Detailed information NTG0247242

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: CNO08_RS19030
• Genome accession: NZ_CP023465
• Coordinates: 4760659..4761072 (+)
• Length: 137 a.a.
• NCBI ID: WP_036104562.1
• Uniprot ID: A0A108UD59
• Organism: Lysobacter capsici strain KNU-14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	4761267..4771379	4760659..4761072	flank	195

Gene organization within MGE regions

Location: 4760659..4771379

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CNO08_RS19030 (CNO08_19030)	pilA2	4760659..4761072 (+)	414	WP_036104562.1	pilin	Machinery gene
CNO08_RS19035 (CNO08_19035)	pilA2	4761267..4761677 (+)	411	WP_096415524.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
CNO08_RS19040 (CNO08_19040)	-	4761806..4763698 (+)	1893	WP_157754139.1	hypothetical protein	-
CNO08_RS19045 (CNO08_19045)	-	4763695..4765707 (+)	2013	WP_157754140.1	hypothetical protein	-
CNO08_RS19050 (CNO08_19050)	-	4765926..4766900 (+)	975	WP_157754141.1	hypothetical protein	-
CNO08_RS19055 (CNO08_19055)	-	4766887..4767636 (+)	750	WP_096415536.1	class I SAM-dependent methyltransferase	-
CNO08_RS19060 (CNO08_19060)	-	4767633..4768694 (+)	1062	WP_096415539.1	NAD(P)-binding domain-containing protein	-
CNO08_RS19065 (CNO08_19065)	-	4768674..4769528 (-)	855	WP_157754142.1	GNAT family N-acetyltransferase	-
CNO08_RS19070 (CNO08_19070)	-	4769521..4770678 (-)	1158	WP_096415545.1	glycosyltransferase	-
CNO08_RS19075 (CNO08_19075)	-	4770675..4771379 (-)	705	WP_051547075.1	class I SAM-dependent methyltransferase	-

Sequence

Protein

• Length: 137 a.a.
• Molecular weight: 14146.26 Da
• Isoelectric Point: 7.5886

>NTDB_id=247242 CNO08_RS19030 WP_036104562.1 4760659..4761072(+) (pilA2) [Lysobacter capsici strain KNU-14]
MKKQQGFTLIELMIVIAILGILIAIALPAYQDYTIRTKNAECLNIAAAAKLAVAETAQDRGTLAGVTEALTGYQFAASSY
CASITIANGGAITATTQATGATPPAVLTLRPTGGNGRLEWACSGTGRDSQLPAECRN

Nucleotide

• Length: 414 bp

>NTDB_id=247242 CNO08_RS19030 WP_036104562.1 4760659..4761072(+) (pilA2) [Lysobacter capsici strain KNU-14]
ATGAAGAAGCAGCAAGGCTTTACCCTCATCGAACTGATGATCGTCATCGCGATCCTCGGCATCCTGATCGCCATCGCTCT
GCCGGCGTACCAGGACTACACGATCCGTACCAAGAACGCTGAGTGCCTGAACATCGCCGCCGCCGCCAAGCTGGCCGTCG
CCGAAACCGCTCAGGATCGCGGCACCCTGGCGGGCGTCACCGAAGCTCTGACCGGCTACCAGTTCGCCGCCAGCAGCTAC
TGCGCGTCGATCACCATCGCCAACGGCGGCGCCATCACCGCGACCACGCAGGCCACCGGCGCCACCCCGCCGGCCGTGCT
GACCCTGCGTCCGACCGGCGGCAACGGCCGTCTGGAATGGGCTTGCAGCGGCACCGGCCGTGACTCGCAGCTGCCGGCCG
AATGCCGCAACTGA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (36-136)

• Prokaryotic N-terminal methylation motif (N_methyl) (1-27)

3D structure

• AlphaFold DB: A0A108UD59

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	51.095	100	0.511
pilA2	Legionella pneumophila strain ERS1305867	49.635	100	0.496
pilA	Ralstonia pseudosolanacearum GMI1000	39.264	100	0.467
comP	Acinetobacter baylyi ADP1	40.667	100	0.445
pilE	Neisseria gonorrhoeae strain FA1090	36.875	100	0.431
pilE	Neisseria gonorrhoeae MS11	36.25	100	0.423
pilA	Pseudomonas aeruginosa PAK	37.748	100	0.416
pilA/pilAI	Pseudomonas stutzeri DSM 10701	39.416	100	0.394
pilA	Haemophilus influenzae 86-028NP	36.691	100	0.372