Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MFLA_RS10955 Genome accession   NC_007947
Coordinates   2288393..2290102 (+) Length   569 a.a.
NCBI ID   WP_011480362.1    Uniprot ID   Q1GZC9
Organism   Methylobacillus flagellatus KT     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2283393..2295102
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFLA_RS10930 (Mfla_2136) - 2283571..2284722 (-) 1152 WP_011480357.1 MFS transporter -
  MFLA_RS10935 (Mfla_2137) aroC 2284795..2285880 (-) 1086 WP_011480358.1 chorismate synthase -
  MFLA_RS10940 (Mfla_2138) - 2285954..2286484 (-) 531 WP_011480359.1 rRNA large subunit pseudouridine synthase E -
  MFLA_RS10945 (Mfla_2139) icd 2286543..2287784 (+) 1242 WP_011480360.1 NADP-dependent isocitrate dehydrogenase -
  MFLA_RS10950 (Mfla_2140) - 2287889..2288092 (-) 204 WP_011480361.1 cold-shock protein -
  MFLA_RS10955 (Mfla_2141) pilB 2288393..2290102 (+) 1710 WP_011480362.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MFLA_RS10960 (Mfla_2142) pilC 2290120..2291328 (+) 1209 WP_011480363.1 type II secretion system F family protein Machinery gene
  MFLA_RS10965 (Mfla_2143) - 2291418..2292143 (-) 726 WP_011480364.1 DsbC family protein -
  MFLA_RS10970 (Mfla_2144) - 2292174..2293358 (-) 1185 WP_011480365.1 FAD-dependent monooxygenase -
  MFLA_RS10975 (Mfla_2145) - 2293395..2294567 (-) 1173 WP_048811970.1 FAD-dependent monooxygenase -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62703.00 Da        Isoelectric Point: 4.8207

