Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CU052_RS07515 Genome accession   NZ_CP025537
Coordinates   1569263..1570279 (+) Length   338 a.a.
NCBI ID   WP_026138410.1    Uniprot ID   -
Organism   Vibrio harveyi strain 345     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1564263..1575279
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CU052_RS07500 (CU052_07900) - 1564636..1565364 (-) 729 WP_005451494.1 redoxin family protein -
  CU052_RS07505 (CU052_07905) oxyR 1565539..1566447 (+) 909 WP_101904441.1 DNA-binding transcriptional regulator OxyR -
  CU052_RS07510 (CU052_07910) - 1566514..1569084 (-) 2571 WP_050902270.1 penicillin-binding protein 1A -
  CU052_RS07515 (CU052_07915) pilM 1569263..1570279 (+) 1017 WP_026138410.1 type IV pilus assembly protein PilM Machinery gene
  CU052_RS07520 (CU052_07920) pilN 1570263..1570838 (+) 576 WP_005451499.1 PilN domain-containing protein Machinery gene
  CU052_RS07525 (CU052_07925) pilO 1570831..1571424 (+) 594 WP_005451500.1 type 4a pilus biogenesis protein PilO Machinery gene
  CU052_RS07530 (CU052_07930) pilP 1571414..1571929 (+) 516 WP_005451501.1 pilus assembly protein PilP Machinery gene
  CU052_RS07535 (CU052_07935) pilQ 1571964..1573715 (+) 1752 WP_005451505.1 type IV pilus secretin PilQ Machinery gene
  CU052_RS07540 (CU052_07940) aroK 1573905..1574423 (+) 519 WP_005451507.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38053.43 Da        Isoelectric Point: 6.4206

>NTDB_id=262393 CU052_RS07515 WP_026138410.1 1569263..1570279(+) (pilM) [Vibrio harveyi strain 345]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEIRKELPFWARRVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFVKLDADEQMRGTDSYQVFATRKDVVETRVDALQKSGLKTV
LVDVHSQSLGHIWKLAVERFPEKSKYCLLDISALSSSFTMFTEQSELFHKEFPYGTRTSNDLFQQDPLAEDTQVATEQFN
RQIVDRVKRQIQLYTSINGSQDIKGIWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGKHAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=262393 CU052_RS07515 WP_026138410.1 1569263..1570279(+) (pilM) [Vibrio harveyi strain 345]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCCTTATTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATAAGAAAAGAGCTGCCATTTTGGGCTCGACGGGTCGCAATTTCTGTTCCTGACAACGCT
GTGATCAGCAAGAAGCTGCAAATTGAGCAGAACTTGGAGGAGAGTGAGAAAGAGTTTGCCGTGGTGCAAGCTTTCTCGCA
TCAATCCCCATTTCCTGTTGAAGAGCTCAGTCTTGATTTTGTAAAGCTTGATGCCGATGAGCAGATGCGTGGAACAGACA
GTTATCAAGTATTCGCGACTCGCAAAGATGTCGTTGAAACTCGTGTCGATGCTCTGCAAAAGTCAGGCTTGAAAACCGTG
CTGGTGGACGTGCATTCACAAAGTTTAGGGCATATATGGAAACTGGCTGTCGAGCGCTTCCCTGAAAAGAGCAAGTATTG
CTTGTTGGATATCAGTGCATTGAGCAGCTCTTTCACCATGTTTACAGAGCAGAGTGAATTGTTCCACAAGGAATTTCCTT
ACGGCACGCGAACTTCAAATGATCTATTTCAGCAAGACCCTCTTGCTGAAGACACGCAAGTTGCTACTGAGCAGTTTAAT
CGACAAATCGTTGATCGTGTAAAGCGCCAGATTCAGCTCTATACCTCGATTAATGGTAGTCAAGACATCAAAGGCATTTG
GTTGTCTGGTGAAGGTGCAGCTACGCCAATGTTAGCAGAAGAACTTTCAAGTCAGTTGGCATTGGAGTGTGAGTTGCTTA
ATCCGCTCGGTCTATTCGAGATGAAAGTATCGAAACGCAAACGCCGTGCAGCAGATTGGCAGCATTTTACTGCGGCTGCG
GGCTTAGCGGTTAGAGGTATTCAATGGCTAGGAGGCAAACATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

90.828

100

0.908
  pilM Vibrio cholerae strain A1552

48.83

100

0.494

Multiple sequence alignment