Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB   Type   Machinery gene
Locus tag   LLG50_RS02010 Genome accession   NZ_CP025500
Coordinates   402524..402913 (+) Length   129 a.a.
NCBI ID   WP_010905314.1    Uniprot ID   S6EQX8
Organism   Lactococcus lactis subsp. lactis strain G50     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 367658..424689 402524..402913 within 0


Gene organization within MGE regions


Location: 367658..424689
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LLG50_RS01840 (LLG50_01840) - 367724..368059 (+) 336 WP_012898553.1 hypothetical protein -
  LLG50_RS01845 (LLG50_01845) - 368062..368568 (+) 507 WP_012898552.1 DUF961 family protein -
  LLG50_RS01850 (LLG50_01850) - 368578..370197 (+) 1620 WP_103054445.1 FtsK/SpoIIIE domain-containing protein -
  LLG50_RS01855 (LLG50_01855) - 370577..371854 (+) 1278 WP_103054446.1 replication initiation factor domain-containing protein -
  LLG50_RS01860 (LLG50_01860) - 371879..372142 (+) 264 WP_103054447.1 hypothetical protein -
  LLG50_RS01865 (LLG50_01865) - 372179..372439 (+) 261 WP_103054448.1 hypothetical protein -
  LLG50_RS01870 (LLG50_01870) - 372553..372813 (+) 261 WP_103054449.1 histidinol-phosphate/aromatic aminotransferase and cobyric acid decarboxylase -
  LLG50_RS11780 (LLG50_01875) - 372901..373326 (+) 426 WP_272938650.1 helix-turn-helix domain-containing protein -
  LLG50_RS01880 (LLG50_01880) - 373415..373840 (+) 426 WP_103054450.1 hypothetical protein -
  LLG50_RS01885 (LLG50_01885) - 374155..376998 (+) 2844 WP_161494983.1 SpaA isopeptide-forming pilin-related protein -
  LLG50_RS01890 (LLG50_01890) - 377123..377323 (+) 201 WP_058225862.1 hypothetical protein -
  LLG50_RS01895 (LLG50_01895) - 377327..378595 (+) 1269 WP_012898547.1 conjugal transfer protein -
  LLG50_RS01900 (LLG50_01900) - 378601..378834 (+) 234 WP_012898546.1 TcpD family membrane protein -
  LLG50_RS01905 (LLG50_01905) - 378843..379208 (+) 366 WP_017865115.1 TcpE family conjugal transfer membrane protein -
  LLG50_RS01910 (LLG50_01910) - 379208..381775 (+) 2568 WP_103054451.1 ATP-binding protein -
  LLG50_RS01915 (LLG50_01915) - 381776..383821 (+) 2046 WP_103054452.1 MnhB domain-containing protein -
  LLG50_RS01920 (LLG50_01920) - 383836..384900 (+) 1065 WP_103054453.1 phage tail tip lysozyme -
  LLG50_RS01925 (LLG50_01925) - 384995..385489 (+) 495 WP_103054454.1 hypothetical protein -
  LLG50_RS01930 (LLG50_01930) - 385514..386005 (+) 492 WP_103054455.1 antirestriction protein ArdA -
  LLG50_RS01935 (LLG50_01935) - 386064..386273 (+) 210 WP_103054456.1 DNA-binding protein -
  LLG50_RS01940 (LLG50_01940) - 386325..387485 (+) 1161 WP_103054457.1 tyrosine-type recombinase/integrase -
  LLG50_RS01945 (LLG50_01945) thiI 387645..388865 (+) 1221 WP_103054458.1 tRNA uracil 4-sulfurtransferase ThiI -
  LLG50_RS01950 (LLG50_01950) - 389078..390481 (+) 1404 WP_103054459.1 MFS transporter -
  LLG50_RS01955 (LLG50_01955) - 390643..391248 (+) 606 WP_015425833.1 FMN-dependent NADH-azoreductase -
  LLG50_RS01960 (LLG50_01960) - 391344..391778 (+) 435 WP_039114601.1 OsmC family protein -
  LLG50_RS01965 (LLG50_01965) - 391814..393856 (-) 2043 WP_003131601.1 cation:proton antiporter -
  LLG50_RS01970 (LLG50_01970) - 393947..394417 (-) 471 WP_010905310.1 hypothetical protein -
  LLG50_RS01975 (LLG50_01975) tyrS 394540..395799 (-) 1260 WP_003131599.1 tyrosine--tRNA ligase -
  LLG50_RS01980 (LLG50_01980) - 395944..398349 (+) 2406 WP_103054460.1 transglycosylase domain-containing protein -
  LLG50_RS01985 (LLG50_01985) pepA 398407..399474 (-) 1068 WP_103054461.1 glutamyl aminopeptidase -
  LLG50_RS01990 (LLG50_01990) - 399577..399861 (+) 285 WP_012897223.1 hypothetical protein -
  LLG50_RS01995 (LLG50_01995) - 399903..400220 (+) 318 WP_003131595.1 thioredoxin family protein -
  LLG50_RS02000 (LLG50_02000) ytpR 400306..400932 (+) 627 WP_003131594.1 YtpR family tRNA-binding protein -
  LLG50_RS02005 (LLG50_02005) nox 401095..402435 (+) 1341 WP_058219163.1 H2O-forming NADH oxidase -
  LLG50_RS02010 (LLG50_02010) ssbB 402524..402913 (+) 390 WP_010905314.1 single-stranded DNA-binding protein Machinery gene
  LLG50_RS02015 (LLG50_02015) groES 403033..403317 (+) 285 WP_003131589.1 co-chaperone GroES -
  LLG50_RS02020 (LLG50_02020) groL 403405..405033 (+) 1629 WP_058206244.1 chaperonin GroEL -
  LLG50_RS02025 (LLG50_02025) - 405078..405890 (-) 813 WP_003131583.1 MBL fold metallo-hydrolase -
  LLG50_RS02030 (LLG50_02030) - 406061..407503 (-) 1443 WP_010905315.1 sensor histidine kinase -
  LLG50_RS02035 (LLG50_02035) yycF 407496..408197 (-) 702 WP_003131580.1 response regulator YycF -
  LLG50_RS02040 (LLG50_02040) tmk 408375..409010 (+) 636 WP_103054462.1 dTMP kinase -
  LLG50_RS02045 (LLG50_02045) - 409143..410003 (+) 861 WP_057719679.1 DNA polymerase III subunit delta' -
  LLG50_RS02050 (LLG50_02050) ricT 410055..410837 (+) 783 WP_010905316.1 PSP1 domain-containing protein -
  LLG50_RS02055 (LLG50_02055) yabA 410830..411156 (+) 327 WP_003131570.1 DNA replication initiation control protein YabA -
  LLG50_RS02060 (LLG50_02060) rsmI 411156..412031 (+) 876 WP_103054463.1 16S rRNA (cytidine(1402)-2'-O)-methyltransferase -
  LLG50_RS02065 (LLG50_02065) - 412108..412299 (-) 192 WP_003131566.1 hypothetical protein -
  LLG50_RS02070 (LLG50_02070) mvk 412362..413294 (-) 933 WP_003131564.1 mevalonate kinase -
  LLG50_RS02075 (LLG50_02075) mvaD 413600..414556 (+) 957 WP_058219167.1 diphosphomevalonate decarboxylase -
  LLG50_RS02080 (LLG50_02080) - 414543..415532 (+) 990 WP_021721904.1 mevalonate kinase -
  LLG50_RS02085 (LLG50_02085) fni 415587..416630 (+) 1044 WP_103054464.1 type 2 isopentenyl-diphosphate Delta-isomerase -
  LLG50_RS02090 (LLG50_02090) - 416788..417408 (+) 621 WP_003131560.1 superoxide dismutase -
  LLG50_RS02095 (LLG50_02095) - 417656..420010 (+) 2355 WP_103054465.1 carbon starvation CstA family protein -
  LLG50_RS02100 (LLG50_02100) - 420312..421652 (+) 1341 WP_017864136.1 DEAD/DEAH box helicase -
  LLG50_RS02105 (LLG50_02105) - 421656..422240 (+) 585 WP_095586708.1 YdcF family protein -
  LLG50_RS02110 (LLG50_02110) - 422396..423142 (+) 747 WP_010905323.1 MurR/RpiR family transcriptional regulator -
  LLG50_RS02115 (LLG50_02115) - 423384..423710 (+) 327 WP_003131553.1 PTS sugar transporter subunit IIB -
  LLG50_RS02120 (LLG50_02120) - 423795..424145 (+) 351 WP_003131552.1 PTS lactose/cellobiose transporter subunit IIA -

