013694234.1 hypothetical protein -
  C0J56_RS25425 (C0J56_25410) - 5642508..5643194 (-) 687 WP_134925866.1 energy-coupling factor ABC transporter permease -
  C0J56_RS25430 (C0J56_25415) - 5643340..5643966 (+) 627 WP_003205682.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62725.72 Da        Isoelectric Point: 5.4307

>NTDB_id=262462 C0J56_RS25395 WP_134925864.1 5637615..5639315(+) (pilB) [Pseudomonas fluorescens strain 2P24]
MNDIALSGLTKQLVLAELLTEQSAQQAYQQAQRSRTPLVSYLVQNKLVQSRQVAEIASEHFGVALLDLNSLDKETQPTGL
VSEKLVRQHHALPLWRRGNKLFVGISDPTNHQAINDIQFSTGLTTEAILVEDDKLSDAIEKFFESSSTGLEGMGDVDLDG
LDIESIDDTKQDSIAGQDTDDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKIYRVRVRTDGMLREVARPPIQLANRIAARL
KVMASLDISERRKPQDGRLKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
LILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLIRLQNMGIPGFNIATAVHLIIAQRLARKLCNHCKKAIEIPEETLLKEGFPR
ERIGTFTIYEPVGCEQCNHGYKGRVGVYEVVKNTPELQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKVMQGVTSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=262462 C0J56_RS25395 WP_134925864.1 5637615..5639315(+) (pilB) [Pseudomonas fluorescens strain 2P24]
ATGAATGACATCGCCCTCAGCGGCCTGACCAAGCAATTGGTGCTGGCCGAGCTGCTCACCGAGCAAAGCGCGCAACAGGC
GTATCAACAGGCCCAGCGCAGTCGCACGCCCCTGGTCAGTTATCTGGTACAGAACAAACTGGTCCAGAGCCGCCAGGTCG
CGGAGATCGCCTCGGAGCACTTCGGCGTCGCCCTGCTGGACCTCAACAGCCTGGACAAGGAAACCCAGCCCACGGGCCTG
GTCAGCGAAAAACTGGTGCGCCAACATCACGCCCTGCCGCTGTGGCGACGAGGCAACAAATTGTTCGTGGGCATCTCCGA
CCCCACCAATCACCAGGCCATCAATGACATCCAGTTCAGCACCGGCCTGACCACCGAAGCCATCCTGGTGGAGGACGACA
AGCTCAGCGACGCCATCGAAAAGTTCTTCGAATCCAGTAGCACAGGCCTGGAAGGCATGGGCGATGTCGACCTCGATGGC
CTGGACATCGAATCGATCGACGATACCAAGCAGGATTCCATCGCCGGCCAGGACACTGACGACGCGCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCCTATGAAAAAATCTACCGCG
TGCGGGTACGCACCGACGGCATGCTGCGAGAAGTCGCCAGGCCGCCGATCCAACTGGCCAACCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTCGATATTTCCGAACGACGCAAACCCCAGGACGGGCGCCTGAAAATGCGCCTGTCGAAAAGCAA
ATCCATCGACTTCCGGGTCAACACCCTGCCCACGCTCTGGGGCGAAAAAGTGGTGATCCGGATCCTCGATCCGTCCAGCG
CGCAAATGGGCATCGATGCCCTGGGCTACGAACCGGACCAGAAAGACCTGTACATGGCGGCCCTCAAGCAACCGCAAGGG
CTGATCCTGGTGACCGGCCCGACCGGCTCGGGCAAGACCGTATCGCTGTACACCGGGCTGAACATCCTCAACACCGTGGA
CATCAACATTTCTACCGCTGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTGAACCCACGCCAGG
GGCTGGACTTCGCCCAGGCCCTGCGTTCGTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGGCACCTGGTGCTCTCCACCCTGCACACCAACAGCGCCGCCGA
AACCCTGATCCGCCTGCAGAACATGGGCATCCCAGGGTTCAACATCGCCACCGCCGTGCACCTGATCATCGCCCAGCGGC
TGGCGCGCAAGTTGTGCAACCACTGCAAGAAGGCCATCGAGATTCCCGAGGAGACCTTGCTCAAGGAAGGCTTCCCCCGG
GAACGCATCGGCACATTCACGATCTATGAGCCGGTCGGTTGCGAACAGTGCAACCATGGTTACAAAGGGCGCGTGGGGGT
TTATGAAGTGGTCAAGAACACGCCCGAGCTGCAGCGGTTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATCTGCAAA
TGCGCAAGGACGGTTTCAATGACCTGCGTACATCGGGGCTGCTCAAAGTGATGCAGGGTGTCACCAGCCTCGAAGAAATC
AACCGGGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.127

100

0.581
  pilB Acinetobacter baumannii D1279779

57.447

99.647

0.572
  pilB Legionella pneumophila strain ERS1305867

55.379

100

0.555
  pilB Vibrio cholerae strain A1552

50.619

99.823

0.505
  pilB Vibrio campbellii strain DS40M4

48.596

100

0.489
  pilF Neisseria gonorrhoeae MS11

48.313

99.47

0.481
  pilB Vibrio parahaemolyticus RIMD 2210633

48.921

98.233

0.481
  pilF Thermus thermophilus HB27

36.042

100

0.36

Multiple sequence alignment