Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CK911_RS14800 Genome accession   NZ_CP023817
Coordinates   3322328..3324034 (+) Length   568 a.a.
NCBI ID   WP_098970883.1    Uniprot ID   A0A291TL51
Organism   Aeromonas sp. CU5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3317328..3329034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK911_RS14770 (CK911_14770) pdhR 3318469..3319233 (-) 765 WP_042019553.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  CK911_RS14775 (CK911_14775) ampD 3319503..3320087 (-) 585 WP_098970877.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CK911_RS14780 (CK911_14780) - 3320218..3320688 (+) 471 WP_098970879.1 TIGR02281 family clan AA aspartic protease -
  CK911_RS14785 (CK911_14785) nadC 3320692..3321567 (+) 876 WP_098970881.1 carboxylating nicotinate-nucleotide diphosphorylase -
  CK911_RS14795 (CK911_14795) - 3321894..3322328 (+) 435 WP_098972701.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CK911_RS14800 (CK911_14800) pilB 3322328..3324034 (+) 1707 WP_098970883.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  CK911_RS14805 (CK911_14805) pilC 3324275..3325516 (+) 1242 WP_068978945.1 type II secretion system F family protein Machinery gene
  CK911_RS14810 (CK911_14810) pilD 3325620..3326492 (+) 873 WP_098970886.1 A24 family peptidase Machinery gene
  CK911_RS14815 (CK911_14815) coaE 3326496..3327110 (+) 615 WP_098970888.1 dephospho-CoA kinase -
  CK911_RS14820 (CK911_14820) zapD 3327148..3327870 (+) 723 WP_068978942.1 cell division protein ZapD -
  CK911_RS14825 (CK911_14825) yacG 3327883..3328077 (+) 195 WP_042019568.1 DNA gyrase inhibitor YacG -
  CK911_RS14830 (CK911_14830) mutT 3328149..3328553 (-) 405 WP_098970892.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62654.11 Da        Isoelectric Point: 5.4318

>NTDB_id=250307 CK911_RS14800 WP_098970883.1 3322328..3324034(+) (pilB) [Aeromonas sp. CU5]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKHFVTFLIENDILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTIAIGKLLESENDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALAKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSAGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRISGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=250307 CK911_RS14800 WP_098970883.1 3322328..3324034(+) (pilB) [Aeromonas sp. CU5]
ATGACCTCAAGCCCCAATAGCGGCCTGGCGCTAAGCCTGGCCGCCTCTTCACTGATCAGCGAAGAGGACTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAGCACTTTGTCACCTTTCTGATCGAGAACGACATCCTCGACAGCAAGGCGCTGG
CAGATTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGATTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAACTGATTGAGAAACATCACGTACTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
CCCGACCAATGTCTCGGCGTTGGAGGACTTTGGCTTCAGCTTCGGCCTGCATACCGAGGCGCTGCTGGTGGAGGAGAACA
AACTCACCATCGCCATCGGCAAGCTGCTGGAGAGCGAAAACGACGCCCTCGGCATGGAAGAGATCGACGAATCCGAGATC
TCCGAACTCGAAGTATCTGACGAAAACAGCCGGCTCGATGAGAGCGTCAATACCGCCGATGACGACGCCCCCATCGTCAA
GTACATCAACAAAATCCTGATGGATGCCATCAAGCGCGGCGCCTCTGACCTGCACTTCGAGCCCTACGAGTTCAAGTATC
GGGTGCGCTTTCGTATTGATGGCATTCTCCATGAAATCGCCACCCCGCCGGTCAACCTCTCCAATCGCTTCTCCGCCCGA
CTCAAGGTAATGGCGCGGCTCGATATCGCCGAGCGGCGCTTGCCACAAGATGGCCGCATCAAGTTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAACACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGGCTGCTCGACTCCTCAG
CGGCCCGCCTCAATATCGAGCAGCTCGGCTTTGACGACCGGCAGAAGGCGCAATACCTGCGGGCGCTGGCCAAACCGCAG
GGGATGATCCTGGTGACCGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACCAC
AGAGGTGAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAACCAAGTGCAGGTCAACCCCAAGG
CAGGGCTCACCTTCGCCAGCGCCCTGCGCTCCTTCTTACGCCAAGACCCGGACGTGGTGATGGTGGGGGAGATCCGCGAT
CTCGAAACCGCCGAAATCGCCATCAAGGCAGCGCAAACCGGCCACCTGGTGCTCTCGACCCTGCACACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTTAACATCGCCTCCTCAGTGACCCTGATCATGGCCCAGC
GACTGGCTCGCAAACTGTGCGATCACTGCAAAGCGCCAGAAGTTGTACCGGAAGCCGAACTGCTGGAGCTGGGCTTTACC
CCGCAGCAACTGGCAGCTGGTTTGCGGCTGTTCAAGCCGGTTGGCTGCAAAGAGTGCTCGGCGGGCTACAAGGGGCGGGT
CGGTATCTACGAAATCATGCTGATGTCAGAGAACATCGCCAAATTGATCATGCAAGGGGCCAACTCGCTGCAGATCGCCG
CCATTGCCCAGAAAGAGGGGATGCGCACCCTGCGGATCTCCGGCCTGGAGAAGGCCCGGCTCGGCGTCACCAGTCTGGCT
GAAATCAACCGAGTGACCACCAACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A291TL51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.965

100

0.562
  pilB Acinetobacter baumannii D1279779

55.732

99.824

0.556
  pilB Legionella pneumophila strain ERS1305867

56.169

95.599

0.537
  pilB Vibrio cholerae strain A1552

54.578

98.063

0.535
  pilB Vibrio campbellii strain DS40M4

51.767

99.648

0.516
  pilB Vibrio parahaemolyticus RIMD 2210633

51.773

99.296

0.514
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilF Thermus thermophilus HB27

38.879

97.359

0.379
  pilB/pilB1 Synechocystis sp. PCC 6803

38.698

97.359

0.377

Multiple sequence alignment