Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbB/cilA   Type   Machinery gene
Locus tag   CWI64_RS05040 Genome accession   NZ_CP025076
Coordinates   943246..943641 (-) Length   131 a.a.
NCBI ID   WP_000282467.1    Uniprot ID   A0A098Z0L0
Organism   Streptococcus pneumoniae strain BHN97x     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 938678..953754 943246..943641 within 0


Gene organization within MGE regions


Location: 938678..953754
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CWI64_RS11650 - 938678..939043 (-) 366 WP_000800711.1 helix-turn-helix transcriptional regulator -
  CWI64_RS05020 (CWI64_05020) - 939056..939829 (-) 774 WP_044815107.1 class I SAM-dependent methyltransferase -
  CWI64_RS05025 (CWI64_05025) - 940275..941030 (-) 756 Protein_933 epoxyqueuosine reductase QueH -
  CWI64_RS05030 (CWI64_05030) groL 941168..942790 (-) 1623 WP_000031577.1 chaperonin GroEL -
  CWI64_RS05035 (CWI64_05035) groES 942806..943090 (-) 285 WP_000917304.1 co-chaperone GroES -
  CWI64_RS05040 (CWI64_05040) ssbB/cilA 943246..943641 (-) 396 WP_000282467.1 single-stranded DNA-binding protein Machinery gene
  CWI64_RS05045 (CWI64_05045) - 943719..944480 (-) 762 WP_001107755.1 SDR family NAD(P)-dependent oxidoreductase -
  CWI64_RS05050 (CWI64_05050) ytpR 944513..945139 (-) 627 WP_000578309.1 YtpR family tRNA-binding protein -
  CWI64_RS05055 (CWI64_05055) - 945155..945472 (-) 318 WP_000615767.1 thioredoxin family protein -
  CWI64_RS05060 (CWI64_05060) - 945469..945768 (-) 300 WP_001013974.1 DUF4651 domain-containing protein -
  CWI64_RS12280 - 945862..945993 (+) 132 WP_001811306.1 hypothetical protein -
  CWI64_RS11970 - 946018..946080 (-) 63 WP_219300018.1 hypothetical protein -
  CWI64_RS05065 (CWI64_05065) - 946105..946296 (-) 192 WP_000291826.1 cell wall-binding protein -
  CWI64_RS05070 (CWI64_05070) - 946457..947086 (-) 630 WP_239424734.1 hypothetical protein -
  CWI64_RS05080 (CWI64_05080) - 947142..948314 (+) 1173 WP_100931674.1 IS256-like element IS256 family transposase -
  CWI64_RS05085 (CWI64_05085) aph(3')-IIIa 948419..949213 (-) 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
  CWI64_RS05090 (CWI64_05090) - 949234..950346 (-) 1113 WP_033875254.1 long-chain-fatty-acid--CoA ligase -
  CWI64_RS05095 (CWI64_05095) - 950366..951289 (-) 924 WP_049581805.1 acyl transferase -
  CWI64_RS05100 (CWI64_05100) - 951309..952751 (-) 1443 WP_100931675.1 acyl-CoA reductase -
  CWI64_RS05105 (CWI64_05105) - 952771..953754 (-) 984 WP_033875255.1 alkanal monooxygenase -

Sequence


Protein


Download         Length: 131 a.a.        Molecular weight: 14925.89 Da        Isoelectric Point: 5.9409

>NTDB_id=258079 CWI64_RS05040 WP_000282467.1 943246..943641(-) (ssbB/cilA) [Streptococcus pneumoniae strain BHN97x]
MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREADFVNMVLWGRLAETLASYATKGSLISVDGELRTR
RFEKNGQMNYVTEVLVTGFQLLESRAQRAMRENNAGQDLADLVLEEEELPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=258079 CWI64_RS05040 WP_000282467.1 943246..943641(-) (ssbB/cilA) [Streptococcus pneumoniae strain BHN97x]
ATGTATAATAAAGTTATCATGATTGGGCGTTTAACGTCTACACCAGAATTGCACAAAACCAACAATGACAAGTCGGTAGC
GCGAGCAACTATCGCTGTCAACCGTCGTTACAAAGACCAAAACGGTGAACGTGAAGCTGATTTTGTCAATATGGTCCTAT
GGGGCAGACTAGCAGAAACTTTGGCAAGCTACGCAACCAAAGGTAGTCTCATTTCCGTTGATGGAGAATTGCGTACCCGT
CGCTTTGAGAAAAATGGTCAGATGAATTATGTAACCGAAGTCCTTGTGACAGGATTCCAACTCTTGGAAAGTCGTGCCCA
ACGTGCTATGCGTGAAAATAATGCAGGCCAAGATTTGGCAGATTTGGTCTTGGAAGAGGAAGAATTGCCATTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A098Z0L0

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbB/cilA Streptococcus pneumoniae D39

100

100

1

  ssbB/cilA Streptococcus pneumoniae Rx1

100

100

1

  ssbB/cilA Streptococcus pneumoniae R6

100

100

1

  ssbB/cilA Streptococcus pneumoniae TIGR4

99.237

100

0.992

  ssbB/cilA Streptococcus mitis SK321

98.473

100

0.985

  ssbB/cilA Streptococcus mitis NCTC 12261

98.473

100

0.985

  ssbA Streptococcus mutans UA159

74.809

100

0.748

  ssbB Streptococcus sobrinus strain NIDR 6715-7

70.229

100

0.702

  ssbB Lactococcus lactis subsp. cremoris KW2

59.821

85.496

0.511

  ssbA Bacillus subtilis subsp. subtilis str. 168

50.943

80.916

0.412

  ssb Latilactobacillus sakei subsp. sakei 23K

46.903

86.26

0.405


Multiple sequence alignment