Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CNN82_RS04640 Genome accession   NZ_CP023466
Coordinates   977413..978630 (-) Length   405 a.a.
NCBI ID   WP_096479537.1    Uniprot ID   A0AB33EAA9
Organism   Pseudomonas frederiksbergensis strain KNU-15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 972413..983630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNN82_RS04605 (CNN82_04605) - 973617..973859 (-) 243 WP_028938888.1 DUF2790 domain-containing protein -
  CNN82_RS04615 (CNN82_04615) - 974744..975433 (+) 690 WP_028940362.1 energy-coupling factor ABC transporter permease -
  CNN82_RS04620 (CNN82_04620) - 975497..975712 (+) 216 WP_007938959.1 hypothetical protein -
  CNN82_RS04625 (CNN82_04625) yacG 975722..975922 (-) 201 WP_007938961.1 DNA gyrase inhibitor YacG -
  CNN82_RS04630 (CNN82_04630) coaE 975919..976542 (-) 624 WP_026286732.1 dephospho-CoA kinase -
  CNN82_RS04635 (CNN82_04635) pilD 976539..977411 (-) 873 WP_096479536.1 A24 family peptidase Machinery gene
  CNN82_RS04640 (CNN82_04640) pilC 977413..978630 (-) 1218 WP_096479537.1 type II secretion system F family protein Machinery gene
  CNN82_RS04645 (CNN82_04645) pilB 978633..980333 (-) 1701 WP_019582871.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CNN82_RS04650 (CNN82_04650) - 980560..980979 (+) 420 WP_019582870.1 pilin -
  CNN82_RS04655 (CNN82_04655) - 981303..981659 (+) 357 WP_028940360.1 BON domain-containing protein -
  CNN82_RS04660 (CNN82_04660) - 981833..982129 (-) 297 WP_019582868.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44002.82 Da        Isoelectric Point: 9.9233

>NTDB_id=247261 CNN82_RS04640 WP_096479537.1 977413..978630(-) (pilC) [Pseudomonas frederiksbergensis strain KNU-15]
MAVKAAKISVYAWEGTDRKGAKMTGELTGQNPALIKAQLRKQGINPGKVRKKSASIFSAGKRIKPLDIALFTRQMATMMK
AGVPLLQSFEIIGEGFDNPNMRKLVDEVKQEVAAGNSFAAALRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFQSVFSGFGAELPAFTVMVIGLSEFMQDWWWMILGVLAGGIFGLR
HALKNSPGFRDRTDTWLLKLPLVGALMYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSGIFPTMAIQMTAIGEESGALDDMLDKVASFYEAEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=247261 CNN82_RS04640 WP_096479537.1 977413..978630(-) (pilC) [Pseudomonas frederiksbergensis strain KNU-15]
ATGGCGGTCAAAGCAGCGAAAATCAGCGTGTATGCCTGGGAAGGCACGGATCGCAAAGGTGCGAAAATGACCGGCGAGTT
GACCGGTCAAAATCCCGCGCTGATCAAGGCCCAGTTGCGCAAGCAGGGCATCAACCCGGGCAAGGTGCGGAAAAAGTCCG
CGTCCATTTTCAGTGCCGGTAAGCGCATCAAACCCTTGGACATTGCCCTGTTTACCCGGCAGATGGCGACCATGATGAAA
GCCGGCGTACCGCTCCTGCAATCGTTCGAGATCATCGGCGAAGGCTTCGATAACCCGAACATGCGCAAGCTGGTGGACGA
GGTGAAACAAGAAGTCGCGGCCGGTAACAGCTTCGCTGCGGCCCTGCGCAAAAAGCCCCAGTATTTCGACGAGCTGTACT
GCAACCTGGTGGACGCCGGCGAGCAGGCCGGTGCCCTGGATACCCTGCTGGAACGGGTGGCGACCTACAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGCTGGCCGTGGTGTTTGTCGCAATCATTGTGACCGGCAT
TCTGCTGGTCAAGGTTGTGCCGCAGTTCCAGTCGGTATTCTCGGGGTTCGGCGCCGAACTGCCGGCGTTCACTGTGATGG
TCATCGGCTTGTCGGAGTTCATGCAGGACTGGTGGTGGATGATACTCGGCGTGCTGGCTGGGGGGATCTTCGGCCTGCGC
CATGCCTTGAAGAATTCCCCGGGCTTTCGCGACCGGACGGACACCTGGCTGCTGAAACTGCCATTGGTTGGCGCGCTCAT
GTACAAGTCCGCCGTGGCCCGATACGCCCGCACCCTGTCGACGACGTTTGCCGCCGGCGTGCCACTGGTCGAGGCTCTGG
ATTCAGTCGCAGGCGCGACTGGCAACATCGTGTTCAAGCGAGCGGTGCTACGCATCAAGCAGGACGTTTCCACCGGCATG
CAGCTGAATTTTTCCATGCGTACCTCCGGTATCTTTCCGACCATGGCGATCCAGATGACGGCCATTGGCGAAGAGTCCGG
CGCGCTGGACGACATGCTCGACAAGGTCGCGAGTTTCTACGAGGCCGAGGTGGACAACATGGTCGATAACCTGACCAGCC
TGATGGAACCCTTCATCATGGTGGTGCTGGGGGTTATCGTCGGCGGGCTGGTGGTTGCAATGTACCTGCCCATCTTCCAA
CTCGGCTCAGCGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.815

100

0.748
  pilC Acinetobacter baylyi ADP1

61.029

100

0.615
  pilC Acinetobacter baumannii D1279779

59.314

100

0.598
  pilC Legionella pneumophila strain ERS1305867

53.535

97.778

0.523
  pilG Neisseria gonorrhoeae MS11

42.537

99.259

0.422
  pilC Vibrio cholerae strain A1552

42.929

97.778

0.42
  pilG Neisseria meningitidis 44/76-A

42.04

99.259

0.417
  pilC Vibrio campbellii strain DS40M4

40.876

100

0.415
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment