Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   CPU05_RS05990 Genome accession   NZ_CP023654
Coordinates   1250600..1251025 (-) Length   141 a.a.
NCBI ID   WP_002830426.1    Uniprot ID   -
Organism   Pediococcus acidilactici strain JQII-5     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1213104..1259703 1250600..1251025 within 0


Gene organization within MGE regions


Location: 1213104..1259703
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPU05_RS05775 (CPU05_05795) - 1213104..1213658 (-) 555 WP_087116102.1 hypothetical protein -
  CPU05_RS05780 (CPU05_05800) - 1213686..1214540 (-) 855 WP_087116100.1 hypothetical protein -
  CPU05_RS05785 (CPU05_05805) - 1214886..1216076 (-) 1191 WP_257640360.1 DUF262 domain-containing protein -
  CPU05_RS05790 (CPU05_05810) - 1216219..1216485 (-) 267 WP_141783057.1 hypothetical protein -
  CPU05_RS05795 (CPU05_05815) - 1217053..1218021 (+) 969 WP_260603934.1 site-specific integrase -
  CPU05_RS05800 (CPU05_05820) - 1219323..1219565 (-) 243 WP_024862287.1 hypothetical protein -
  CPU05_RS05805 (CPU05_05825) - 1219683..1220045 (-) 363 WP_002830384.1 phage holin -
  CPU05_RS05810 (CPU05_05830) - 1220058..1220321 (-) 264 WP_002830385.1 hemolysin XhlA family protein -
  CPU05_RS05815 (CPU05_05835) - 1220321..1221484 (-) 1164 WP_002830386.1 GH25 family lysozyme -
  CPU05_RS05820 (CPU05_05840) - 1221496..1221855 (-) 360 WP_002830387.1 hypothetical protein -
  CPU05_RS05825 (CPU05_05845) - 1221870..1222118 (-) 249 WP_002830389.1 hypothetical protein -
  CPU05_RS05830 (CPU05_05850) - 1222160..1222369 (-) 210 WP_002830391.1 hypothetical protein -
  CPU05_RS05835 (CPU05_05855) - 1222362..1222589 (-) 228 WP_002830394.1 hypothetical protein -
  CPU05_RS10375 - 1222582..1225710 (-) 3129 WP_002830395.1 cell envelope integrity protein TolA -
  CPU05_RS05845 (CPU05_05865) - 1225716..1228100 (-) 2385 WP_002830396.1 phage tail spike protein -
  CPU05_RS05850 (CPU05_05870) - 1228165..1229935 (-) 1771 Protein_1133 phage tail domain-containing protein -
  CPU05_RS05855 (CPU05_05875) - 1230012..1234796 (-) 4785 WP_141783058.1 peptidoglycan DD-metalloendopeptidase family protein -
  CPU05_RS05860 (CPU05_05880) - 1234824..1235009 (-) 186 WP_002830399.1 hypothetical protein -
  CPU05_RS05865 (CPU05_05885) - 1235054..1235428 (-) 375 WP_002830400.1 phage tail assembly chaperone -
  CPU05_RS05870 (CPU05_05890) - 1235504..1236193 (-) 690 WP_002830401.1 phage tail protein -
  CPU05_RS05875 (CPU05_05895) - 1236207..1236587 (-) 381 WP_002830403.1 DUF806 family protein -
  CPU05_RS05880 (CPU05_05900) - 1236587..1236994 (-) 408 WP_002830405.1 HK97-gp10 family putative phage morphogenesis protein -
  CPU05_RS05885 (CPU05_05905) - 1236997..1237344 (-) 348 WP_002830406.1 phage head closure protein -
  CPU05_RS05890 (CPU05_05910) - 1237334..1237666 (-) 333 WP_002830407.1 head-tail connector protein -
  CPU05_RS05895 (CPU05_05915) - 1237739..1238971 (-) 1233 WP_141783059.1 phage major capsid protein -
  CPU05_RS05900 (CPU05_05920) - 1238971..1239717 (-) 747 WP_036672383.1 head maturation protease, ClpP-related -
  CPU05_RS05905 (CPU05_05925) - 1239695..1240858 (-) 1164 WP_002830410.1 phage portal protein -
  CPU05_RS05910 (CPU05_05930) - 1240861..1241055 (-) 195 WP_036672386.1 DUF1056 family protein -
  CPU05_RS05915 (CPU05_05935) - 1241045..1242943 (-) 1899 WP_087116079.1 terminase large subunit -
  CPU05_RS05920 (CPU05_05940) - 1242946..1243404 (-) 459 WP_002830412.1 phage terminase small subunit P27 family -
  CPU05_RS05925 (CPU05_05945) - 1243563..1243844 (-) 282 WP_036672389.1 hypothetical protein -
  CPU05_RS05930 (CPU05_05950) - 1243911..1244375 (-) 465 WP_180385071.1 HNH endonuclease -
  CPU05_RS05940 (CPU05_05960) - 1244908..1245369 (-) 462 WP_002830416.1 ArpU family phage packaging/lysis transcriptional regulator -
  CPU05_RS10165 - 1245650..1245808 (-) 159 WP_155105540.1 hypothetical protein -
  CPU05_RS05945 (CPU05_05970) - 1245826..1246011 (-) 186 WP_036672393.1 hypothetical protein -
  CPU05_RS05950 (CPU05_05975) - 1246029..1246301 (-) 273 WP_036672395.1 hypothetical protein -
  CPU05_RS05955 (CPU05_05985) - 1246485..1246856 (-) 372 WP_002830418.1 hypothetical protein -
  CPU05_RS10170 - 1246856..1246993 (-) 138 WP_185833626.1 hypothetical protein -
  CPU05_RS05960 (CPU05_05990) - 1246990..1247400 (-) 411 WP_002830420.1 RusA family crossover junction endodeoxyribonuclease -
  CPU05_RS05965 (CPU05_05995) - 1247381..1248013 (-) 633 WP_036672399.1 DNA-directed RNA polymerase sigma-70 factor -
  CPU05_RS05970 (CPU05_06000) - 1248131..1248946 (-) 816 WP_141783060.1 ATP-binding protein -
  CPU05_RS05975 (CPU05_06005) - 1248927..1249667 (-) 741 WP_002830424.1 conserved phage C-terminal domain-containing protein -
  CPU05_RS05980 (CPU05_06010) - 1249697..1249921 (-) 225 WP_036672402.1 hypothetical protein -
  CPU05_RS05985 (CPU05_06015) - 1249911..1250588 (-) 678 WP_141783061.1 putative HNHc nuclease -
  CPU05_RS05990 (CPU05_06020) ssb 1250600..1251025 (-) 426 WP_002830426.1 single-stranded DNA-binding protein Machinery gene
  CPU05_RS05995 (CPU05_06025) - 1251018..1251224 (-) 207 WP_024863113.1 hypothetical protein -
  CPU05_RS06000 (CPU05_06030) - 1251217..1251942 (-) 726 WP_050755615.1 ERF family protein -
  CPU05_RS06005 (CPU05_06035) - 1251943..1252398 (-) 456 WP_230457018.1 chromosome segregation protein SMC -
  CPU05_RS06010 (CPU05_06040) - 1252624..1252890 (+) 267 WP_036672405.1 hypothetical protein -
  CPU05_RS06015 (CPU05_06045) - 1252847..1253104 (-) 258 WP_036672407.1 hypothetical protein -
  CPU05_RS06020 (CPU05_06050) - 1253204..1253533 (-) 330 WP_002830431.1 DUF771 domain-containing protein -
  CPU05_RS06025 (CPU05_06055) - 1253547..1254269 (-) 723 WP_002830432.1 phage regulatory protein -
  CPU05_RS06030 (CPU05_06060) - 1254283..1254492 (-) 210 WP_065124225.1 helix-turn-helix transcriptional regulator -
  CPU05_RS06035 (CPU05_06065) - 1254748..1255089 (+) 342 WP_065124226.1 helix-turn-helix domain-containing protein -
  CPU05_RS06040 (CPU05_06070) - 1255098..1255505 (+) 408 WP_036672412.1 ImmA/IrrE family metallo-endopeptidase -
  CPU05_RS06045 (CPU05_06075) - 1255568..1256695 (+) 1128 WP_002830434.1 hypothetical protein -
  CPU05_RS06050 (CPU05_06085) - 1256842..1257039 (+) 198 WP_036672414.1 hypothetical protein -
  CPU05_RS06055 (CPU05_06090) - 1257446..1258447 (+) 1002 WP_002830435.1 Abi family protein -
  CPU05_RS06060 (CPU05_06095) - 1258588..1259703 (+) 1116 WP_002830436.1 site-specific integrase -

