Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   MFLA_RS10960 Genome accession   NC_007947
Coordinates   2290120..2291328 (+) Length   402 a.a.
NCBI ID   WP_011480363.1    Uniprot ID   Q1GZC8
Organism   Methylobacillus flagellatus KT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2285120..2296328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFLA_RS10940 (Mfla_2138) - 2285954..2286484 (-) 531 WP_011480359.1 rRNA large subunit pseudouridine synthase E -
  MFLA_RS10945 (Mfla_2139) icd 2286543..2287784 (+) 1242 WP_011480360.1 NADP-dependent isocitrate dehydrogenase -
  MFLA_RS10950 (Mfla_2140) - 2287889..2288092 (-) 204 WP_011480361.1 cold-shock protein -
  MFLA_RS10955 (Mfla_2141) pilB 2288393..2290102 (+) 1710 WP_011480362.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MFLA_RS10960 (Mfla_2142) pilC 2290120..2291328 (+) 1209 WP_011480363.1 type II secretion system F family protein Machinery gene
  MFLA_RS10965 (Mfla_2143) - 2291418..2292143 (-) 726 WP_011480364.1 DsbC family protein -
  MFLA_RS10970 (Mfla_2144) - 2292174..2293358 (-) 1185 WP_011480365.1 FAD-dependent monooxygenase -
  MFLA_RS10975 (Mfla_2145) - 2293395..2294567 (-) 1173 WP_048811970.1 FAD-dependent monooxygenase -
  MFLA_RS10980 (Mfla_2146) - 2294570..2295889 (-) 1320 WP_011480367.1 aminopeptidase P N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43901.60 Da        Isoelectric Point: 9.5786

>NTDB_id=25663 MFLA_RS10960 WP_011480363.1 2290120..2291328(+) (pilC) [Methylobacillus flagellatus KT]
MAAKETKEVTYAWEGTDRKGKRVKGEMKASGEAFVKATLRRQGINVVKISKQRFKSGGKITDKDVTLFTRQLATMMKAGV
PLLQAFDIVGKGHSNPAVSKLLGDIKADVETGSSLSQAFRKYPLYFDNLFCNLVGAGEQAGILDSLLDRLATYKEKILAI
KGKIKSALFYPISIIVVAFVITAVIMIFVIPAFKELFSSFGADLPAPTVMVMAISDFFVDYWWAIFGSIGGALWFFFYTW
KRSEKMQSSMDRLLLKMPIFGEVIRKATIARFSRTLSTMFAAGVPLVEALDSVAGASGNRVYHDATKKVQSEISTGASLT
VAMQNTEVFPNMVLQMVAIGEESGALDSMLGKVADFYEGEVDDAVEALSSLMEPIIMVVLGTLIGGLVIAMYLPIFKMGQ
VV

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=25663 MFLA_RS10960 WP_011480363.1 2290120..2291328(+) (pilC) [Methylobacillus flagellatus KT]
ATGGCTGCCAAGGAAACAAAGGAAGTGACGTACGCCTGGGAGGGCACGGACAGGAAAGGCAAGCGCGTCAAGGGGGAGAT
GAAGGCCAGCGGTGAAGCCTTCGTCAAGGCCACGCTGCGCCGCCAAGGTATCAATGTCGTCAAAATCAGCAAACAGCGCT
TCAAGTCCGGCGGCAAGATTACAGACAAGGATGTCACGTTATTCACCCGCCAGCTCGCCACCATGATGAAAGCCGGTGTT
CCTCTGCTACAGGCGTTTGATATCGTCGGGAAGGGGCATAGCAATCCTGCAGTAAGCAAGCTGCTTGGCGACATCAAGGC
TGACGTGGAAACCGGGAGCAGCCTGAGTCAGGCTTTCCGCAAATACCCCTTGTATTTCGACAACCTGTTCTGCAACCTTG
TTGGCGCCGGCGAGCAGGCCGGTATCCTGGACTCCCTGCTGGACAGACTTGCCACCTACAAGGAAAAGATCCTCGCCATC
AAGGGCAAGATCAAGTCGGCCCTGTTCTATCCCATCTCCATTATCGTGGTGGCTTTCGTGATCACCGCCGTCATCATGAT
TTTCGTGATTCCGGCATTCAAGGAGCTGTTCAGCAGCTTCGGTGCCGACTTACCTGCCCCCACCGTCATGGTCATGGCGA
TATCCGATTTCTTCGTTGACTACTGGTGGGCCATCTTCGGATCGATTGGCGGTGCCCTCTGGTTCTTCTTCTACACCTGG
AAACGTTCAGAAAAGATGCAATCCAGCATGGACAGGCTGCTGCTCAAGATGCCCATTTTTGGCGAGGTGATCCGCAAGGC
CACGATTGCACGTTTCTCGCGCACGTTGTCCACCATGTTTGCCGCCGGTGTCCCGCTGGTCGAGGCGCTGGATTCAGTTG
CTGGCGCGTCAGGCAACCGCGTCTATCATGACGCAACCAAGAAGGTTCAGAGCGAGATCAGCACAGGTGCCAGCCTCACC
GTCGCGATGCAGAACACGGAAGTCTTTCCCAATATGGTGCTGCAAATGGTCGCTATCGGCGAGGAGTCCGGCGCACTGGA
TTCCATGCTGGGCAAGGTGGCGGATTTTTACGAGGGTGAAGTGGATGACGCAGTTGAGGCCCTTTCGAGCTTGATGGAGC
CTATCATCATGGTAGTGCTCGGCACCCTGATTGGCGGCCTGGTGATCGCCATGTACCTGCCGATATTCAAGATGGGCCAA
GTAGTGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1GZC8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Acinetobacter baumannii D1279779

53.902

100

0.55
  pilC Pseudomonas stutzeri DSM 10701

54.321

100

0.547
  pilC Legionella pneumophila strain ERS1305867

55.025

99.005

0.545
  pilC Acinetobacter baylyi ADP1

53.676

100

0.545
  pilG Neisseria gonorrhoeae MS11

52.897

98.756

0.522
  pilG Neisseria meningitidis 44/76-A

52.645

98.756

0.52
  pilC Vibrio cholerae strain A1552

42.569

98.756

0.42
  pilC Vibrio campbellii strain DS40M4

40.399

99.751

0.403
  pilC Thermus thermophilus HB27

37.845

99.254

0.376

Multiple sequence alignment