Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CNN82_RS04645 Genome accession   NZ_CP023466
Coordinates   978633..980333 (-) Length   566 a.a.
NCBI ID   WP_019582871.1    Uniprot ID   A0AB33E5F2
Organism   Pseudomonas frederiksbergensis strain KNU-15     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 973633..985333
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CNN82_RS04615 (CNN82_04615) - 974744..975433 (+) 690 WP_028940362.1 energy-coupling factor ABC transporter permease -
  CNN82_RS04620 (CNN82_04620) - 975497..975712 (+) 216 WP_007938959.1 hypothetical protein -
  CNN82_RS04625 (CNN82_04625) yacG 975722..975922 (-) 201 WP_007938961.1 DNA gyrase inhibitor YacG -
  CNN82_RS04630 (CNN82_04630) coaE 975919..976542 (-) 624 WP_026286732.1 dephospho-CoA kinase -
  CNN82_RS04635 (CNN82_04635) pilD 976539..977411 (-) 873 WP_096479536.1 A24 family peptidase Machinery gene
  CNN82_RS04640 (CNN82_04640) pilC 977413..978630 (-) 1218 WP_096479537.1 type II secretion system F family protein Machinery gene
  CNN82_RS04645 (CNN82_04645) pilB 978633..980333 (-) 1701 WP_019582871.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CNN82_RS04650 (CNN82_04650) - 980560..980979 (+) 420 WP_019582870.1 pilin -
  CNN82_RS04655 (CNN82_04655) - 981303..981659 (+) 357 WP_028940360.1 BON domain-containing protein -
  CNN82_RS04660 (CNN82_04660) - 981833..982129 (-) 297 WP_019582868.1 DUF2845 domain-containing protein -
  CNN82_RS04665 (CNN82_04665) - 982129..985116 (-) 2988 WP_172901167.1 DUF748 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62872.90 Da        Isoelectric Point: 5.5491

>NTDB_id=247262 CNN82_RS04645 WP_019582871.1 978633..980333(-) (pilB) [Pseudomonas frederiksbergensis strain KNU-15]
MNDIALSGLAKQLVLAELITDQSAQQAYQQAQRDRVSLVSYLVQNKLLKSWQVAEVASEHFGMALLDLNCLEKDTQPKGL
VSEKLIRQHHALPLWRRGNKLFVGVSDPSNQQVINDIQFSTGLNTEAILVEEDKLSDAIEKFFDSHATGLEEMADVDLDG
LDIESVDDQKQDAIAGQDTDDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKSYRVRMRTDGILHEVAKPPTQLANRIASRL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVIRILDPSSAQMGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGVNQVNVNPRQGMDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAQTLTRLHNMGIQGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLLKEGFPE
ERIGSFTIYEPVGCNQCNAGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDLQMRKDGFNDLRTSGLLKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=247262 CNN82_RS04645 WP_019582871.1 978633..980333(-) (pilB) [Pseudomonas frederiksbergensis strain KNU-15]
ATGAATGACATTGCCCTTAGCGGTCTGGCCAAGCAATTGGTGCTGGCCGAGCTGATCACTGATCAAAGTGCGCAACAGGC
TTATCAGCAAGCCCAACGCGATCGAGTCTCACTGGTCAGTTATCTGGTGCAAAACAAACTGCTGAAAAGCTGGCAGGTCG
CCGAGGTGGCTTCGGAGCATTTCGGCATGGCCCTGCTGGACCTCAACTGCCTGGAAAAAGACACCCAGCCCAAGGGGCTG
GTCAGCGAAAAACTGATCCGCCAGCACCACGCCCTACCCCTCTGGCGACGCGGCAACAAACTGTTCGTGGGGGTTTCCGA
TCCGAGCAATCAACAGGTCATCAACGACATCCAGTTCAGTACCGGGCTGAACACCGAAGCCATCCTGGTCGAAGAAGACA
AACTCAGCGACGCCATCGAAAAATTCTTCGACAGCCACGCCACCGGCCTGGAAGAGATGGCCGATGTCGACCTCGACGGT
CTGGACATCGAGTCGGTCGACGACCAGAAACAGGACGCCATCGCCGGGCAGGACACCGATGATGCGCCCGTGGTGCGCTT
CGTCCACAAGATGTTGCTCGATGCGATCAAGAGCGGTTCTTCCGACCTGCATTTCGAACCCTACGAGAAATCCTACCGCG
TGCGGATGCGCACCGATGGCATCCTGCATGAAGTCGCCAAGCCACCAACGCAACTGGCCAACCGCATTGCTTCCCGGCTC
AAAGTCATGGCCAGTCTCGACATCTCCGAGCGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCCAAGAGCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATCCTCGATCCGTCCAGTG
CGCAAATGGGCATCGATGCCCTCGGCTACGAGCCCGAGCAGAAAGACCTGTACATGGCCGCCCTCAAGCAACCACAGGGG
ATGATCCTGGTGACCGGGCCCACCGGTTCAGGCAAGACCGTGTCGCTCTATACCGGGCTGAACATCCTCAACACCGTCGA
CATCAACATCTCCACTGCCGAAGACCCGGTGGAAATCAACATGGAAGGCGTCAACCAGGTCAACGTCAATCCCAGGCAAG
GAATGGATTTCGCCCAGGCGTTGCGCTCGTTTCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAAATCCGCGACCTC
GAAACTGCCGAAATTGCCATCAAAGCCGCACAGACCGGGCACATGGTGCTTTCAACCCTGCACACCAACAGCGCCGCGCA
AACCCTGACCCGCCTGCACAACATGGGGATTCAGGGCTTCAACATTGCGACCTCGGTCAGCCTGATCATTGCCCAGCGCC
TGGCGCGCAAGTTGTGCAGCCATTGCAAGAAACCCATCGAGATTCCCCGCGAGACGCTGCTCAAGGAAGGCTTCCCCGAG
GAACGCATCGGCTCGTTCACGATCTATGAACCGGTCGGCTGTAATCAATGCAACGCCGGCTACAAAGGGCGAGTGGGGAT
TTATGAAGTGGTAAAGAACACCCCTGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCACTGGAGATCGACCTGCAGA
TGCGCAAGGACGGCTTCAACGACCTGCGCACGTCGGGCTTACTCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AATCGGGTCACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.025

100

0.581
  pilB Acinetobacter baumannii D1279779

57.979

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

56.36

100

0.564
  pilB Vibrio cholerae strain A1552

50.265

99.823

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50

98.233

0.491
  pilB Vibrio campbellii strain DS40M4

48.421

100

0.488
  pilF Neisseria gonorrhoeae MS11

48.49

99.47

0.482
  pilF Thermus thermophilus HB27

36.219

100

0.362

Multiple sequence alignment