Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   FORC73_RS02495 Genome accession   NZ_CP024082
Coordinates   505167..505622 (-) Length   151 a.a.
NCBI ID   WP_162923374.1    Uniprot ID   -
Organism   Vibrio cholerae strain FORC_073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 500167..510622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC73_RS02475 (FORC73_0451) coaE 500672..501280 (-) 609 WP_000011549.1 dephospho-CoA kinase -
  FORC73_RS02480 (FORC73_0452) pilD 501277..502152 (-) 876 WP_102136994.1 A24 family peptidase Machinery gene
  FORC73_RS02485 (FORC73_0453) pilC 502210..503436 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  FORC73_RS02490 (FORC73_0454) pilB 503479..505167 (-) 1689 WP_119783037.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC73_RS02495 (FORC73_0455) pilA 505167..505622 (-) 456 WP_162923374.1 pilin Machinery gene
  FORC73_RS02500 (FORC73_0456) nadC 505867..506757 (-) 891 WP_032470410.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC73_RS02510 (FORC73_0457) ampD 507059..507604 (+) 546 WP_119783039.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC73_RS02515 (FORC73_0458) fldB 507755..508273 (-) 519 WP_000690112.1 flavodoxin FldB -
  FORC73_RS02520 (FORC73_0459) xerD 508411..509319 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  FORC73_RS02525 (FORC73_0460) dsbC 509336..510088 (+) 753 WP_000115052.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 151 a.a.        Molecular weight: 15569.56 Da        Isoelectric Point: 8.4553

>NTDB_id=251724 FORC73_RS02495 WP_162923374.1 505167..505622(-) (pilA) [Vibrio cholerae strain FORC_073]
MNAYKNKLQQGFTLIELMIVVAVIGVLAAIAVPQYQKYVAKSEAASALATLTGLKTNAEAYTVETGSFPTDTQQAQLGTP
SSAMGSIAYANSGNSGAAGTITFSFTGTVSPSLKNSAIRLKRDGSGNWSCESKTSLDSGVIPKGCNSGTFE

Nucleotide


Download         Length: 456 bp        

>NTDB_id=251724 FORC73_RS02495 WP_162923374.1 505167..505622(-) (pilA) [Vibrio cholerae strain FORC_073]
ATGAACGCGTATAAAAACAAACTACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCTGTAATTGGGGTGCT
AGCAGCAATTGCGGTGCCTCAATACCAGAAATACGTAGCGAAAAGTGAAGCAGCTTCTGCATTAGCTACGTTAACTGGTT
TAAAAACCAATGCAGAGGCTTATACCGTTGAAACAGGATCTTTTCCCACAGATACTCAACAAGCACAACTAGGTACACCA
TCATCTGCAATGGGTTCCATCGCATATGCAAATTCAGGAAATAGTGGTGCAGCAGGAACAATAACATTTTCTTTTACTGG
TACAGTTAGCCCTAGTTTGAAAAACAGTGCTATCCGTTTAAAACGAGATGGCTCAGGAAACTGGTCATGTGAATCAAAAA
CTAGTTTAGATAGTGGAGTGATCCCTAAAGGATGTAATTCTGGCACTTTCGAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(40-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

50

100

0.51
  pilA Vibrio cholerae strain A1552

50

100

0.51
  pilA Vibrio cholerae C6706

50

100

0.51
  pilA Pseudomonas aeruginosa PAK

46.358

100

0.464
  pilA Vibrio parahaemolyticus RIMD 2210633

46.212

87.417

0.404
  pilA Acinetobacter baumannii strain A118

39.333

99.338

0.391
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.31

96.026

0.377
  pilA/pilAII Pseudomonas stutzeri DSM 10701

39.161

94.702

0.371

Multiple sequence alignment