Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC73_RS02490 Genome accession   NZ_CP024082
Coordinates   503479..505167 (-) Length   562 a.a.
NCBI ID   WP_119783037.1    Uniprot ID   -
Organism   Vibrio cholerae strain FORC_073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498479..510167
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC73_RS02465 (FORC73_0449) yacG 499545..499742 (-) 198 WP_000162865.1 DNA gyrase inhibitor YacG -
  FORC73_RS02470 (FORC73_0450) zapD 499900..500640 (-) 741 WP_000207194.1 cell division protein ZapD -
  FORC73_RS02475 (FORC73_0451) coaE 500672..501280 (-) 609 WP_000011549.1 dephospho-CoA kinase -
  FORC73_RS02480 (FORC73_0452) pilD 501277..502152 (-) 876 WP_102136994.1 A24 family peptidase Machinery gene
  FORC73_RS02485 (FORC73_0453) pilC 502210..503436 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  FORC73_RS02490 (FORC73_0454) pilB 503479..505167 (-) 1689 WP_119783037.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC73_RS02495 (FORC73_0455) pilA 505167..505622 (-) 456 WP_162923374.1 pilin Machinery gene
  FORC73_RS02500 (FORC73_0456) nadC 505867..506757 (-) 891 WP_032470410.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC73_RS02510 (FORC73_0457) ampD 507059..507604 (+) 546 WP_119783039.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC73_RS02515 (FORC73_0458) fldB 507755..508273 (-) 519 WP_000690112.1 flavodoxin FldB -
  FORC73_RS02520 (FORC73_0459) xerD 508411..509319 (+) 909 WP_001287472.1 site-specific tyrosine recombinase XerD -
  FORC73_RS02525 (FORC73_0460) dsbC 509336..510088 (+) 753 WP_000115052.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62074.23 Da        Isoelectric Point: 6.0856

>NTDB_id=251723 FORC73_RS02490 WP_119783037.1 503479..505167(-) (pilB) [Vibrio cholerae strain FORC_073]
MLTNLIAILRQAELISATQEQAVVTQVSASGTSVPEALLGLGIFHPQELTEQLSHIFGLPETDLSRYDYAPLCQQLGLRE
LITRYDALPIAKQGNLLLLAVSDPTLLQAEEEFRFATGLQVELALADHRALQAAIRRLYGRSIQGAANQGKEISQDELAT
LVKVSDDELQSIEDLSQDDSPVSRFINQVLLDAVRKGASDIHFEPYENQYRIRLRCDGILVETQQPASHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLSRDTAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYNPQQKQLYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRILNTSQINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGVEPFNLASSLSLIIAQRLARRLCKHCKIAVRPSALLQSQFAFQLNE
ILYEANAAGCNECTGGYSGRVGIYEVMAFNTELAEAIMQRASIHQIERLAKANGMQTLQESGLEKLREGITSFAELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=251723 FORC73_RS02490 WP_119783037.1 503479..505167(-) (pilB) [Vibrio cholerae strain FORC_073]
ATGCTCACCAACCTTATTGCTATCTTGCGTCAGGCGGAATTAATCAGCGCAACGCAAGAACAAGCGGTGGTTACACAGGT
TAGCGCTTCGGGGACTTCGGTGCCCGAGGCGCTGCTTGGTTTAGGGATTTTTCACCCCCAAGAGCTGACCGAGCAACTGA
GCCATATTTTCGGCTTGCCGGAAACCGACCTTAGCCGCTACGACTACGCCCCCCTGTGCCAACAGCTTGGGCTGCGTGAA
CTGATTACCCGCTACGATGCCTTGCCGATTGCCAAACAAGGCAATTTATTGCTGCTCGCGGTCTCAGACCCGACCTTACT
GCAAGCCGAAGAAGAGTTTCGTTTTGCGACTGGGCTGCAAGTTGAACTGGCACTGGCCGATCACCGCGCGCTGCAAGCCG
CGATTCGCCGTTTGTATGGCCGCTCGATTCAAGGCGCAGCCAACCAAGGGAAAGAGATCAGCCAAGATGAGCTCGCCACT
CTGGTCAAGGTCAGCGATGACGAACTGCAATCCATTGAAGACTTAAGCCAAGATGACTCACCGGTTAGCCGCTTTATCAA
CCAAGTGCTGCTCGATGCGGTACGTAAAGGTGCCTCGGATATTCATTTTGAGCCGTATGAAAACCAGTATCGGATCCGCC
TGCGCTGCGATGGGATCTTGGTCGAAACTCAGCAACCGGCTAGCCATTTAAGCCGCCGTTTAGCCGCGCGGATTAAAATT
CTCTCCAAATTAGATATTGCTGAGCGCCGTTTGCCGCAAGACGGGCGGATTAAACTGCGCCTAAGCCGCGATACCGCCAT
TGATATGCGTGTATCCACGCTTCCCACTTTATGGGGCGAAAAAATCGTGCTGCGTCTGCTCGATAGCAGCGCCGCCAATC
TGGATATTGATAAGCTCGGCTATAACCCACAGCAAAAGCAGCTCTATCTCAACGCCCTCAAAAGACCGCAAGGGATGATC
TTGATGACTGGCCCCACCGGCAGCGGTAAAACCGTTTCGCTCTATACAGGGTTGCGCATTCTCAACACGTCACAAATCAA
TATCTCCACCGCGGAAGATCCAGTAGAAATTAACCTCTCTGGGATTAACCAAGTGCAAGTGCAGCCGAAAATCGGCTTTG
GCTTTGCCGAAGCGCTACGCTCGTTTCTGCGCCAAGACCCTGATGTAGTGATGGTCGGCGAAATACGCGATCTGGAAACT
GCAGAAATCGCGGTCAAAGCCGCGCAAACCGGCCACTTGGTGCTTTCCACCCTGCACACCAACTCGGCCGCTGAAACCGT
AATTCGTTTAGCCAATATGGGGGTGGAGCCGTTTAACCTTGCGTCATCACTCAGTTTAATCATCGCCCAACGCCTCGCGC
GCCGCCTATGTAAACACTGCAAAATCGCGGTGCGCCCTTCCGCCCTATTGCAAAGCCAATTTGCATTTCAGCTCAATGAA
ATCTTGTATGAAGCGAATGCGGCGGGGTGTAACGAGTGTACGGGCGGCTATTCAGGGCGCGTAGGGATCTATGAAGTGAT
GGCGTTTAATACCGAGCTGGCAGAAGCCATCATGCAGCGCGCCAGCATTCATCAAATTGAACGTTTAGCCAAAGCCAATG
GCATGCAAACGTTGCAAGAGTCCGGCCTTGAAAAGCTGCGCGAAGGCATCACCAGCTTTGCCGAGCTGCAGCGTGTGCTC
TATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

98.754

100

0.988
  pilB Vibrio parahaemolyticus RIMD 2210633

73.843

100

0.738
  pilB Vibrio campbellii strain DS40M4

73.665

100

0.737
  pilB Legionella pneumophila strain ERS1305867

53.033

90.925

0.482
  pilB Acinetobacter baylyi ADP1

50

93.238

0.466
  pilB Acinetobacter baumannii D1279779

50

92.527

0.463
  pilF Neisseria gonorrhoeae MS11

45.907

100

0.459
  pilB/pilB1 Synechocystis sp. PCC 6803

37.086

100

0.399
  pilF Thermus thermophilus HB27

39.372

96.263

0.379
  pilB Haemophilus influenzae 86-028NP

39.469

93.772

0.37

Multiple sequence alignment