>NTDB_id=25662 MFLA_RS10955 WP_011480362.1 2288393..2290102(+) (pilB) [Methylobacillus flagellatus KT]
MAATVSNKLGGLARVLIHQNMLSEDEAVTIQAAASDKQISFISQLLQSRKFTALQIAQASSQAFGYPLLDLNTLNPEFLP
SKGISPQLMQSYRVITLYNRGNNMFVALSDPTNLPMLDEIQFQTGKTLLPVIVEDDKLGKWIDRLVEASDTTMQSLVSED
DFGLESEEKESEPEPTLEIEDAPVVKYIQKILLDAINMGASDLHFEPYEKFYRIRYRVDGILREIAQPPLAIKDKLASRI
KVISNLDISEKRIPQDGRMKLVLSKTRTIDFRVSTLPLINGEKIVMRILDPSSAKLGIDALGYEPDQKEALLHAVTRPYG
LVLVTGPTGSGKTVSLYTCLNILNEPGVNISTAEDPAEIPLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMIGEIRDL
ETADMAIKAASTGHMVLSTLHTNDAPSTLTRLVNMGVAPFNIASAVSLITAQRLARKLCPHCKVPADIPRETLLDIGFKE
EDLDGSWQPYAANPEGCEYCTLGYKGRVGIYQVMPVSEEMSRIIMKNGTSHDIADQAQREGIRDLRQSGLLKVKQGLTSL
EEIEAVTNE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=25662 MFLA_RS10955 WP_011480362.1 2288393..2290102(+) (pilB) [Methylobacillus flagellatus KT]
ATGGCTGCCACCGTATCGAACAAACTGGGCGGACTCGCTCGTGTATTGATTCACCAGAACATGCTGAGCGAGGATGAGGC
CGTCACCATCCAGGCAGCTGCCAGCGACAAGCAGATTTCATTCATCAGCCAGCTGCTGCAATCGCGCAAGTTCACTGCAT
TGCAAATCGCCCAGGCATCCTCCCAGGCCTTTGGTTATCCATTGCTGGACCTCAATACGTTAAACCCCGAATTCCTTCCC
AGCAAAGGCATCAGCCCGCAACTCATGCAGAGCTACCGCGTCATCACGCTCTACAATCGCGGCAACAATATGTTTGTGGC
CTTGTCCGACCCCACCAACCTACCCATGCTTGATGAAATCCAGTTCCAGACCGGCAAGACATTGTTGCCGGTCATTGTCG
AGGATGACAAGCTGGGGAAATGGATAGACAGACTGGTGGAAGCAAGTGACACCACCATGCAATCCCTGGTCAGTGAAGAC
GACTTCGGCCTGGAGTCGGAAGAAAAGGAGAGCGAGCCGGAGCCCACGCTCGAAATTGAAGACGCCCCAGTCGTCAAGTA
CATCCAGAAAATCTTGCTCGACGCGATCAACATGGGCGCATCCGACCTGCATTTCGAACCCTATGAGAAGTTTTACCGCA
TCCGCTACCGTGTAGACGGCATCCTGCGCGAGATTGCCCAGCCGCCGCTTGCGATCAAGGATAAGCTGGCATCACGCATC
AAGGTCATCTCCAACCTCGACATCTCGGAAAAACGCATTCCCCAGGATGGCCGTATGAAACTGGTGCTGTCCAAGACACG
CACCATCGATTTTCGTGTCAGCACGCTGCCCCTGATCAACGGCGAGAAAATCGTGATGCGTATCCTCGACCCCAGCAGCG
CCAAGCTCGGGATCGACGCCCTTGGCTATGAGCCCGACCAGAAGGAGGCATTGCTCCATGCAGTGACCCGCCCTTATGGC
CTGGTCCTGGTCACGGGTCCGACCGGGTCAGGAAAAACGGTTTCACTGTATACCTGCCTCAATATCCTCAACGAGCCCGG
CGTGAACATTTCCACGGCGGAAGACCCTGCGGAAATCCCGTTGCCCGGCATCAACCAGGTCAATGTCAATGACAAACAAG
GGCTGACATTCGCAGCGGCCCTGAAATCCTTCCTGCGGCAGGATCCGGACATCATCATGATCGGGGAAATTCGTGATCTG
GAAACTGCCGACATGGCGATCAAGGCAGCATCTACCGGCCATATGGTGCTTTCCACCCTGCATACCAATGATGCCCCCTC
CACCTTGACGCGACTGGTGAATATGGGGGTGGCCCCCTTCAATATTGCCTCTGCCGTGTCGTTAATAACAGCACAGCGCC
TGGCGCGCAAGCTGTGCCCGCATTGCAAGGTACCGGCCGACATTCCGCGCGAAACCCTGCTAGACATTGGTTTCAAGGAG
GAGGATCTGGACGGCAGCTGGCAGCCCTATGCAGCCAACCCTGAAGGATGCGAGTACTGTACCCTTGGCTACAAGGGCCG
GGTCGGGATTTACCAGGTCATGCCGGTATCCGAGGAGATGAGCCGCATCATCATGAAAAACGGCACATCGCACGACATTG
CAGACCAGGCACAGCGGGAAGGCATCCGCGACTTGCGGCAATCCGGCTTGCTCAAGGTCAAGCAGGGGCTGACCTCGCTG
GAGGAAATTGAAGCTGTCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1GZC9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.356

100

0.545
  pilB Acinetobacter baumannii D1279779

53.603

100

0.536
  pilF Neisseria gonorrhoeae MS11

53.996

98.946

0.534
  pilB Legionella pneumophila strain ERS1305867

52.273

100

0.525
  pilB Vibrio campbellii strain DS40M4

47.257

99.297

0.469
  pilB Vibrio cholerae strain A1552

47.5

98.418

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

46.996

99.473

0.467
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.279

92.619

0.364
  pilF Thermus thermophilus HB27

38.619

94.2

0.364

Multiple sequence alignment