Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CU052_RS07535 Genome accession   NZ_CP025537
Coordinates   1571964..1573715 (+) Length   583 a.a.
NCBI ID   WP_005451505.1    Uniprot ID   A0A9X3RXD0
Organism   Vibrio harveyi strain 345     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1566964..1578715
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU052_RS07515 (CU052_07915) pilM 1569263..1570279 (+) 1017 WP_026138410.1 type IV pilus assembly protein PilM Machinery gene
  CU052_RS07520 (CU052_07920) pilN 1570263..1570838 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  CU052_RS07525 (CU052_07925) pilO 1570831..1571424 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  CU052_RS07530 (CU052_07930) pilP 1571414..1571929 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  CU052_RS07535 (CU052_07935) pilQ 1571964..1573715 (+) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  CU052_RS07540 (CU052_07940) aroK 1573905..1574423 (+) 519 WP_005451507.1 shikimate kinase AroK -
  CU052_RS07545 (CU052_07945) aroB 1574455..1575555 (+) 1101 WP_038898114.1 3-dehydroquinate synthase -
  CU052_RS07550 (CU052_07950) - 1575609..1577111 (+) 1503 WP_029789966.1 AAA family ATPase -
  CU052_RS07555 (CU052_07955) - 1577181..1578020 (+) 840 WP_005451513.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 583 a.a.        Molecular weight: 63768.79 Da        Isoelectric Point: 4.5053

>NTDB_id=262397 CU052_RS07535 WP_005451505.1 1571964..1573715(+) (pilQ) [Vibrio harveyi strain 345]
MKMRQGLTLTPRKLTGLFSLWLFLLLAPLVAAEEVKSNSLKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDDQLYLLDVKDFSTVVESVEVFRDTSTTRLVAYIDEDYTHDYRLTGRYLEVKVGKLKANEKAPDKSILEKEGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKDELDLREKQQ
LEKQKLEQEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSDRGSMTIDERTNSLLIRELPENIQVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGVRWGFTSINGSHTVGGSIENNLATIGLYDGGGGDGDGEDGGGSVGIDDFLNVNLAATSPN
ATSIAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTP
QITPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALF
RRSYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1752 bp        

>NTDB_id=262397 CU052_RS07535 WP_005451505.1 1571964..1573715(+) (pilQ) [Vibrio harveyi strain 345]
ATGAAAATGAGACAAGGATTAACACTGACACCAAGGAAGCTAACAGGGCTCTTCTCTTTATGGTTGTTTCTACTTTTAGC
CCCTTTAGTCGCAGCGGAAGAAGTAAAGTCTAACTCGCTTAAGAGCATCGACTTCCGTACTAATAAAGATAAAGACGCGG
TTATCGTCGTAGAGCTTGCATCGCCAGCTGCTATTGTTGATATCAAGCGTGTGCAAGAAGGTTTAAGTATCGACTTGCTC
AATACGTCAGTTAAAGACGATCAGCTTTATCTACTTGATGTAAAAGACTTCTCTACAGTGGTAGAGAGTGTTGAGGTTTT
CCGCGATACCTCTACGACGCGTTTGGTCGCTTACATTGATGAAGATTATACCCATGACTATCGCCTCACAGGACGTTACC
TTGAGGTGAAGGTTGGTAAGCTCAAAGCCAACGAAAAAGCACCAGACAAAAGTATTCTGGAAAAAGAAGGCAAGTTGATT
TCGATTAACTTCCAAGATATTCCGGTGCGAAATGTGCTGCAGCTTATTGCTGATTACAACCAGTTTAACCTTGTGGTTTC
AGACTCAGTTGAAGGGAACCTGACCTTGCGTTTGGACGGTGTACCTTGGCAACAAGTGCTGGATATCATCCTTCAAGTCA
AAGGCTTGGATAAGCGTGTAGATGGTAACGTTATTTTGGTTGCTCCGAAGGATGAGCTCGATCTGCGCGAGAAGCAACAA
CTAGAAAAACAAAAGCTTGAACAAGAAATGGGTGAGCTTTCGTCTGAAATTATCAAAGTGAATTTTGCGAAAGCATCGGA
TATTGCAGAGATGATTAACGGTGAAGGTAACATTAGTATGCTTTCTGATCGTGGCAGCATGACGATTGATGAGCGTACTA
ACTCATTATTGATCCGTGAGTTGCCGGAGAATATTCAAGTAATTCGCGAAATCATTGAATCATTAGATATCCCAGTAAAA
CAAGTTCAGATTGAAGCACGTATCGTGACTATCAATGAAGGTAACATGGATGAACTTGGCGTTCGCTGGGGCTTCACATC
GATTAATGGTAGTCATACGGTTGGTGGATCGATTGAGAATAACCTTGCAACCATTGGTCTCTACGATGGCGGCGGTGGCG
ACGGCGATGGTGAAGACGGTGGCGGCAGTGTTGGTATTGATGACTTCCTGAACGTTAACCTTGCTGCAACCAGTCCGAAT
GCAACCAGTATTGCTTTCCAAGTTGCGAAATTAGGGTCTGATACTTTGTTGGATTTGGAGTTGTCTGCATTGCAACAAGA
ATCAAAAGCAGAAATCATTTCTAGTCCGCGTTTAATTACTACCAATAAGAAGCCGGCTTATATTGAACAAGGTACTGAAA
TTCCTTACCTAGAATCCTCTTCCAGCGGTGCGACATCCGTAACGTTCAAAAAAGCGGTGCTGAGCTTAAAAGTGACACCG
CAGATCACGCCTGATAACCGTTTGGTGCTGGATTTGAGTGTTACGCAAGATAGACCGGGTCAAGTGGTAAAAACTGGTAC
CGGTGAGGCAGTTGCGATTGATACTCAGCGTATTGGCACTCAAGTATTGGTGAATAATGGTGAGACGGTTGTGCTTGGTG
GTATTTTCCAACACAGCATTACAAACTCCGTCGATAAGGTACCTTTATTAGGTGATTTACCGCTGTTAGGGGCACTTTTC
CGTCGAAGTTACGAAAATGTCGGTAAAAGTGAGTTGCTGATTTTTGTGACGCCTAAAGTGGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(423-581)

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

97.084

100

0.971
  pilQ Vibrio cholerae strain A1552

77.329

97.599

0.755
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

77.329

97.599

0.755
  pilQ Pseudomonas aeruginosa PAK

40.138

99.142

0.398
  pilQ Legionella pneumophila strain ERS1305867

41.273

94.34

0.389
  pilQ Legionella pneumophila strain Lp02

41.273

94.34

0.389
  pilQ Acinetobacter baumannii D1279779

36.833

100

0.379
  pilQ Acinetobacter baumannii strain A118

36.833

100

0.379
  comQ Acinetobacter baylyi ADP1

36.986

100

0.37

Multiple sequence alignment