Sequence


Protein


Download         Length: 129 a.a.        Molecular weight: 14672.73 Da        Isoelectric Point: 8.3705

>NTDB_id=262083 LLG50_RS02010 WP_010905314.1 402524..402913(+) (ssbB) [Lactococcus lactis subsp. lactis strain G50]
MNKTMLIGRLTSAPEISKTTNDKSYVRVTLAVNRRFKNEKGEREADFISIIIWGKSAETLVSYAKKGSLISIEGEIRTRN
YTDKQNQKHYVTEILGLSYDLLESRATIALRESAIKTEETLLDAEELPF

Nucleotide


Download         Length: 390 bp        

>NTDB_id=262083 LLG50_RS02010 WP_010905314.1 402524..402913(+) (ssbB) [Lactococcus lactis subsp. lactis strain G50]
ATGAATAAAACCATGTTAATTGGACGCTTAACCAGTGCGCCAGAAATTTCAAAAACAACAAATGATAAATCATATGTCCG
TGTGACCTTAGCAGTCAATCGCCGTTTCAAAAATGAAAAAGGAGAACGAGAGGCAGATTTTATTTCAATTATTATTTGGG
GAAAATCTGCAGAAACTTTAGTTTCTTACGCAAAAAAAGGAAGTCTTATTTCAATTGAAGGAGAAATTAGAACTAGAAAT
TATACGGATAAGCAAAATCAAAAACATTATGTCACAGAAATCTTAGGACTGAGTTATGATTTACTTGAAAGTAGGGCAAC
AATCGCTTTACGAGAAAGCGCTATAAAGACTGAAGAAACCTTACTTGATGCGGAAGAACTCCCTTTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB S6EQX8

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB Lactococcus lactis subsp. cremoris KW2

90.698

100

0.907

  ssbB Streptococcus sobrinus strain NIDR 6715-7

60.769

100

0.612

  ssbB/cilA Streptococcus pneumoniae TIGR4

56.923

100

0.574

  ssbB/cilA Streptococcus pneumoniae Rx1

56.154

100

0.566

  ssbB/cilA Streptococcus pneumoniae D39

56.154

100

0.566

  ssbB/cilA Streptococcus pneumoniae R6

56.154

100

0.566

  ssbB/cilA Streptococcus mitis NCTC 12261

56.154

100

0.566

  ssbB/cilA Streptococcus mitis SK321

56.154

100

0.566

  ssbA Streptococcus mutans UA159

55.385

100

0.558

  ssb Latilactobacillus sakei subsp. sakei 23K

43.75

86.822

0.38

  ssbA Bacillus subtilis subsp. subtilis str. 168

44.762

81.395

0.364


Multiple sequence alignment