Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CK911_RS14805 Genome accession   NZ_CP023817
Coordinates   3324275..3325516 (+) Length   413 a.a.
NCBI ID   WP_068978945.1    Uniprot ID   A0A291TLJ7
Organism   Aeromonas sp. CU5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3319275..3330516
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK911_RS14775 (CK911_14775) ampD 3319503..3320087 (-) 585 WP_098970877.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CK911_RS14780 (CK911_14780) - 3320218..3320688 (+) 471 WP_098970879.1 TIGR02281 family clan AA aspartic protease -
  CK911_RS14785 (CK911_14785) nadC 3320692..3321567 (+) 876 WP_098970881.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CK911_RS14795 (CK911_14795) - 3321894..3322328 (+) 435 WP_098972701.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CK911_RS14800 (CK911_14800) pilB 3322328..3324034 (+) 1707 WP_098970883.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  CK911_RS14805 (CK911_14805) pilC 3324275..3325516 (+) 1242 WP_068978945.1 type II secretion system F family protein Machinery gene
  CK911_RS14810 (CK911_14810) pilD 3325620..3326492 (+) 873 WP_098970886.1 A24 family peptidase Machinery gene
  CK911_RS14815 (CK911_14815) coaE 3326496..3327110 (+) 615 WP_098970888.1 dephospho-CoA kinase -
  CK911_RS14820 (CK911_14820) zapD 3327148..3327870 (+) 723 WP_068978942.1 cell division protein ZapD -
  CK911_RS14825 (CK911_14825) yacG 3327883..3328077 (+) 195 WP_042019568.1 DNA gyrase inhibitor YacG -
  CK911_RS14830 (CK911_14830) mutT 3328149..3328553 (-) 405 WP_098970892.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 413 a.a.        Molecular weight: 45223.32 Da        Isoelectric Point: 9.7631

>NTDB_id=250308 CK911_RS14805 WP_068978945.1 3324275..3325516(+) (pilC) [Aeromonas sp. CU5]
MATLAKKSNAPKKVFAFRWHGVNRKGQKVSGELQADSISTVKAELRKQGVNVTKVAKKSQGLFSKGGAKIKPMDIAIVSR
QITTMLSAGVPLVQSLQIIARSHEKAAMRELMGQIASDVETGTPMSEALRRHPRHFDELYCDLVEAGEQSGALETIYDRI
ATYREKSEALKSKIKKAMFYPAMVMLVAIVVTSILLLFVIPQFEDIFKSFGAELPAFTQFVIGISRFMQNWWYLIFGGVG
LGIFLYVRAWRASQTVKDNTDKFVLTIPVVGMILHKAAMARFARTLSTTFSAGIPLVDALISAAGASGNYVYRTAVLAIR
NEVVAGMQINVAMRTVDLFPDMVIQMVMIGEESGAIDDMLSKVATIFEQEVDDLVDGLTSLLEPLIMVVLGVLVGGMVVA
MYLPIFKLGDVIG

Nucleotide


Download         Length: 1242 bp        

>NTDB_id=250308 CK911_RS14805 WP_068978945.1 3324275..3325516(+) (pilC) [Aeromonas sp. CU5]
ATGGCTACGCTAGCAAAAAAGAGTAATGCGCCCAAAAAAGTCTTCGCCTTCCGCTGGCACGGGGTCAATCGCAAAGGACA
AAAAGTGTCCGGCGAACTGCAGGCTGACAGCATCAGTACCGTCAAGGCCGAGCTGCGCAAGCAAGGGGTCAACGTCACCA
AGGTCGCCAAAAAATCCCAGGGGCTCTTCTCCAAGGGGGGCGCCAAGATCAAGCCGATGGATATCGCCATCGTGTCACGC
CAGATCACCACCATGCTCTCTGCCGGGGTGCCGCTGGTCCAGAGCCTGCAGATCATCGCCCGCAGCCATGAAAAAGCGGC
AATGCGCGAGCTGATGGGACAAATTGCCTCGGATGTCGAGACTGGCACCCCGATGTCAGAGGCGCTGCGCCGCCATCCCC
GCCACTTTGACGAACTTTATTGCGATCTGGTGGAGGCCGGTGAGCAATCCGGTGCACTGGAGACCATCTACGACCGTATC
GCGACCTACCGGGAAAAATCGGAAGCGCTCAAGTCGAAGATCAAGAAAGCGATGTTTTACCCTGCCATGGTCATGCTGGT
GGCCATTGTGGTGACCTCGATCCTGCTGTTGTTCGTGATCCCCCAGTTCGAAGATATCTTCAAAAGCTTTGGTGCCGAGT
TACCGGCCTTCACCCAGTTTGTCATCGGCATCTCCCGCTTTATGCAGAACTGGTGGTACCTGATTTTTGGCGGTGTGGGG
CTGGGGATCTTCCTCTATGTGCGCGCCTGGCGCGCCTCGCAAACGGTAAAAGACAACACCGACAAGTTTGTACTGACTAT
TCCGGTGGTCGGAATGATCCTGCACAAAGCGGCGATGGCGCGCTTTGCCCGTACCCTGTCGACCACCTTCTCAGCAGGTA
TCCCGCTGGTAGATGCGCTGATTTCGGCAGCTGGCGCCTCCGGCAACTATGTCTATCGTACCGCCGTGCTGGCCATTCGT
AACGAAGTGGTGGCAGGGATGCAGATCAACGTGGCGATGCGCACGGTCGATCTCTTCCCGGACATGGTGATCCAGATGGT
GATGATCGGTGAGGAGTCAGGCGCTATCGATGATATGCTCTCCAAGGTCGCCACCATCTTCGAACAAGAGGTGGATGATC
TGGTCGATGGCCTCACCAGCCTGCTCGAACCCCTGATCATGGTGGTGCTCGGGGTGCTGGTTGGTGGCATGGTGGTCGCC
ATGTACCTGCCCATCTTCAAGCTCGGTGATGTCATTGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(79-201)

Type II secretion system (T2SS), protein F (T2SSF)

(282-404)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A291TLJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.313

95.884

0.54
  pilC Acinetobacter baumannii D1279779

53.75

96.852

0.521
  pilC Legionella pneumophila strain ERS1305867

51.98

97.821

0.508
  pilC Acinetobacter baylyi ADP1

52.645

96.126

0.506
  pilC Vibrio campbellii strain DS40M4

46.377

100

0.465
  pilC Vibrio cholerae strain A1552

47.382

97.094

0.46
  pilG Neisseria meningitidis 44/76-A

39.506

98.063

0.387
  pilG Neisseria gonorrhoeae MS11

39.506

98.063

0.387
  pilC Thermus thermophilus HB27

38

96.852

0.368

Multiple sequence alignment