Sequence


Protein


Download         Length: 141 a.a.        Molecular weight: 15642.35 Da        Isoelectric Point: 6.9816

>NTDB_id=248900 CPU05_RS05990 WP_002830426.1 1250600..1251025(-) (ssb) [Pediococcus acidilactici strain JQII-5]
MINRTILIGRLTKDVELRHTAKGDAVASFTVAVNRQFTNSQGEREADFINCVMWRKAAENFAKYTQKGSLVGIDGRIQTR
SYENQQGQRVYVTEVVADNFSLLDSKPKGNQQNNARQSTTPGDPFANGGQSIDIGDDDLPF

Nucleotide


Download         Length: 426 bp        

>NTDB_id=248900 CPU05_RS05990 WP_002830426.1 1250600..1251025(-) (ssb) [Pediococcus acidilactici strain JQII-5]
ATGATTAATCGAACGATACTAATAGGACGTTTAACTAAAGATGTTGAGCTTCGCCACACAGCTAAAGGCGACGCGGTAGC
TAGTTTTACCGTGGCAGTTAACCGACAGTTTACCAACTCACAGGGTGAACGCGAAGCAGATTTCATCAACTGTGTAATGT
GGCGTAAGGCAGCAGAAAACTTTGCCAAGTACACACAAAAAGGTTCGTTGGTAGGCATTGATGGACGGATCCAAACTCGT
TCCTACGAAAACCAACAAGGGCAGCGAGTTTATGTTACCGAAGTTGTTGCGGATAACTTCTCACTGCTAGATTCGAAACC
AAAAGGCAACCAGCAAAATAACGCACGGCAATCTACAACGCCGGGAGACCCGTTCGCTAATGGCGGGCAGTCAATCGATA
TTGGTGACGACGATTTACCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

60

100

0.723

  ssbA Bacillus subtilis subsp. subtilis str. 168

53.488

100

0.652

  ssbB Bacillus subtilis subsp. subtilis str. 168

62.037

76.596

0.475

  ssbB/cilA Streptococcus mitis NCTC 12261

42.958

100

0.433

  ssbB/cilA Streptococcus pneumoniae TIGR4

42.958

100

0.433

  ssbB/cilA Streptococcus pneumoniae Rx1

42.254

100

0.426

  ssbB/cilA Streptococcus pneumoniae D39

42.254

100

0.426

  ssbB/cilA Streptococcus pneumoniae R6

42.254

100

0.426

  ssbB Streptococcus sobrinus strain NIDR 6715-7

42.254

100

0.426

  ssbB/cilA Streptococcus mitis SK321

42.254

100

0.426

  ssbA Streptococcus mutans UA159

38.732

100

0.39


Multiple sequence alignment